Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 87 for “"Haemolysis"”.
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A theoretical approach to the prediction of haemolysis in centrifugal blood pumps
… damage prediction theory was demonstrated for haemolysis prediction in a commercial centrifugal blood pump. Particle loading-time functions were determined with a 3-dimensional numerical flow analysis of the entire pump domain by means of assigning scalar stress values to particle streaklines. …
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Diamphotoxin : the arrow poison of the !Kung Bushmen
… contained a 3,7 S cationic protein which caused haemolysis and, after intramuscular injection, local paralysis followed by death. By intravenous lethality assay, one 200 mg pupa contained 20 000 mouse lethal doses. Assays for the toxin were developed based upon haemolysis in vitro and lethality …
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Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles
… GNPs were haemocompatible as they induced 0.92 % haemolysis at the highest concentration (500 μL) in haemolysis assay compared to 99 % haemolysis by positive control. In conclusion, the results of this study show that CAP-GNPs possess good antioxidant and anticholinesterase activities, in addition …
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Evaluation of bioactivity of QUB1977 from the skin secretion of the frog, Phyllomedusa camba
… of 4, 32 and 4 µM, respectively. The haemolysis of this peptide was not significant at low concentrations but had a considerable increase at high concentrations. The concentration of the test peptide which induced 50% haemolysis (HC50) was 23 μM. The results indicated that the …
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Discovery, structural characterisation and targeted engineering of bioactive peptides from amphibian skin secretion
… however, it induced significant cytotoxicity and haemolysis. To eliminate these side effects and reveal the function of the ‘Rana Box’ domain of QUB-3307, two truncated analogues, QUB-2531 and QUB-1376, were designed. QUB-2531 produced a low degree of cytotoxicity and haemolysis without decreasing …
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Welfare issues in Italian heavy pig production
… were found to have a variable degree of spurious haemolysis. The release of free haemoglobin can bias the quantification of several analytes. Therefore, a further study evaluated the effect of physical haemolysis in 3 aliquots (on a scale of 1+ to 3+) of 30 non-haemolytic sera, in order to assess …
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Discovery and bioactivity assessment of a novel peptide from the skin secretion of the Senegal running frog Kassina senegalens
… this peptide demonstrated less than 10% haemolysis at a concentration of 256µM. These results suggest that this novel peptide is worthy of attention as a new antibacterial lead due to its potent inhibitory and bactericidal effects against Gram-ve bacteria, along with a low level of …
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Identification and functional study of a bioactive peptide QUB-2413 from the skin secretion of the Red-Eyed Tree Frog, Agalychnis callidryas
… from 10 -6 M to 10 -4 M. With regard to haemolysis assay, QUB-2413 had no more than 10% haemolysis activity at 512 µM. Although the biological activities of QUB-2413 are not ideal, it is still expected to become a safe anti-positive bacteria drugs in the future after its antimicrobial …
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Assessing bioactivity of a peptide from the skin secretion of the central American red-eyed leaf Frog, Agalychnis callidryas
… with an IC50 value of 8.3 μM. With regard to the haemolysis assay, QUB-2428 had no more than 5% haemolysis activity at a 512 μM concentration. With further modifications and experiments, QUB-2428 may be found to have better antimicrobial and anticancer functions in future …
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An antimicrobial peptide QUB-1925 from scorpion venom of Androctonus bicolor: molecular cloning of biosynthetic precursor-encoding cDNA and bioactivity study
… was used to perform antimicrobial assays, haemolysis assay, and anti-tumour cell assays. <br/><br/>QUB-1925 presented inhibition effect on gram-positive bacteria, S. aureus, and gram-negative bacterium, E. coli, but little effect against the fungi, C. albicans. More importantly, QUB-1925 …
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Bioactivity assessment of peptide QUB1471 from the skin secretion of the Chinese torrent frog (Amolops wuyiensis)
… was identified. Trypsin inhibitory assay, haemolysis assay and antimicrobial assays were performed to evaluate the biological activities of QUB1471. Based on the results, QUB1471 was an effective trypsin inhibitor and its Ki value was 0.8657 µM. However, QUB1471 did not inhibit the growth …
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The discovery and functional study of an antimicrobial peptide QUB-1955 from the skin secretion of the northern orange-legged leaf frog, phyllomedusa hypochondrialis
… assays, anti-cancer cell assays and haemolysis assay). <br/><br/>In the order of potency, QUB-1955 had relatively potent antibacterial activity against Gram- positive bacteria (S. aureus), while it was weak against Gram-negative bacteria (E. coli), and had no activity against yeast …
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Molecular cloning and bioactivity assessment of a bioactive peptide QUB-1509 from the skin secretion of the Asian frog Hylarana guentheri
… and U251MG human cancer cell lines. In the haemolysis assay, QUB-1509 showed low cytotoxicity towards horse red blood cells with no more than 15% haemolysis at 512 μM. These results have laid the foundation for the development of QUB-1509 as a potential antibacterial therapeutic.
