Global ETD Search
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Showing 1 to 20 of 22 for “"HP1"”.
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LIA4 and HP1-like Proteins Facilitate DNA Elimination During Tetrahymena Macronuclear Development
<p>As part of its sexual development, the ciliate Tetrahymena thermophila establishes a differentiated somatic genome called the macronucleus. A key aspect of this differentiation is the genome-wide removal of transposon-like and other repetitive elements. This process, referred to DNA …
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Regulation of the Heterochromatin Protein 1 by Phosphorylation of Histone H3 and the HP1 Hinge Domain
… effector proteins, Heterochromatin Protein 1 (HP1), plays important roles in heterochromatin formation. It is recruited to chromatin by interaction with methylated lysine 9 of histone H3 (H3K9me). However, it has remained enigmatic how HP1 reversibly dissociates from chromatin during mitosis, …
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Functional characterization of poly(ADP-ribose) dependent ubiquitin E3 ligase in the DNA damage response
… critical for RNF4 mediated DSB repair 51 Part B. HP1α, a pADPr dependent substrate of RNF4, regulates homologous recombination 55 1. Systematic screening of pADPr dependent substrate of RNF4 55 2. HP1α is ubiquitinated by RNF4 61 3. HP1α is pADPr binding protein 61 4. RNF4 …
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The synthetic multivulva genes and their suppressors regulate opposing cell fates through chromatin remodeling
… complex and heterochromatin protein 1 (HP1). In addition to a NuRD-like complex, which deacetylates lysine nine of histone H3 (H3K9), and HP1, we found two histone methyltransferase (HMT) genes (met-1 and met-2) that act as class B synMuv genes and repress lin-3 transcription. met-1 …
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Regulation of Chromosome Segregation by the Chromosomal Passenger Complex
… in the chromosomal dissociation of HP1 (heterochromatin protein 1) in M-phase. The phosphorylation of H3S10 by Aurora B ejects HP1 from its stably methylated histone H3 lysine 9 (H3K9) chromatin binding site, clearly demonstrating the existence of a "methyl/phos switch." While the …
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Maximum Entropy Optimization: a General Approach to Study Ordered and Disordered Proteins Reveals Key Features of Protein Phase Separation
… applied MOFF to study the phase behavior of HP1, an essential protein for posttranslational modification and spatial organization of chromatin. The force field successfully resolved the structural difference of two HP1 homologs, despite their high sequence similarity. We carried out large …
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Maintenance of Genomic Integrity by RSF1 Chromatin Remodeler
… G. RSF1 directly interacts with HDAC1 but, not HP1α during mitosis 50 H. RSF1 plays a crucial role in HDAC1 deposition at the mitotic chromosome arms 53 I. Knockdown of RSF1 causes defects in sister-chromatid cohesion and kinetochore protein assembly 56 J. C-terminal of RSF1 is necessary …
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Characterisation of HP1γ in mammalian cells
… DNA-dependent biological pathways. The mammalian HP1 (Heterochromatin protein 1) protein family consists of three members: HP1α, β and γ. Each paralogue regulates formation and maintenance of heterochromatin by binding to the repressive chromatin marks H3K9me2/3 with their chromodomains (CDs). …
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Intrinsically Disordered Proteins within the Genome
… histones (H1) and heterochromatin protein (HP1). The carboxy-terminal domain of H1 when within the nucleosome, adopts a compact but unstructured conformation that allows its positioning between the two linker DNA strands. In contrast, the amino-terminal domain of H1 undergoes a …
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Dynamic Simulation of active/inactive Chromatin Domains
… that basically every condensing agent (HMG/SAR, HP1, cohesin, condensin, DNA-DNA interaction...) can be modelled as an effective attractive potential of specific chain segments. This way the formation of individual 1Mbp sized rosettes from a linear chain could be observed. Furthermore several Mbp …
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Molekulare und funktionelle Analyse von Windei (CG12340) als Bindungspartner der Histonmethyltransferase Eggless während der Oogenese von <i>Drosophila</i>
