Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"HPAEC-PAD"”.
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Physicochemical studies of a novel adjuvant and conjugate vaccines
… with pulsed amperometric detection (HPAEC-PAD) were investigated for saccharide quantification in Mn A-TT vaccines. The HPAEC-PAD method for the monomer (mannosamine-6-phosphate from acid hydrolysis) permitted higher specificity and sensitivity for the free saccharide analysis …
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Stratégie pour l’analyse qualitative et quantitative de sirops de céréales enrichis par voie enzymatique en isomaltooligosaccharides
… couplée à la Détection à Amperometrie Pulsée (HPAEC-PAD), une séparation hautement sélective et une détection très sensible. Elle est une des seules méthode capable de séparer des molécules homologues ayant comme seules différences leur DP ou de leur type et la séquence de leurs liaisons. …
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ENZYMATIC PRODUCTION OF PURIFIED ISOMALTOOLIGOSACCHARIDES PREBIOTIC PREPARATIONS AND THEIR FULL QUALITATIVE AND QUANTITATIVE CHARACTERIZATION
… been achieved yet. In the present thesis, an HPAEC-PAD method has been set up in order to quantify the IMO present in syrups. Moreover, this step-forward analytical method permitted us to point out the presence of unknown IMO. Two structural determination methods were then set up and applied. …
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Characterisation of galactinol synthase II (XvGolSII) from the resurrection plant Xerophyta viscosa (Baker)
… synthase activity via an activity assay using HPAEC-PAD, where it produced galactinol from the substrates myo-inositol and UDPgalactose. Subcellular localisation examination, using an XvGolSII-YFP fusion protein, indicated localisation to the cell membrane of onion epidermal cells. Quantitative …
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Identification and characterization of an enterocyte receptor for group A porcine rotavirus
… GMX I and GMX II by acid hydrolysis followed by HPAEC-PAD revealed that these gangliosides contain glucose and galactose and either N-acetylneuraminic acid or N-glycolylneuraminic acid in equal molar ratios. These results suggest that GMX I is NeuAc-GM3 and GMX II is NeuGc-GM3. Analysis of the …
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Raffinose family oligosaccharides (RFO) biosynthesis in chickpea (Cicer arietinum L.) seeds
… objective. For the first objective, a modified HPAEC-PAD (High performance anion exchange chromatography with pulsed amperometric detector) based gradient approach was optimized to study the concentration and composition of soluble sugars in chickpea seeds. The optimized method separated all the …
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Production et caractérisation d'hydrolysats de pectine obtenus par voies enzymatique et thermique(micro-onde)
L’objectif de cette thèse est de contribuer à étudier d’une part les voies de production des oligosaccharides pectiques (POS) et d’autre part à déterminer leurs principales propriétés physico-chimiques. Pour ce faire, deux voies de production ont été utilisées : enzymatique et thermique. En ce qui …
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Recombinant production and regioselectivity study of lytic polysaccharide monooxygenases
Our dependence of the oil industry is one of today’s major concerns worldwide. Plant biomass has the potential to replace a large fraction of fossil resources for the production of fuels, chemicals and materials. Lignocellulose is, however, very recalcitrant towards enzymatic degradation, …