Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"HPLC/ESI-MS"”.
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Untersuchungen zur Proteolyse von para-k-Casein: vom Modell zum Käse
… Methoden (SDS-Elektrophorese, IEF, GPC, RP-HPLC, ESI-MS u.a.) konnte der Abbau des para-k-Caseins durch die industriell relevanten Milchgerinnungsenzyme Chymosin, Fromase und Suparen bei unterschiedlichem Wasserangebot verfolgt werden. Para-k-Casein wird bei einem käseüblichen Wassergehalt …
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Elicitoren der Duftstoffemission von Rainfarnpflanzen
… sich mit der Suche nach Elicitoren im Vorderdarmsekret (Regurgitat) von Spodoptera littoralis - Raupen für die Auslösung der Duftstoffproduktion in Rainfarnpflanzen. Vier HPLC-Fraktionen F0/2, F0/4, F0/5 und F0/6 der durch Festphasenextraktion aus dem Regurgitat von Spodoptera littoralis - …
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Tracing viable bacteria in Wadden Sea sediments using phospholipid analysis
This thesis describes a protocol that was developed to analyse different types of lipids from bacteria and sediments. Special emphasis was placed on the analysis of intact phospholipids by HPLC-ESI-MS/MS, because phospholipids appear to be suitable biomarkers to trace viable bacteria. The …
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Biological Consequences Of Complex Dna Lesions Induced By Bis-Electrophiles
… of DNA adducts. In the first part of this thesis, we investigated the ability of human cells to repair three potentially mutagenic BD–adenine lesions: N 6–(2–hydroxy–3–buten–1–yl)–2 ’–deoxyadenosine, 1,N 6–(2–hydroxy–3–hydroxymethylpropan–1,3–diyl)–2 ’–deoxyadenosine and N 6–,N …
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The role of PTEN as a PI(3,4)P2 lipid phosphatase in Class I phosphoinositide 3-kinase signalling
… the work performed to develop a novel, HPLC-ESI MS/MS based method, in order to measure the product of PI(3,4,5)P3 5-dephosphorylation, PI(3,4)P2, separated from its more abundant regioisomer in cells - PI(4,5)P2. This and an existing HPLC-ESI MS/MS method for measuring PI(3,4,5)P3, have …
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Caratterizzazione proteomica di fluidi e tessuti in diverse condizioni fisio-patologiche
This thesis has been focused on the proteomic characterization of human saliva from donors of different ages, starting from birth up to adult age, and pediatric brain tumor tissues. The first study has been performed in order to compare the acid-insoluble fraction of saliva from preterm with …
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Isotopic labeling of isoflavones from plant cell and organ culture for metabolic tracking in animal models
… and feces. Femur bone extracts were analyzed by HPLC-ESI-MS and found to contain puerarin, daidzein, and puerarin glucuronide, which suggests that kudzu isoflavones may be able to have a role in promoting bone health. To improve kudzu isoflavone yields in vitro for further studies, kudzu hairy …
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The remineralization of marine organic matter by diverse biological and abiotic processes
… processes — including mechanical mechanisms such as dissolution and disaggregation of sinking particles, and abiotic processes such as photooxidation — remain poorly constrained. In this thesis, I examine the biogeochemical significance of various alternative pathways of organic matter …
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Identification and Quantification using HPLC-ES/MS of Four Pentacyclic Oxindole Alkaloids in Uncaria tomentosa Wild Type
… of metabolites in the roots, leaves, and stems of U. tomentosa by establishing a method to accurately quantitate the concentrations of metabolites. In particular, we are interested in identifying and quantifying four principal pharmacologically active pentacyclic oxindole alkaloids: …
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Effects of Smoking and Gender on Tetrahydroisoquinolines and Beta-Carbolines in a Healthy Population and During Alcohol Detoxification
… To support the clinical investigations, a HPLC-FD method was developed and validated to assess plasma concentrations of BCs, harman and norharman, while a HPLC-ESI-MS/MS method was validated to quantify the TIQs, R/S-salsolinol along with dopamine. Forty-one young volunteers were recruited …
