Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"HT1080"”.
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A tumour suppressor role for the T-box transcription factor TBX3 in fibroblasts
… as well as the naturally occurring aggressive HT1080 cell line, and the effect on key features of oncogenesis determined. In both cell lines, a substantial decrease in in vitro cell proliferation, measured using growth curve and BrdU incorporation assays, anchorage independence, measured using …
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The unfolded protein response and HLA-B27 misfolding: implications for ankylosing spondylitis
… stimuli in distinct ways. We establish that HT1080 cells inefficiently induce a UPR in response to tunicamycin and that this has consequences for cell survival. However, inefficient activation of the UPR in HT1080 cells can be overcome by secondary signals, since co-administration of the …
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Synthesis and pharmacological evaluation of novel anti-tumour prodrugs. Synthesis and pharmacological investigations into novel MMP-activated peptide-based prodrugs of methotrexate as potential cancer therapeutics
… these conjugates in tumour tissues (specifically HT1080 homogenates) ex vivo was assessed and the results were compared to the activation of these conjugates in various normal tissues specifically liver, kidney and lung. Specific criteria were established for the selection of promising conjugates …
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Ultrasound-assisted Ice Nucleation, Fibroblast Migration and Oxygen Delivery in Collagen Scaffolds for Tissue Engineering
… on cell metabolism and cell viability in HDF and HT1080 populations cultured in collagen scaffolds were investigated. Oxygen-loaded microbubbles were delivered to the centre of the cell cultures, using either 10 minutes or 30 seconds of exposure to ultrasound, daily, for 10 days. While the …
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Investigating pathways in tumour cell migration and invasion in response to novel isoquinolinone compounds
… I collagen which was paralleled in A5 treated HT1080 cells on these matrix components. Compound A5 also induced 2D migration of MDA-MB-231 cells on all matrix substrates. Cell movement of MDA-MB-231 cells through 3D matrices was modestly enhanced by A5 and significantly enhanced by A3. Using …
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A study of the expression and cellular function of the human FAM111B gene
… and upregulated in the human fibrosarcoma (HT1080) cell line by RNA-interference mediated gene silencing and recombinant gene expression technologies. The effect of these FAM111B dysregulations was studied using cellbased functional assays: proliferation, apoptosis, migration, and invasion …
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Force-extension of the Amylose Polysaccharide
… assays, using 3 different cell lines (COS-7, HT1080 and C2C12) revealed that the c.-198C>G SNP (Mut-DAF) led to an increase in DAF promoter activity (
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Investigating the functionality of candidate susceptibility genes in ophthalmoplegic myasthenia gravis using patient-derived material
… WT promoter in the human fibroblast cell line (HT1080, p=0.031) but not in a mouse myoblast cell line (C2C12, p=0.33). Next, gene expression analysis was performed in opportunistically sampled orbital fibroblasts (n=5 controls, n= 2 OP-MG) and the endogenous expression levels of ST8SIA1 was …
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세포자멸사 촉진분자를 이용한 암 치료용 단백질치료제의 개발
… 세포자멸사 촉진 효과 분석 - 19 G. Jurkat, JR-T2, HCT-116, HT1080 세포주에서의 endogenous Bax, Bcl-xL, p53 단백질 발현 양상 분석 - 20 H. wild type Bcl-xL(WT)과 돌연변이 Bcl-xL(5A)의 세포 내 위치 분석 - 22 I. wild type Bcl-xL(WT)과 돌연변이 Bcl-xL(5A,5S)의 homodimer 형성 확인 - 22 J. wild type Bcl-xL(WT)과 돌연변이 Bcl-xL(5A)의 Bax와 heterodimer 형성 확인 …
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Funktionelle Untersuchungen zum Einfluss von FAP-Inhibitoren auf die Tumorzellmigration und Invasion
… Funktion von FAP in migrierenden fibroblastoiden HT1080 Fibrosarkomzellen, die FAP ueberexprimierten. FAP war in Zellen, die durch 3D Kollagenmatrices migrieren, an Zellkontakten zu Kollagenfasern geclustert, mit ß1-Integrin kolokalisiert (Konfokalmikroskopie) und wurde im Verlauf der Kultur auf …
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MECHANISM OF TRANSCRIPTIONAL REPRESSION OF MMP-9 BY INTERFERON-β
… repression of MMP-9 transcription by IFN-beta in HT1080 fibrosarcoma cells. Using reporter assays with promoter deletion constructs we show that IFN-beta's inhibitory effects require a region of the promoter between -154 and -72, which contains an AP-1 binding site. Chromatin immunoprecipitation …
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The role of heparin and heparin-binding growth factors in pre-eclampsia
… model was performed with human fibrosarcoma HT1080 invasive cells (positive control), mouse embryonic fibroblast NIH/3T3 non-invasive cells (negative control) and immortalised human primary villous trophoblastic cell lines TCL-1.The greatest stimulation was by FGF2, PDGF-BB, HGF, MK and …
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Mechanism of Human Papillomavirus E6-Mediated Repression of P53-Target Gene *Transcription
… We used UV-irradiated human fibrosarcoma HT1080 cells treated with proteasome inhibitors (MG132) and in vitro-assembled chromatin templates reconstituted with purified chromatin assembly factors (Acf1 and ISWI), core histones (H2A, H2B, H3, and H4), and a histone chaperone (NAP-1) in order …
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Repression of telomerase components in isogenic normal, immortal and tumorigenic cells
… as well as a fibrosarcoma derived cell line, Ht1080. siRNA-mediated inhibition of dyskerin or hTERT had a potent anti-proliferative effect on immortal and tumorigenic cells, but had no overt effect on the proliferation of isogenic normal cells. Suppression of dyskerin or hTERT also impaired …
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Molecular Characterization of pFGE, the Paralog of the C-α-Formylglycine-generating Enzyme
… die Enzyme-Replacement-Therapie in Fibroblasten (HT1080) exprimiert werden. Die Ergebnisse zeigen, dass die spezifische Aktivität von Galactose-6-Sulfatase durch Koexpression von FGE bis zu 100fach gesteigert werden kann. Eine solche Steigerung setzt jedoch die gleichzeitige Expression beider …
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The role of cancer stem cells in osteosarcoma
… cell line MCF7 and a fibrosarcoma cell line (HT1080). Analysis of the cytokine expression profile of OS cell lines (HOS, MG63 and U2OS) demonstrated these cells secrete a broad range of cytokines associated with inflammation. The cytokine CCL-2 was identified as the putative OS paracrine …
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Transcriptional regulation of the human alpha 2(I) procollagen gene
… FG₀), transformed fibroblasts (CT-1, SVWI-38), HT1080 fibrosarcoma, HepG2 hepatocellular carcinoma, L77 lymphoblasts and breast cancer epithelial cells (MDA-MB-231, ZR-75-2). These differences were due to changes in transcription of the α2(1) procollagen gene as shown by Northern blot analysis …
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Investigating the functionality of African-specific variants in the TGFB1 regulatory region and their potential role in HIV-associated kidney complications
… after co-transfecting renal RCC4+VHL and HT1080 fibroblast cells with a HIV Tat expression vector and the H-6, H-5 and H-AFR promoter luciferase constructs. Results showed that the promoter activity for all TGFB1 haplotypes was upregulated in the renal cells (≥1.6-fold; p<0.001). The same …