Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 68 for “"HT-29"”.
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DNA polymerase beta inhibitor pamoic acid : toxicity to metakaryotic human cancer stem cells (HT-29)
… in the human colorectal cancer cell culture, HT- 29mes, that expresses characteristics expected of colorectal cancer metastases. We conclude that, at 300 [mu]M and above pamoic acid does not selectively kill metakaryotes in cell culture below that concentration which kills eukaryotes. Rather, …
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Apple pomace polyphenols and their effect on the proliferation of human epithelial colorectal adenocarcinoma cells (HT-29)
… epithelial colorectal adenocarcinoma cells (HT-29). Their proliferation was quantified by use of a cell-protein binding dye, sulforhodamine B.
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Intestinal Inflammatory Responses to the Indigenous Microbiota
… cells to indigenous bacteria was evaluated in HT-29 cells associated with Lactobacillus plantarum 299v in the presence or absence of tumor necrosis factor-alpha (TNF-alpha). Co-culture of TNF-alpha-stimulated HT-29 cells with L. plantarum 299v increased interleukin-8. (IL-8) mRNA expression …
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Immunomodulatory effects of lactic acid bacteria on human intestinal epithelial cells and macrophages in the context of a pro-inflammatory challenge
… lines (U-937) and intestinal epithelial cells (HT-29) induced by Tumor Necrosis Factor α was assayed by ELISA for interleukin-8 (IL-8). Granulocyte-macrophage colony stimulating factor (GM-CSF) production was assayed by ELISA in the HT-29 cell line. Strain-dependent differences were observed in …
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Targeted nanoparticle platforms for selective photodynamic therapy of cancer
… PDT treatment of cancer cells using zinc phthalocyanine (C11Pc)-PEG gold nanoparticles (ca. 4 nm). Using a number of colorimetric techniques the interactions of Concanavalin A and succinyl-Concanavalin A with MCF-7 and SK-BR-3 human breast cancer cells expressing α-mannose was explored. The …
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Protektiver Effekt von 6-Shogaol, Ellagsäure und Myrrhe auf die intestinale epitheliale Barriere
… an den intestinalen Kolonepithelzellmodellen HT-29/B6 und Caco-2 auf ihr Potential hin, die intestinale Barriere zu stärken bzw. eine Barrieredysfunktion zu verhindern, untersucht. Hauptschwerpunkt der Analysen ist die parazelluläre Barrierefunktion und die Regulation der dafür entscheidenden …
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Modulation von Zellzyklus- und Apoptoseregulation in humanen kolorektalen Karzinomzellen durch Butyrat und Acetylsalicylsäure
… es zu einer Steigerung der Differenzierung von HT-29-Kolonkarzinomzellen, jedoch nicht von Caco-2 Zellen. Acetylsalicylsäure allein hatte keinen relevanten Effekt auf die Zelldifferenzierung und in Kombination konnte Acetylsalicylsäure die differenzierende Wirkung des Butyrats nicht verstärken. …
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Characterizing induced gene expression in Shigella flexneri following bile salt exposure
… 2457T strains were used in infection of HT-29 colonic epithelial cells to compare invasion ability and intracellular replication. The Congo red (CR) secretion assay was also used as a measure of virulence protein secretion from the type-III secretion system (T3SS), while interleukin-8 …
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Investigation of checkpoint adaptation in human cancer cells treated with the genotoxic agent cisplatin
We investigated checkpoint adaptation in HT-29 colorectal adenocarcinoma cells treated with cisplatin. Cells that undergo checkpoint adaptation arrest at and then abrogate the G2/M checkpoint to enter mitosis with damaged DNA. We identified that cytotoxic amounts of cisplatin induce either …
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Inhibitory potential of anthocyanins and anthocyanin-rich extracts on colorectal cancer progression through apoptosis, angiogenesis, and immune response effects
… highly tied to lifestyle aspects: diet, body weight, and activity. Determining dietary changes to assist in preventing or slowing progression to metastasis could increase survival rates. Anthocyanins, a subcategory of flavonoids, are natural red, purple, and blue pigments in a variety of plants …
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Testing the Anti-Inflammatory Effects of Dietary Phytochemicals in Intestinal Inflammation Models
… transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-ƙB) which promotes transcription of neutrophil gelatinase associated lipocalin (NGAL), an antibacterial protein positively correlated with UC severity. Accordingly, we hypothesized that CA would upregulate …
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Regulation of tumor necrosis factor-alpha induced apoptosis via posttranslational modifications in a human colon adenocarcinoma cell line
… 200 phosphosites were identified from HT-29 cells, some of which were detected only from insulin-treated cells. Our phosphoproteomics approach also enabled us to detect alteration of both known and unknown phosphorylation states of apoptosis-related proteins at two time points during …
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Regulation of apoptosis in human cancer cells
… stimulating human colorectal cancer cells, HT-29 and HCT- 116, and human prostate cancer cells, LNCaP, with cytokines in order to induce cell death. Then, we observed the effects of NO inhibitors, kinase inhibitors, and activation of Akt, a kinase up-stream of the caspase cascade, following …
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Chemical and biological evaluation of palythoa tuberculosa collected from the red sea
… three human cancer cell lines (MCF-7, HeLa, and HT- 29). Palysterol G, in particular, was able to induce apoptosis in breast adenocarcinoma(MCF-7) cells
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Correlation between the expression of integrins and their role in cancer progression. Expression pattern of integrins αvβ3, αvβ5 and α5β1 in clinical and experimental tumour samples
… variable levels in OSC-19, PC-3, DU145, DLD-1, HT-29, HUVEC, MCF-7, MCF-7ADR and M14 human tumour cell lines and in OSC-19, PC-3, HT-29 and MCF-7 xenografts. α5β1 integrin was expressed in all cell lines and xenografts except in MCF-7 cell line and HT-29 cell line and xenograft. Overall, the …
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The role of probiotics, prebiotics, and human milk oligosaccharides in infant formulae
… infants consuming B. lactis had heightened immune response, as evidenced by increased anti-rotavirus- and anti-poliovirus-specific IgA following immunization. These results demonstrate that negative immune-related effects of not breast feeding and cesarean delivery can be mitigated by …
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Host-microbe interactions in the inflamed gut
… we performed in vitro studies using HT-29 colon cancer cells. The HT-29 cells were treated with bacterial conditioned media obtained by growing either A. muciniphila (AM-CM) or NCIMB (NCIMB-CM) or both together (COMB-CM) in Dulbecco’s Modified Eagle Medium (DMEM) for 2 h at 37 °C …
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The Structural Requirements of Gastrin and Glycine-Extended Gastrin for Binding to Receptors from Normal and Cancerous Human Tissues
… the human colon cancer cell lines DLD-1 and HT-29. Binding of CCK8 and gastrin peptides to membrane preparations and whole cells, in competition with [3H][Leul5]G17-Gly and [l0I][Leu‘5]G17-Gly, was assessed. Binding of ['HJCCK8 to DLD-1 membranes was also examined. The ability of peptides to …
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Estudio de los lncRNAs MDL1 y MDL1AS en diferentes tipos de tumores y su relevancia clínica en adenocarcinoma rectal
… Finally, in an in vivo model, using the HT-29 cell line to initiate tumor growth, we identified that the primary tumor and metastases present lower MDL1AS expression than the original HT-29 cells; however, when treating the metastases with chemotherapy, MLD1AS expression increased. …
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