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The remineralization of marine organic matter by diverse biological and abiotic processes
… processes - including mechanical mechanisms such as dissolution and disaggregation of sinking particles, and abiotic processes such as photooxidation - remain poorly constrained. In this thesis, I examine the biogeochemical significance of various alternative pathways of organic matter …
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Monomeric Ellagitannins in Oaks and Sweetgum
… of both the reaction conditions and the gradient HPLC analysis has led to the development of a robust and reliable protocol. The chemical stability of the two predominant ellagitannins in oaks (vescalagin and castalagin) were evaluated in aqueous methanol and water. It was found that oxygen, pH …
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Untersuchungen zur endogenen Bildung, Cytotoxizität und DNA-schädigenden Wirkung des dopaminergen Neurotoxins TaClo
… (1-Methyl-4-phenyl-1,2,3,5-tetrahydropyridin) besitzen, werden als Hauptursachen für die Entstehung des Parkinsonsyndroms diskutiert. Diese Arbeit beschäftigt sich mit einer neuen Klasse von neurotoxisch wirksamen Tetrahydro-b-carbolinen, die sich von Trichloracetaldehyd (Chloral) ableiten. Die …
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Probing the redox-active residues in cytochrome c peroxidase
… CCP [CCP(MI)], HRP and their CN-ligated forms were investigated to probe the structural basis of peroxidase polypeptide vs heme reactivity. Despite similar structures, the kinetic stabilities and conformational states of CCP(MI) and HRP were found to be significantly different. The role of …
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Ruolo dell'Enantiomero (R)-9-HSA nel controllo della proliferazione in una linea di Adenocarcinoma del Colon umano
… in this study, (R)- and (S)-9-HSA were synthesized and their biological activity tested in HT29 cells. At the concentration of 50 M (R)-9-HSA showed a stronger antiproliferative effect than the (S) isomer, as indicated by the growth arrest in G0/G1. The inhibitory effect of (S)-9-HSA on …
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Exploitation of agricultural and forestry waste: from the recovery of bioactive compounds for nutraceutical applications to the production of innovative materials
… and other innovative materials. In this thesis, dry fruit shells have been employed as sources of valuable bioactive compounds. Specifically, the Response Surface Methodology (RSM) coupled with Box-Behnken design (BBD) models were adopted to optimize entirely eco-sustainable …
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Exploitation of agricultural and food wastes: from the recovery of bioactive compounds for nutraceutical applications to the production of innovative materials [Valorizzazione di scarti agricoli ed alimentari: dal recupero di composti bioattivi per applicazioni nutraceutiche alla produzione di materiali innovativi]
… e altri materiali innovativi. In questa tesi, i gusci di frutta secca sono stati impiegati come fonti di preziosi composti bioattivi. In particolare, la metodologia della Superficie di Risposta (RSM) accoppiata a modelli di progettazione Box-Behnken (BBD) sono stati utilizzati per …
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Sensitivity to chemical stimuli plays a fundamental role in the food preferences. Examples in the evolutionary scale: 1. Role of the walking leg chemoreceptors in the red swamp crayfish Procambarus Clarkii 2. PROP bitter taste sensitivity and its nutritional implications in Humans
In this thesis, we studied two examples of the sensitivity to chemical stimuli and its role in the food preferences in two models of the evolutionary scale. The red swamp crayfish Procambarus clarkii (Girard, 1852) (Crustacea: Decapoda) is an invasive species of freshwater habitats that has spread …
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Analysis of organosilicone surfactants and their degradation products
… Pure oligomers (n = 3, 6 and 9) were synthesised by reaction of the corresponding allyl-capped oligoethoxylate monomethyl ether M₂Dᴴ over a Pt catalyst. The allyl-capped ethoxylate monomethyl ethers were synthesised by reaction of allyl chloride with the corresponding ethoxylate monomethyl …
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