Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"HaCaT cells"”.
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RegulationofGap Junction Protein,Connexins in Immortalized Human Keratinocytes Cells(HaCaTcells)
Intercellular communication for function of cells and tissues is very important as the intracellular communication system. Gap junctions are ordered arrays of intercellular plasma membrane channels that directly link the cytosols of two adjoining cells. Gap junctional intercelluar communication …
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A Role for the HSV Tegument Protein pUL16 in Modulating Cellular Energy Metabolism during HSV-2 Infection
… null mutant (Δ16), infected human keratinocytes (HaCaT). Mitochondria in WT infected cells exhibited elongated morphology contributing to a more tubular mitochondrial network associated with promotion of mitochondrial function, while mitochondria in Δ16 infected cells exhibited punctate and …
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Repression of Protein Kinase C Delta in Human Squamous Cell Carcinomas by Ras, Fyn and NF-Kappa B Signaling
… we used Laser Capture Microdissection to isolate cells for RNA and DNA analysis from 3 normal epidermises and 14 human SCCs with low PKC-delta protein. Using this more selective approach, we found the tumor suppressor PKC-delta is lost at the mRNA level in human SCCs, and that the PKC-delta gene …
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Screening of radioprotective agents using zebrafish and Therapeutic effects and protective mechanism of radioprotective candidates on radiation-induced oral mucositis
… damage in the human keratinocyte cell line HaCaT and in an in vivo zebrafish model. Radiation inhibited HaCaT cell proliferation and migration in a cell viability assay and wound healing assay, respectively. KRG protected against these effects. KRG attenuated the radiation-induced …
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Development of a novel cell traction force transducer based on cholesteryl ester liquid crystals. Characterisation, quantification and evaluation of a cholesteryl ester liquid crystal based single cell force transducer system.
… forces of human keratinocyte cell lines (HaCaTs), in which the length of the deformation line induced represents the intensity of the CTF exerted. The physical properties of the cholesteryl ester based lyotropic liquid crystals (LLC) were characterised by using polarising microscopy, …
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An examination of the role of IFI16 in detecting viral DNA in human immortalised keratinocytes
… infection in human immortalised keratinocytes (HaCaT). The cGAS-STING pathway was examined in IFI16(-/-) HaCaT cell lines to conclusively determine the contribution of IFI16 to DNA sensing. IFI16(-/-) HaCaT cell lines produced drastically reduced levels of interferon-b, interferon-stimulated …
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Functional study of Gap Junctional Intercellular Communication in Human Keratinocyte Cell Line
… and a highly diffenrentiating keratinocyte(HaCaT) cell line was used to find substances that can change the GJIC function via fuctional study using scrape-loading dye transfer(SLDT) method. Toxic drug level of acetic acid , H₂O₂, dexamethasone, Epicatechin epigallocatechin-3-gallate, …
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Exploiting the potential of foam nests produced by Engystomops pustulosus
… density, resistant to shear and is non-toxic to HaCaT cells. The foams drug release capabilities were tested by loading the foam with model hydrophilic and hydrophobic dyes and measuring their release profiles. The foam delivery potential was then tested with an antibiotic, rifampicin, …
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PIKK-dependent activation of the DNA damage response by long wavelength ultraviolet radiation
… the persistence of UVinduced DNA damage in skin cells. This project used HaCaT cells to investigate UVA and its ability to generate double strand breaks, and how the DDR is activated as a result. Cells exposed to 100 KJ m-2 UVA displayed significant amounts of DSBs, which were largely repaired …
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Migration Stimulating Factor, the search for inhibitors.
… immortalised human keratinocyte cell line (HaCaT) and endothelial cells (ENDO 742) specifically when exhibiting a cobblestone phenotype.<br/>The CM from the HaCaT keratinocyte line was fractionated by both molecular weight and ionic charge, followed by sequence analysis which identified the …
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Regulation and Targeting of Fyn in Human Cutaneous Squamous Cell Carcinoma
… Introduction of active H-Ras(G12V) into the HaCaT human keratinocyte cell line resulted in upregulation of FYN mRNA (200-fold) and protein, but did not increase the expression of other SFKs. We identified two nucleotides 74 bases upstream of the human FYN gene transcription start site as …
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Preparation of polysaccharide-based nanoemulsions to reduce melanoma drug resistance and inflammation markers
… effect on melanoma (A375) and keratinocyte (HaCaT) cells was conducted. The “pullulan-chitosan” formulation was taken forward with folate-modification and used to target A375 cells. Subsequently, the melanocortin type 1 and 3 receptor agonists BMS-470539-dihydrochloride and [DTrp8]-γ-MSH were …
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The effect of environmental stress and selective glucocorticoid receptor modulators on chicken and human leukaemia cells
… that the desired apoptotic effect on cancer cells and anti-inflammatory properties of GCs are due to the GR’s mediated trans-repression function, and that genes positively regulated by GR may mediate unwanted GCs effects. Thus, this study aimed to investigate compounds that would potentially …
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Molecular and microscopical analysis of pathogenic streptococci - studies on surface proteins interacting with human cells and extracellular matrix
… to and internalize into human keratinocytes (HaCaT) cells in a time dependent manner and we were able to identify non-muscle myosin IIA as a potential cellular ligand for Slr. We could further confirm the presence of non-muscle myosin IIA in HaCat cell lysate and that Slr binds to non-muscle …
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NANOFORMULATION OF BUDDLEJA DAVIDII EXTRACT: DEVELOPMENT AND CHARACTERIZATION OF LIPOSOMES AND NANOFIBERS FOR WOUND HEALING APPLICATION
… effects were investigated in human keratinocyte (HaCaT) cells. The liposomal formulations exhibited suitable physicochemical properties, high encapsulation efficiency, and good colloidal stability over time. Encapsulation preserved the antioxidant activity of the extract and ensured excellent …
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Characterisation of surfactant protein a as a novel prophylactic means against oncogenic HPV infections
… infection of the human keratinocyte cell line, HaCaT. Additionally, we aimed to study SP-A's ability to agglutinate HPV-PsVs and to assess potential consequences of this SP-A coating on immune cell recognition and elicited immune responses in human-derived immune cells. Our study demonstrated …
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Novel Polyhydroxyalkanoate blends: their characterisation and possible applications
… the attachment, growth and proliferation of the HaCaT cells. However, biocompatibility was better for the P(3HO)/P(3HB) 5:1 film and the topological features of this film led to improved cell attachment and proliferation. SEM analysis confirmed that the HaCaT cells had been able to grow and …
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Biosynthesis of polyhydroxyalkanoates and its medical applications
… the attachment, growth and proliferation of the HaCaT cells. However, biocompatibility was improved for the P(3HO)/n-BG composites. In vitro degradation studies revealed the films both neat and composite underwent hydrolytic degradation which started at the surface and that aged with time. …
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Antibacterial activity and mode of action of the antimicrobial peptide CPF-MW1 against Escherichia coli ATCC 700928
… against both human erythrocytes (10 ± 4%) and HaCaT cells (25 ± 5%) at 88.9 μM, a concentration 16-fold higher than the MBC. Mode of action studies revealed that CPF-MW1 effectively eradicates planktonic E.coli within 90 minutes, mediated by cytoplasmic membrane permeabilisation. Scanning …
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