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Showing 1 to 10 of 10 for “"HOXB4"”.
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Examining the effect of CBP on the E2A-PBX1 and HOXB4 interaction
… that this ternary complex involving E2A-PBX1, HOXB4 (chosen as a representative member of the HOX family), and CBP does form. This direct interaction appears to reduce transcriptional activation by E2A-PBX1/HOXB4 heterodimers from PBX1/HOX enhancer elements. I also show that this suppression of …
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Downregulation of Prdm16 Is Critical for HOXB4-mediated Benign HSC Expansion In Vivo
<p>Overexpression of HOXB4 in hematopoietic stem cells (HSCs) leads to increased self-renewal without causing hematopoietic malignancies in transplanted mice. The molecular basis of HOXB4-mediated benign HSC expansion in vivo is not well understood. To gain further insight into the molecular events …
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Effects of HOXB4 downstream targets on the haemopoietic differentiation of pluripotent stem cells
… of HSC specification remain unclear. Enforced HOXB4 expression can enhance haemopoietic differentiation of mouse and human ESCs and generate reconstituting HSCs from mouse ESCs. We have previously shown that HOXB4 can enhance haemopoietic differentiation of mouse ESCs in a paracrine manner. …
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Identification of the Downstream Effector Genes Involved in HOXB4-Induced Expansion of Hematopoietic Progenitor Cells
<p>Overexpression of HOXB4, a member of Homeobox transcription factor family, promotes expansion of hematopoietic stem and progenitor cells both<em>in vivo </em>and <em>in vitro</em>.<em> </em>However, the molecular mechanisms underlying this effect are not well understood. In order to identify …
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Analyse des Differenzierungspotentials muriner hämatopoetischer und neuraler Stammzellen nach Modifikation ihrer Genexpression
… Stammzellen wurde der Transkriptionsfaktor HOXB4, die hämatopoetische Vorläuferkinase 1 (HPK1) oder eine Kinase-inaktive Mutante der HPK1 (HPK1M46) durch retrovirale Transduktion überexprimiert. Der Transfer wildtypischer HPK1 oder HPK1M46 Gene in Knochenmarkzellen hatte in vitro keinen …
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Functional Study of Hemogen Knockout Mouse Model
… Meanwhile, Hemgn has been found to contribute to Hoxb4 mediated hematopoietic stem cell expansion. Based on these postulations and evidences, a Hemgn knockout mouse model has been generated to study its function in normal and stress hematopoiesis. I confirmed the Hemgn expression in hematopoietic …
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Role of Polycomb Group Protein Mel18 in Hematopoietic Stem Cell Maintenance
… and ex vivo expansion such as homeobox gene Hoxb4. CUT&RUN sequencing assays revealed that loss of Mel18 reduces the H2AK119ub1 enrichment at the promoter regions of Cdk4 and Cdk6, leading to their enhanced expression in HSPCs. Furthermore, I identified a Mel18-specific chromatin loop at the …
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MULTI-OMICS APPROACHES TO CHARACTERIZE PATIENT-DERIVED SPINAL CORD ORGANOIDS AND ASSESS NOVEL GENES ASSOCIATED WITH C9ORF72-AMYOTROPHIC LATERAL SCLEROSIS
… particularly DCX, MAP2, PAX6, OLIG2, GFAP, and HOXB4. Time course quantification of precursors (SOX2 and ISL1), as well as the progressive increase of post-mitotic MNs (TUJ1 and SMI32) supported proper organoids maturation. This outcome highlights SCOs potential as a tool to recapitulate SC …
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Synthesising executable gene regulatory networks in haematopoiesis from single-cell gene expression data
… validated experimentally, demonstrating that HoxB4 and Sox17 directly regulate the haematopoietic factor Erg, and that Sox7 blocks primitive erythroid development. A general-purpose graphical tool was then developed based on this algorithm, which can be used by biological researchers as new …
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Analyses of hematopoiesis and lineage commitment from ES and iPS cells
… ES-Zellen mit dem chromatinremodellierenden HOXB4-Gen ermöglichte eine proliferative in vitro-Expansion der hämatopoietischen Vorläuferzellen. Deren Langzeit-Repopulationsvermögen in Knochenmarkstransplantations-ähnlichen Versuchen war nur 2- bis 10-fach geringer als die einer vergleichbaren …