Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 60 for “"HaCaT"”.
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A poli-ADP-riboziláció szerepe a HaCaT sejtek UVB sugárzás okozta sejthalálban
A poli-ADP-riboziláció szerepének vizsgálata HaCaT sejtvonalon UVB sugárzás hatására bekövetkező sejthalál során. Különböző PARP gátlószerek hatásának vizsgálata.
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Regulation and Targeting of Fyn in Human Cutaneous Squamous Cell Carcinoma
… Introduction of active H-Ras(G12V) into the HaCaT human keratinocyte cell line resulted in upregulation of FYN mRNA (200-fold) and protein, but did not increase the expression of other SFKs. We identified two nucleotides 74 bases upstream of the human FYN gene transcription start site as …
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RegulationofGap Junction Protein,Connexins in Immortalized Human Keratinocytes Cells(HaCaTcells)
… spontaneously immortalized epidermal cell line (HaCaT) and cultured cholesteatoma cells. If it is possible to control the intercellular communication system of cholesteatoma, in other words, if we inhibit the Cx expression of cholesteatoma, it will be able to inhibit the advancement of …
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An examination of the role of IFI16 in detecting viral DNA in human immortalised keratinocytes
… infection in human immortalised keratinocytes (HaCaT). The cGAS-STING pathway was examined in IFI16(-/-) HaCaT cell lines to conclusively determine the contribution of IFI16 to DNA sensing. IFI16(-/-) HaCaT cell lines produced drastically reduced levels of interferon-b, interferon-stimulated …
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The Studies for the Preparation of Platelet Lysate (PL) and the Effects of Platelet Lysate (PL) on the Proliferations for Microbial and the Human derived cells
… performing cell culture to produce PL by using HaCaT cell lines which are human derived cells. To observe the antibacterial effect, PRP and platelet poor plasma (PPP) from 20 volunteers were separated from the blood treated with CPDA-1 anti-coagulant. The PRP was concentrated to 8 times of the …
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Migration Stimulating Factor, the search for inhibitors.
… immortalised human keratinocyte cell line (HaCaT) and endothelial cells (ENDO 742) specifically when exhibiting a cobblestone phenotype.<br/>The CM from the HaCaT keratinocyte line was fractionated by both molecular weight and ionic charge, followed by sequence analysis which identified the …
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An examination of the bioactive lipids involved in skin cell inflammation and in response to ultraviolet radiation. Effect of n-3 polyunsaturated fatty acid supplementation on red blood cell and human dermal fatty acid and production of eicosanoids by HaCaT keratinocytes and 46BR.1N fibroblasts following exposure to UVR.
… for fatty acids by gas chromatography (GC). HaCaT keratinocytes and 46BR.1N fibroblasts were cultured and treated with 10 and 50μM of either EPA, or DHA or oleic acid (OA) for 72h and exposed to 15 and 50 mJ/cm2. Cell viability was measured by the MTT assay and cell apoptosis by a …
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Repression of Protein Kinase C Delta in Human Squamous Cell Carcinomas by Ras, Fyn and NF-Kappa B Signaling
… immortalized human keratinocytes (HaCaT-Ras), which have selective down-regulation of the PKC-delta isoform at both protein and mRNA levels. Ras significantly repressed human PKC-delta promoter activity in HaCaT cells (85% reduction, p<0.05). Mutagenesis and ChIP studies of the …
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Functional study of Gap Junctional Intercellular Communication in Human Keratinocyte Cell Line
… and a highly diffenrentiating keratinocyte(HaCaT) cell line was used to find substances that can change the GJIC function via fuctional study using scrape-loading dye transfer(SLDT) method. Toxic drug level of acetic acid , H₂O₂, dexamethasone, Epicatechin epigallocatechin-3-gallate, …