… die Rekrutierung von Heterochromatin Protein 1 (HP1). Eggless (Egg), das Ortholog der humanen Methyltransferase SETDB1, ist für die H3K9-Trimethylierung in der weiblichen Keimbahn von Drosophila notwendig. Hier zeigen wir, dass Windei (Wde), das Drosophila-Homolog des murinen mAM und des humanen …
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Cloning, Expression and Purification of the Different Human Haptoglobin Chains and Initial Characterization by Mass Spectrometry
… in humans and presents in two allelic forms: Hp1 and Hp2. The two forms vary by the identity of their light chains: L1 or L2 for Hp1 and Hp2, respectively. Hp is most well known for its function in rapidly binding to free hemoglobin (Hb), which is subsequently removed from circulation, making …
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Methodology development for imaging histone modifications and for site-specific protein labeling in vitro and on the surface of living cells
… tau) or a methyllysine recognition domain (HP1 or Pc chromodomain), a peptide whose sequence corresponds to the Nterminus of histone H3, and a yellow fluorescent protein. The histone phosphorylation reporter exhibited a 25 % YFP/CFP emission ratio increase upon phosphorylation in vitro by …
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Wechselwirkungen zwischen Replikationsproteinen und Origin-DNA während Proliferation und terminaler Differenzierung
… von ORC1-5, CDC6, CDT1, MCM3, MCM6, CDC45 und HP1 an Fragment B nachgewiesen werden. Während der terminalen Differenzierung werden ORC1, CDC6, CDT1 und CDC45 von der preRC-Bindungsstelle entfernt, ORC2-5, MCM3, MCM6 und HP1 bleiben an Fragment B gebunden. Die Bindung von preRC-Proteinen an …
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Energy Landscaping - On the Relationship between Functionality and Sequence Mutations for Multifunctional Biomolecules
… to changes in binding affinities. Finally, the HP1 loop of RNA 7SK is analysed, revealing that the principles established for the energy landscapes of proteins extend to nucleic acids. Overall, the results indicate that sequences have evolved to exhibit the minimum number of funnels on the …
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Relationships between chromatin features and genome regulation
… that a team of heterochromatin factors - HPL-2/HP1, LIN-13, LIN-61, LET-418/Mi-2, and H3K9me2 histone methyltransferase MET-2/SETDB1 - collaborates with piRNA and nuclear RNAi pathways to silence repetitive elements and protect the germline. I also found that the TACBGTA motif is particularly …
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Characterising the interaction between TRIM28 and members of the Krab-ZFP family in transcription silencing
… 1), the heterochromatin protein 1 (HP1) protein family, and the nucleosome remodelling and deacetylase (NuRD) complex. Although many protein partners involved in silencing have been identified, the molecular basis of the protein interactions that mediate silencing remains largely …
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Identifizierung und Charakterisierung von Histonmethyltransferasen in Drosophila melanogaster
… wie Polycomb und Heterochromatin-Protein 1 (HP1), verhindert wird. Außerdem konnte eine Korrelation zwi-schen der epigenetischen ASH1-abhängigen Aktivierung der Ultrabithorax-Transkription in Drosophila mit ASH1-vermittelter Histonmethylierung und Rekrutierung oder Stabilisierung der Bindung …
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Environmental Performance Evaluation of Heating and Cooling Between Sustainable and Conventional Office Building
… this basis, two hypotheses (HP) were tested: HP1: Sustainable buildings remain sustainable in the long run. HP2: Current indicators fulfil the role for determining long term sustainability. From the sustainability point of view, this study focused only on the environmental aspect. The word …
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Dissecting the role of CHD4 in the NuRD and ChAHP complexes
… B2 SINEs and all three proteins-CHD4, ADNP and HP1-are required for restricting chromatin accessibility at B2 SINEs. A novel role for ADNP in maintaining enhancer accessibility by recruiting CHD4 at enhancer regions was discovered. Simultaneous presence of NuRD and ChAHP at these enhancer …
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