Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Haematopoietic cells"”.
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Mechanisms for Targeting of Proteins to Secretory Lysosomes of Haematopoietic Cells
The cells of the innate immune system are essential for the host's first line defence against infection. Among these cells the neutrophil granulocyte has a central role by the production of antibiotic proteins and peptides. These are targeted for storage in secretory lysosomes and other neutrophil …
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The role of Nbeal2 in the homeostasis and retention of granules in haematopoietic cells
… role in essential granule-related processes in haematopoietic cells. Through the NBR-RD GPS study, detailed clinical phenotypes were compared and patient blood samples were examined by Sysmex, RNA-Seq, and mass spectrometry analysis. The study revealed novel clinical phenotypes, including an …
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The Role of Gata3 in Blood Stem Cell Emergence
The first definitive haematopoietic stem cells (HSCs) produced during embryonic development are generated from a specialised subset of endothelial cells known as haemogenic endothelium. Recently, it was reported that Gata3 plays a dual role in the development of sympathetic nervous system and …
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Stimulation of donor t-cells with leukemic blast-loaded dendritic cells for the generation of leukemia-specific immunity in the context of allogeneic stem cell transplantation
… by specific anti-leukemia donor lymphocytes cells, known as graft versus leukemia effect (GVL). This effect can be induced by the infusion of donor lymphocytes (DLI). Adoptive immunotherapy involving DLI, the current approach in relapsed allogeneic transplant patients, leads in this situation …
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Investigating a co-operative link between TGF-β and Lyn kinase in Chronic Myeloid Leukaemia
… characterised by uncontrolled proliferation of haematopoietic cells driven by the fusion gene Bcr-Abl. The advent of a mechanism specific kinase inhibitor, Imatinib, targeting the constitutively active Bcr-Abl kinase has paved the way for new treatment strategies. However, resistance emerges, …
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Investigations into the Biological Roles of the E3 ligase Ariadne 2/TRIAD1
… cellularity and increased apoptosis, however haematopoietic cells are capable of differentiating into myeloid and granulocytic progenitors and can fully reconstitute lethally irradiated Rag1-/- recipient mice. These Rag1-/-Arih2-/- chimeras recapitulate the lymphoproliferative disorder …
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A Non-Canonical Function for NLRP3 and AIM2 in Kidney Diseases
… that NLRP3 or AIM2 is expressed in non-haematopoietic cells such as tubular epithelial cells (TEC) in the kidney. The central hypothesis of this thesis is that NLRP3 and AIM2 regulate host response to renal injury in the kidney. Primary mouse TEC lacking Nlrp3 displayed reduced caspase-8 …
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Novel roles of KAT2A chromatin complexes ATAC and SAGA in normal and malignant haematopoiesis
… of blood cell types originates from multipotent haematopoietic stem cells (HSCs). The blood system has long been an attractive model to explore how cell fate decisions are conducted at the molecular level, to generate the vast diversity of blood cell types. Blood cell fate choice is for the most …
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Characterisation of protease activated receptors
… of hPAR-2 on human umbilical vein endothelial cells, keratinocytes and granulocytes. The expression of both PAR-2 and thrombin receptor were analysed using Northern analysis in a wide variety of murine haematopoietic cell lines. This analysis revealed the widespread distribution of the thrombin …
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Molecular Characterisation and Therapeutic Targeting of NPM1-mutant Acute Myeloid Leukaemia
… describes my work to characterise NPM1-mutant cells at the molecular level using different omics approaches with the key goal of identifying those NPM1c-driven changes that can be exploited for translational applications. We first examined the effects of isolated NPM1c mutations on the proteome …
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Effect of pomegranate extracts on apoptosis and cell cycle in haematological malignancies.
… by the uncontrolled proliferation of haematopoietic cells and progressive accumulation of these cells within the bone marrow (BM) and secondary lymphoid tissues. The exact cause of leukaemia remains unknown. Leukaemia is a major problem worldwide affecting many people each year. …
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COP9 signalosome subunit knockdown in K562 provides novel insight into the function and potential regulation of the CSN
… COP9 signalosome (CSN) has been studied in human cells, little is known of its role in haematopoietic cells or of any potential contribution to leukaemogenesis. In this study the deneddylase catalytic subunit CSN5 and the non-catalytic subunit CSN2 were knocked down in the human haematopoietic …
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Investigating the anti-leukaemic effect of tamoxifen in the myeloproliferative neoplasms
… effective on eradiating JAK2<sup>V617F+</sup> haematopoietic stem and progenitor cells (HSPCs) in preclinical mouse MPN models, which prompted a Phase-II clinical study (Effects of TAMoxifen on the Mutant Allele Burden and Disease Course in Patients with MyeloprolifeRatIve Neoplasms, TAMARIN). …
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Genetic programming of human iPSC-derived macrophages provides a tool to study the Erythroblastic Island niche in vitro
… techniques, such as induced pluripotent stem cells (iPSCs), represents a valuable tool in the macrophage field. As a proof of principle, we used a human iPSC-line that was targeted in the safe harbour AAVS1 locus to express the fluorescent protein ZsGreen constitutively (SFCi55-ZsG). We …
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The role of GATA-1 isoforms in haematopoiesis
GATA-1 is a key haematopoietic transcription factor which plays a pivotal role in differentiation of the erythroid, megakaryocytic, eosinophilic, mast cell and dendritic cell lineages. Since its initial cloning and characterisation in 1989 a huge amount of information has been gathered on the …
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Role of PABP4 in intrauterine growth restriction
… PABP4 was expressed in decidualised stromal cells and in invading trophoblasts (conceptus-derived). It was also expressed within the vast majority of trophoblasts in the junctional zone. However, qualitative analysis with markers for the major cell types within the placenta, ruled out the …
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The interplay of global chromosomal organisation, promoter-enhancer interactions and transcription
All somatic cells within an organism contain the same genetic material, yet they display pronounced differences in function and morphology. Precise control of gene expression is of fundamental importance to allow cells to properly develop, maintain homeostasis, and respond to external stimuli. The …