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Fructose 1,6-diphosphate ameliorates UV-induced skin inflammation
… 알려져 있다. 피부세포에서는 최근 human keratinocyte cell line (HaCaT)에서 FDP가 UVB 유도 세포내 H2O2 및 prostaglandin 생성을 유의적으로 억제한다고 보고된 바 있다. 본 연구에서는 FDP가 자외선에 의해 유도되는 염증반응 및 산화적 스트레스를 억제할 수 있는지를 피부세포배양 모델과 in vivo 동물시험을 통해 검토하고자 하였다. FDP는 HaCaT keratinocyte에서 UVB에 의해 유도되는 세포사를 억제하였으며, COX-2의 발현 및 MMP-2,9의 활성을 억제하였다. …
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Characterisation of surfactant protein a as a novel prophylactic means against oncogenic HPV infections
… infection of the human keratinocyte cell line, HaCaT. Additionally, we aimed to study SP-A's ability to agglutinate HPV-PsVs and to assess potential consequences of this SP-A coating on immune cell recognition and elicited immune responses in human-derived immune cells. Our study demonstrated …
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Screening of radioprotective agents using zebrafish and Therapeutic effects and protective mechanism of radioprotective candidates on radiation-induced oral mucositis
… damage in the human keratinocyte cell line HaCaT and in an in vivo zebrafish model. Radiation inhibited HaCaT cell proliferation and migration in a cell viability assay and wound healing assay, respectively. KRG protected against these effects. KRG attenuated the radiation-induced …
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A Role for the HSV Tegument Protein pUL16 in Modulating Cellular Energy Metabolism during HSV-2 Infection
… null mutant (Δ16), infected human keratinocytes (HaCaT). Mitochondria in WT infected cells exhibited elongated morphology contributing to a more tubular mitochondrial network associated with promotion of mitochondrial function, while mitochondria in Δ16 infected cells exhibited punctate and …
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Untersuchungen zur Wirkung von 2,2´-Dichlordiethylsulfid (Schwefellost) in primären und immortalisierten epithelialen Zelllinien
… epitheliale Zelllinien (A 549, SCL II, HaCaT und NHEK) wurden eingesetzt, um den Effekt von Schwefellost auf Proliferationsverhalten, Vitalität und Zytokin-Sekretion näher zu untersuchen. In den Versuchen konnte gezeigt werden, dass eine Schwefellost-Exposition bei allen vier …
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Development and Characterisation of an Immunocompetent Three-Dimensional Oral Mucosal Infection Model
… were formed using primary (POK) or immortalized (HaCaT) keratinocytes seeded upon a human gingival fibroblast (HGF) embedded type-1 collagen matrix. Later, HaCaT based models (3DOMMs) were further developed to incorporate THP-1 pro-monocyte cells into the model matrix producing a novel …
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BRAZILIAN PROPOLIS: A MULTIFACETED NATURAL PRODUCT WITH HEALING PROPERTIES - A COMPREHENSIVE STUDY ON THE ABILITY TO IMPROVE CUTANEOUS WOUND HEALING THROUGH HIF-1 MODULATION
… brasiliane verde e rossa in cheratinociti (HaCaT) e fibroblasti dermici (HDF) umani, esplorando la capacità di modulare la via di segnalazione di HIF-1 per corroborarne l’applicazione come innovativi agenti di guarigione. La preliminare caratterizzazione chimica condotta mediante HPLC-UV, …
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Development of an electronic aerosol atomisation system for generating three-dimensional (3D) cells in microencapsulations and microtissues characterisation
… microscopy (FE-SEM) imaging. Keratinocytes (HaCaT) and Oral Squamous Cell Carcinoma (ORL-48) cells at cell densities of 3 × 107 and 9 × 107 cells/ml, respectively were applied for encapsulation and successfully grew into microtissues after 16 days of culture. The fourier transform infrared …
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Stress and Growth Related Keratinocyte Pathways
… in the non-tumorigenic keratinocyte cell line HaCaT. Regulation of IEX-1 gene expression by the EGF-Receptor (EGFR) was investigated using an IEX-1-promoter-luciferase-reporter-gene assay and blockage of EGFR by the highly specific EGFR-tyrosine kinase inhibitor PD153035. Blockage of the …
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