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Showing 1 to 15 of 15 for “"HPLC assay"”.
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The determination of drug stability by HPLC assay of degradation products.
… decomposition studies on several drugs. HPLC with ultraviolet detection is used as a single analytical method for both reactant and product. Aspirin and diiodoaspirin represent single-decomposition product systems; tetracycline is examined as a drug decomposition involving parallel, …
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Prolactin Effects on Nigrostriatal and Mesolimbic Dopaminergic Neurons: In Vivo and In Vitro Studies
… and in vitro superfusion techniques coupled to HPLC assay, the effect of prolactin on nigrostriatal and mesolimbic dopaminergic neuronal activity and its possible regulatory mechanism were examined.
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HPLC analysis of Romet-30 in chinook salmon (Oncorhynchus tshawytscha): wash-out time, tissue distribution in muscle and liver tissues, and metabolism of sulfadimethoxine
… concern to the consumer's health, analytical assay is required to monitor the antimicrobial residue levels in edible fish tissue. The focus of this research was to identify a "marker" tissue which would have a higher residue level than the muscle tissue, and that the ratio between this …
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Comparison of the sutherlandioside B levels in two commercially available Sutherlandia frutescence preparations and the effect of elevated temperature and humidity on these levels
… to develop a validated stability-indicating HPLC assay for sutherlandioside B (SU-B); secondly, to compare the SU-B levels in the two commercially available Sutherlandia products viz, the Phyto Nova Sutherlandia SU1™ tablet and the Promune™ capsule, and, thirdly, to determine the effect of …
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Molecular Studies to Investigate 4-hydroxy-2-oxoglutarate Metabolism Defects of Primary Hyperoxaluria Type 3
… consistently beyond 8 Å. Additionally, a new HPLC assay was developed for monitoring HOGA activity in crude lysates. Results from this assay indicated that exposure to the chemical chaperones TMAO, glycerol, and DMSO during expression in E. coli did not increase the activity of several HOGA …
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Development of a HPLC method for the determination of methotrexate (MTX) and MTX-polyglutamates of red blood cells: A dissertation submitted in partial fulfilment of the requirements for the Degree of Bachelor of Science with Honours at Lincoln University
… rapid high-performance liquid chromatographic (HPLC) assay to determine MTX and MTX(glu)s in RBC is described. After extraction of MTX(glu)s from RBCs, chromatographic separation was achieved using a C₁₈ reversed-phase column, followed by ultraviolet (UV) detection at 254 nm. RBC standards for …
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Development of a topical epigallocatechin gallate (EGCG) formulation
… A high-performance liquid chromatography (HPLC) assay was developed to evaluate the possible degradation of EGCG from the various solvents used to incorporate it into topical formulations in the preformulation studies. This method was validated to establish linearity, precision, and …
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Investigation of ciprofloxacin antibacterial combinations.
… of P. aeruginosa and S. aureus. Two separate HPLC methods were developed to obtain a separation between ciprofloxacin and sulphadiazine, and between ciprofloxacin and trimethoprim in Iso-sensitest broth. Each method was capable of quantitating each compound within the microbiological matrix …
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Analysis of the Metabolism of Dextran-Methylprednisolone Succinate Using
… @exMPS). A newly developed reversed-phase HPLC assay is validated, and is capable of simultaneous measurement of methylprednisolone (MP), methylprednisolone succinate (MPS), methylprednisone (MPN), and corticosterone (CST) in addition to an internal standard (IS), triamcinolone acetonide. …
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Transport and utilization of arginine and arginine-containing peptides by rat alveolar macrophages
… for nitric oxide (NO) production.;Method. A HPLC assay was developed for quantitative measurement of Arg and ACPs in rat plasma and bronchoalveolar lavage (BAL) fluid. The uptake of small peptides by rat AM was evaluated using fluorescein isothiocynate (FITC)-labeled (*) peptides (Arg-Lys*, …
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A 'Biorelevant' Approach for Accelerated In Vitro Release and In Vitro-In Vivo Relationship of a Biodegradable, Naltrexone Implant
… High Performance Liquid Chromatography (HPLC) assay. A miniature, cell-culture, capillary system was modified and tested as a 'biorelevant' alternative to the modified flow-through apparatus, to mimic significant barriers to drug release that would be expected in vivo. The in vitro …
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Pharmaceutical applications and crystal structures of trehalose and its derivatives.
… and proposed excipients. A stability indicating HPLC assay (using an ODS C8 column), to quantify ascorbic acid recovery from tablets containing trehalose, has been developed, validated, and applied in the context of the tablet matrix. Accelerated storage testing has been undertaken for ascorbic …
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Preformulation and Formulation Development of a Novel Radioprotectant Drug
… levels of radiation. A rapid and sensitive HPLC method was developed and validated for the determination of 1210. An isocratic system using a C-18 Gemini column (250 x 4.6 mm) with column effluents monitored at 254 nm was used. The mobile phase comprised of acetonitrile:0.1% trifluroacetic …
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Development of amoxicillin suppositories and in vitro/ in vivo evaluations
… high-performance liquid chromatographic (HPLC) method was developed and applied to validate the extraction protocol developed by leveraging the solubility and stability of amoxicillin. The developed HPLC method was then used for in vitro and in vivo quantification of amoxicillin. …
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Development and characterization of biodegradable microspheres containing selected antimycobacterials.
… were required. Stability indicating HPLC assays to quantify drug release have been developed, validated and applied to monitor drug release based on cumulative quantification of drug and degradates. A mathematical correction for serial decompositions associated with RIF was made based …