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Identification, isolation and bioactivity assessment of a novel peptide from the skin secretion of the Tiger-striped Leaf Frog (Phyllomedusa tomopterna)
… 1.33 %, and 3.33 %, respectively. The outcome of haemolysis assays showed a moderate 10.65 % haemolysis at a concentration of 128 μM. <br/><br/>According to previous experiments, the novel peptide QUB-2982 from the dermaseptin family displayed potent bacteriostatic and bactericidal activities …
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Identification and functional study of the bioactive peptide QUB-2830 from the skin secretion of the blue-side leaf frog, Agalychnis annae
… cancer cells at a concentration of 10-4 M. In a haemolysis assay, QUB-2830 showed haemolytic activity at concentrations between 16 ~ 128 μM. In summary, QUB-2830 exhibited a remarkable antimicrobial ability and may be expected to become a safe antimicrobial drug in the future after its haemolysis …
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Bioactivity assessment of a bioactive peptide, QUB-2022, isolated from the skin secretion of the tiger-striped leaf frog, (Phyllomedusa tomopterna)
… peptide was found to possess a high degree of haemolysis (63%) while showing activity against E.coli, whereas negligible haemolytic effects toward normal cells of this peptide were observed at the MIC values for S. aureus and C. albicans. However, the half maximal haemolysis concentration …
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QUB-3170: a novel peptide with antimicrobial activities from the skin secretion of Phyllomedusa tarsius
… tested for their anticancer, antimicrobial, <br/>haemolysis activity. <br/>The results of the research showed that it was an activity to Gram-positive，Gram-negative bacteria and fungi. The MIC of QUB-3170 and QUB-2987 to S. aureus, E. Coli and C. albicans was at 8, 4, 32 μM, 8, 8, 32 μM, the MBC …
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The discovery of the novel peptide QUB-2049 from the skin secretion of Odorrana versabilis
… ATCC CRM 10231) (MIC = 128 μM). In the haemolysis assay peptide QUB-2049 showed an obvious haemolytic activity (% haemolysis >10) at concentrations ≥16 μM. The result of the antiproliferative assay showed that QUB-2049 had inhibitory activity against ATCC H838 cells at a concentration of …
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Assessment of antimicrobial and antiproliferative activities of a bioactive peptide from the skin secretion of white-lined leaf frog
… subject to antimicrobial, antiproliferative and haemolysis tests to ascertain its bioactivities. This peptide was found to exhibit antimicrobial activity against S. aureus, E. coli and C. albicans with MIC values of 2 µM, 2 µM and 64 µM, respectively. Also, this peptide possessed an …
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Identification and Molecular Cloning of A Bioactive Peptide from the Defensive Skin Secretion of the Purple-Sided Leaf Frog, Phyllomedusa baltea
… Antimicrobial assays, anticancer assays and a haemolysis assay, were performed to evaluate the biological activities of QUB2492. It demonstrated that QUB2492 did not inhibit the growth of the three tested microorganisms and was not capable of inducing haemolysis, at a concentration up to 512 …
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