Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"HPRT"”.
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Kinetic and metabolic studies in HPRT deficiency
… This thesis examines the patient and the variant HPRT at three levels. Firstly, a detailed and comprehensive study of the kinetic properties of the variant enzyme was made. The novel feature of the kinetics is the presence of substrate inhibition by the purine bases, with a true Kᵢ value for …
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Biochemical and genetic properties of HPRT Cape Town
An unusual partial HPRT deficient mutant, HPRT Cape Town was observed to have a low activity in erythrocyte lysates at high concentrations of the purine substrates, hypoxanthine and guanine. This substrate inhibition was not observed with the substrate PPRP. The low activity was not associated with …
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The spontaneous mutational spectrum of exon 2 and the high melting region of exon 3 of the human HPRT gene
Thesis (S.M.)--Massachusetts Institute of Technology, Division of Bioengineering and Environmental Health, 1999.
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The expression and drug targeting of parasitic hypoxanthine-guanine phosphoribosyltransferase (HGPRT)
… hypoxanthine-guanine phosphoribosyltransferase (HPRT) in E. coli using the pET expression system. The cDNA encoding the ORF of HPRT was amplified by PCR and transformed into E. coli cells using standard methods. Expression was induced by IPTG and reached about 13% of the total cell protein for …
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The biochemical and molecular basis of Hypoxanthine-guanine phosphoribosyltransferase deficiency
Hypoxanthine-guanine phosphoribosyltransferase (HPRT) catalyses the first step in purine salvage. A complete deficiency of the enzyme results in the devastating neurological symptoms of the Lesch-Nyhan syndrome. The Lesch-Nyhan syndrome is characterised by purine overproduction leading to, …
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The role of Xist RNA in the maintenance of X chromosome inactivation
… of two X-linked genes, the endogenous Hprt gene and an X-linked GFP transgene, can be quantitatively assessed. Mouse embryonic fibroblasts derived from mice carrying the conditional Xist mutation were cultured and infected with an adenovirus vector carrying the gene for Cre recombinase. …
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Zyto- bzw. gentoxische Wirkschwellen von Gemischen aus 2 - 8 Umweltchemikalien in Abhängigkeit von der Lipophilität der Komponenten
… von Gemischen aus MMS und Pentachlorphenol im HPRT-Mutagenitätstest in V79 Zellen. Substanzgemische der Chemikalien, deren einzelne Komponenten in subtoxischen Konzentrationen eingesetzt wurden, verursachten synergistische, zytotoxische Kombinationswirkungen. Die Toxizität der Gemische nahm mit …
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Purification and characterisation of plasmodium falciparum Hypoxanthine phosphoribosyltransferase
… focuses on improving the purification of PfHPRT by investigating the characteristics of anion exchange DE-52 chromatography (the first stage of purification), developing an HPLC gel filtration method for examining the quaternary structure of the protein and possible end stage purification, …
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Stabile Expression von Sulfotransferasen - allein oder in Kombination mit Cytochrom P450 - in Zelllinien für Mutagenitätsuntersuchungen
… 2,4-Diaminotoluol) auf ihre Mutagenität am hprt-Locus hin untersucht.Die aAA waren in Zellen, die keine rekombinanten Enzyme oder lediglich CYP1A2 exprimierten, nicht mutagen. In Zellen, die CYP1A2 und SULT der Subfamilie 1A koexprimierten, erzeugten sie bereits in geringen Konzentrationen …
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Proceso apoptótico y respuesta simpática en tejido cardiaco durante la muerte encefálica
… ACTB, YWHAZ, ARN 18S, B2M, UBC, TBP, RPLP y HPRT) para ser usados como genes de referencia para la normalización de datos obtenidos en estudios de expresión génica en tejido humano procedente de donantes en muerte encefálica. Los principales resultados obtenidos fueron: - Los genes PPIA, RPLP …
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A clamp ligation method for point mutational spectrometry : marked increase in scanning range for the human genome
… hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene (cDNA-bp 223-318), was analyzed using both the natural and ligated clamps. A sensitivity of 2 x 10-5 in human cells was demonstrated using the ligated clamp as opposed to 5 x 10-6 using the natural clamp.
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Point mutations in normal lungs of smokers and non-smokers
… in TP53 bp742, bp746 and bp747, K-ras bp35 and HPRT bp508 from a total of 291 tracheal-bronchial epithelial sectors from six smokers and nine non-smokers, yielding 949 individual mutational assays. The conditions of MAMA for each target point mutations were optimized such that the sensitivity of …
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Differentiation and contractility of colon smooth muscle under normal and diabetic conditions
… hypoxanthine-guanine phosphoribosyltransferase (Hprt)-targeted reporter transgenes revealed that a 10 base pair large CC(AT)₆GG ciselement, called CArG box is required for promoter activity in all tissues. We also determined that an additional 100 base pair region is necessary for transgene …
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An evaluation of cancer biomarkers in normal ovarian epithelial cells and ovarian cancer cell lines
… against normal housekeeping genes GAPDH and HPRT. In addition, immunocytochemistry was also used in the validation process of the aforementioned genes. Significance: ovarian cancer cells express gene signatures, which pose significant challenges for cancer drug development, therapeutics, …
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REGULATION OF THE HUMAN NEUROTROPIC POLYOMAVIRUS, JCV, IN THE CENTRAL NERVOUS SYSTEM
… of primary human fetal astrocytes (PHFA) with an HPRT-deficient glioblastoma cell line, U-87MG. The new hybrid system was then used to analyze the ability of JCV replication and gene expression by infection studies. Results demonstrated that the new hybrid lines efficiently support JCV propagation …
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The effects of ethanol and aspalathus linearis on immortalized mouse brain endothelial cells (bEnd5)
… normalized against housekeeping genes, GAPDH and HPRT. Transepithelial electrical resistance (TEER) was measured using the Ohm Millicell-electrical resistance system, while bEnd5 monolayer morphology was analysed using the Zeiss scanning electron microscope. Both concentrations of EtOH led to an …
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Biological and Functional Consequences of Single Nucleotide Polymorphisms of the O6-Methylguanine-DNA-Methyltransferase Gene
Single nucleotide polymorphisms (SNPs) in DNA repair genes could alter the transcriptional levels, structure and function of DNA repair proteins and alter DNA repair proficiency. Consequently, these SNPs could significantly influence the level of genetic damage, which is an early critical factor in …
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Charakterisierung von Sulfotransferasen im Gastrointestinaltrakt von Mensch und Ratte und Aktivierung von Promutagenen in V79-Zellen, die eine intestinale Form (1B1) des Menschen und der Ratte exprimieren
… standen 1B1-profiziente Indikatorzellen für den HPRT-Genmutationstest zur Verfügung, und die 1B1-abhängige Bioaktivierung konnte in einem System untersucht werden, die dem eukaryontischen Organismus näher steht als die für die Ames-Tests verwendeten Bakterien. So war z.B. die Sulfotransferase wie …