Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 30 for “"HSL"”.
-
Omics Techniques Applied to Diabetes Research - Focus on HSL-Null Mice and Clonal β-Cells
… models were analyzed: hormone-sensitive lipase (HSL) null mice and a pancreatic clonal β-cell line to study the mechanisms underlying insulin resistance and insulin secretion perturbations, respectively. To achieve this, techniques allowing a global analysis of the transcriptome, proteome and …
-
Lipid metabolism in the pancreatic beta-cell. Implications for insulin secretion.
… enzyme in lipolysis: hormone sensitive lipase (HSL). To this end, both a global knock out of HSL and a beta-cell specific KO of the enzyme (beta-HSL KO) were created. In theory, disruption of HSL could cause an accumulation of lipids, inducing cellular toxicity, as well as having an effect on …
-
Hormone-Sensitive Lipase - New roles in adipose tissue biology
… combat this disease. Hormone-sensitive lipase (HSL) is a key enzyme in the mobilization of fatty acids from acylglycerols in adipocytes, but also plays important roles in other cell types. The aim of this thesis has been to study the consequences of a targeted disruption of the HSL gene in mice, …
-
N-acylhomoserine lactone regulation of adhesion and biofilm differentiation in Serratia marcescens MG1
… as N-butanoyl-L-homoserine lactone (C4-HSL). The aim of this study was to determine the role of C4-HSL in surface colonisation (adhesion and biofilm formation). In this thesis, the development of a novel biofilm in S. marcescens MG1 is described. The biofilm comprises of an intricate and …
-
Influence of parental swimming stamina on the cardiac and metabolic performance of larval zebrafish (Danio rerio).
… the two parental groups -high stamina larvae (HSL) and low stamina larvae (LSL)- were reared at 27°C in aerated water (21% O2). Routine and active heart rate, routine and active mass specific oxygen consumption were recorded through 21dpf, and cost of transport (COT) and factorial aerobic scope …
-
Investigating Microbial Quorum Sensing Potential for Enhanced Production of Biodegradable Polymers
… different exogenous quorum molecules sensing (C4-HSL, C6-HSL and 3-oxo-C12-HSL), to study if there was a change in the amount of PHA produced. It was observed that C. necator H16 increased production of PHAs, specifically small chain length PHA (SCL PHA) poly(3-hydroxybutyrate) (P(3HB)), by 13.03% …
-
The Influence of Changes In theMolecular Structure of N-Acylhomoserine Lactones On the Complexation and Dna-Binding of Luxr Protein
… N-(3-oxohexanoyl)-homoserine lactone (3OC6-HSL), during bacterial growth has made it possible to purify soluble, native LuxR from recombinant E. coli. The goal of my research was to investigate the interaction between LuxR and the lux box DNA as modulated by changes in the molecular …
-
The quorum-sensing regulation of Vibrio fischeri : novel components of the autoinducer/LuxR regulatory circuit
… signal molecules (luxI-dependent 30C6-HSL and the ainS-dependent C8-HSL) and the transcriptional activator LuxR regulate the luminescence system in a cell-density dependent manner by a process termed quorum sensing. In this study, five additional proteins whose production is regulated …
-
Insights into the Substrate Binding Specificity of Quorum-Quenching Acylase PVDQ
… that binding of C12-homoserine lactone (C12-HSL) ligand reduced the global motion of the complex and maintained the correct arrangement of the catalytic site. Further, the residue interaction network analysis of each system illustrated that there are more communication contacts and pathways …
-
Molecular and transcriptomic studies of quorum sensing in Chromobacterium subtsugae
… respond to a variety of AHLs in addition to C6-HSL. Previously, it was shown that the promiscuous activation of CviR by B. thailandensis AHLs provides a competitive advantage to C. subtsugae. Here, we show that B. thailandensis AHLs activate transcription of dozens of genes in C. subtsugae, …
-
Analysis of the Regulons Controlled by Transcriptional Regulators LuxR and LitR in Vibrio fischeri
… 3-oxo-hexanoyl-L-homoserine lactone (3-oxo-C6-HSL). A lacZ reporter was used to show that the promoters for qsrP, acfA, and ribB were directly activated via LuxR-3-oxo-C6-HSL in recombinant Escherichia coli. The sites of transcription initiation were established via primer extension analysis. …
-
Engineering plant-microbe communication for synthetic symbioses
… show that the p-coumarate homoserine lactone (pC-HSL) sensors can respond to pC-HSL biosynthesized by Pseudomonas putida grown in proximity to the plants. To the best of our knowledge, this is the first demonstration of engineered microbe-to-plant communication using a small molecule. We were also …
-
Indução do mecanismo de quorum sensing por autoindutor-1 em Salmonella enterica e prospecção de inibidores
… ausência de N-dodecanoil homoserina lactona (C12-HSL), mostraram que estes perfis foram alterados na presença do AI. Além disso, os perfis de proteínas e ácidos graxos variaram menos ao longo do tempo de cultivo na presença de C12-HSL, ou seja, células cultivadas por 4 h (fase logarítmica) e por …
-
The role of skeletal muscle PLIN proteins at rest and following lipolytic stimulation
… as well as protein interactions with ATGL and HSL at rest and following lipolytic stimulation. In addition, the serine phosphorylation state of PLIN2, PLIN3, and PLIN5 was determined at rest and following lipolytic stimulation. An isolated whole muscle technique was used to study the effects of …
-
The impact of carbohydrate ingestion on the regulation of fat oxidation following exercise
… klnase (AMPK) and hormone sensitive lipase (HSL) increased with exercise (p<0.05). ACC and AMPK phosphorylation in the CHO trial was lower at 1 h post than in the PLA trial (pc0.05). HSL phosphorylation remained above baseline at 1 h post in the PLA but not the CHO trial. Conclusion: Results …
-
Fatty Acid Fate In Determining Oxidation And Inflammation In Adipose Tissue
… by inhibition of hormone sensitive lipase (HSL). In 3T3-L1 adipocytes, limiting lipolysis potentiated the induction of oxidative genes; while in contrast, promoting the accumulation of intracellular FAs suppressed their induction by Β-AR stimulation. Interrogation of the Β-adrenergic …
-
Machine learning approach for high speed link modeling and IBIS-AMI model generation
… This work reports modeling the high-speed link (HSL) system using machine learning and implementing the model into IBIS-AMI, an industrial standard for SerDes simulation and verification. We started with developing a Volterra series model by extracting the Volterra kernels using feed forward …
-
Formalization Of Input And Output In Modern Operating Systems: The Hadley Model
… We present test results from operation of our HSL-driven system both in user mode on stored disk images and as part of a Linux kernel module allowing file systems to be read. We conclude by discussing future directions for the research.
-
Effective Features and Machine Learning Methods for Human Recognition Based on Multi-biometric Systems
… by using the brightness channel in HSV And HSL color spaces. Distance similarity measures have been compared for PCA- and LDA-based face, ear and palm biometrics. The face and ear recognition performance using SVM based on PCA and SVM based on a combination of PCA and LDA techniques have …
-
Genetic Analysis of the Quorum Sensing Regulator EsaR
… occurs after it complexes to the AHL (3-oxo-C6-HSL), unlike the LuxI/R system, this activation occurs by a different mechanism. At low cell densities, dimerized EsaR acts as a repressor. At a high cell population, derepression of the EPS genes occurs via an unknown mechanism once the AHL …
Page 1 of 2