Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"HOLO"”.
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A data processing subsystem for the Holo-Chidi video concentrator card
Thesis (M.Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2001.
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Atomic Force Microscope Conductivity Measurements of Single Ferritin Molecules
… molecules. The average current measured for holo HoSF was 13 and 5 times larger than that measured for apo HoSF as measured by c-AFM at 1V and gold ball at 2V and respectively, which indicates that the core of ferritin is more conductive than the protein shell and that conduction through the …
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General Transcription Factors Play Dual Roles In Initiation And Termination
… and the CF1 complex in yeast cells to form a holo-TFIIB complex. This complex was resistant to MNase digestion and brief exposure to high salt. The sedimentation coefficient of the holo-TFIIB complex was intermediate between that of TFIIH and TFIID. Initiation factors which remain bound on a …
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Purification of Vitamin D Binding Protein and Its Role in the Uptake of 25-Hydroxyvitamin D by Cultured Kidney Cells (Mdck Cells, Tissue Culture, Dbp)
… initial purification in a one-step procedure. Holo- and apo-DBP did not co-chromatograph upon chromatofocusing, which was used to separate the two forms. Holo-DBP was used as the source of 25(OH)D(,3) to study its uptake by kidney cells.
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STRUCTURE-BASED COMPUTATIONAL MODELING OF PROTEIN-LIGAND INTERACTIONS - APPLIED TO PROTEINS INVOLVED IN HUMAN DISEASES
… discovery. In this thesis, we show that X-ray holo structures of proteins perform better than NMR holo structures in virtual ligand screening, and we find that among different features, hydrogen bonds in combination with hydrophobic contacts contribute the most to the virtual screening …
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Characterization of Enzymes Involved in Bilin Attachment to Allophycocyanin in the Cyanobacterium Synechococcus sp. PCC 7002
… that both CpcS and HT-CpcU were required to get holo-allophycocyanin with phycocyanobilin attached to the cysteine-81 residue. Absorbance of the product at 615 nm was consistent with holo-monomeric allophycocyanin. Experiments were performed with HT-ApcD ApcB and HT-ApcF ApcA, but size exclusion …
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Vitamin B12 Bioconjugates In Pharmaceutical Design
… What is the uptake of systemically administered holo-intrinisic factor bound to 89Zr-B12 in vivo?</p> <p>Goal: Conduct PET imaging and biodistribution studies in healthy mouse models using IF-89Zr-B12 and assign organ distribution to target receptors.</p> <p>Q3: (Chapter 4) Are functional cubilin …
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Genome - Wide Regulatory Principles of Essential Transcription Factors from Mycobacterium Tuberculosis
… transcription initiation factor holo-WhiB1 in <em>Mtb</em>. Holo-WhiB1 was previously intractable by other approaches, and thus its direct genomic targets were unknown in any species. We first performed a whiB1 knockdown RNA-seq time course in <em>Mtb</em> cells, demonstrating its …
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Investigating intermediates in 6-methylsalicylic acid biosynthesis
… steps, remain cryptic, despite extensive study. Holo 6-MSAS was heterologously expressed in E. coli and purified in two steps. A non-hydrolysable carba(dethia)malonyl-N-acetylcysteamine analogue was synthesised and used to off-load enzyme-bound intermediates from 6-MSAS. In assays with acetyl-CoA …
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Haptic holography : an early computational plastic
This dissertation introduces haptic holography, a combination of computational modeling and multimodal spatial display, as an early computationalplastic In this work, we combine various holographic displays with a force feedback device to image free-standing material surfaces with programmatically …
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ROLE OF LIPOCALIN 2 IN KAINATE-INDUCED NEURODEGENERATION
… inflammation, renal physiology and pathology. Yet, little is known about the functions of LCN2 in the central nervous system (CNS). In view of recent studies that showed an association of LCN2 with apoptosis via LCN2R, the present study investigates the role of LCN2 in kainate …
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Strukturbestimmung von Birkenpollenallergenen und birkenpollenassoziierten Nahrungsmittelallergenen mit NMR-Spektroskopie
… hochaufgelöste dreidimensionale Struktur von holo Bet v 4 zeigt ein kanonisches EF-Hand-Paar in der offenen Konformation. Die hochkonservierte polcalcinspezifische amphipatische COOH-terminale a-Helix deckt lediglich einen Teil der großen hydrophoben Furche auf der Moleküloberfläche von holo …
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Mechanistic Studies and Inhibition of N-hydroxylating Monooxygenases
… The enzyme was purified and characterized in its holo (FAD-bound) and apo (unbound) forms. Pre-steady and steady state kinetics shows that the two forms have different coenzyme preference; apo-AMO prefers NADH while holo-AMO has a higher affinity to NADPH.
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Studies of thiamine biosynthesis
… assembled it was crucial to isolate ThiH in the holo-form. Several expression systems and purification methodologies were investigated. The optimisation of the purification method, together with in vitro chemical reconstitution with exogenous iron and sulfide allowed the successful isolation of …
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Die Wirksamkeit TRAIL-induzierter Nekroptose gegenüber Tumorstammzellen und zellzyklusarretierten Tumorzellen
… Krebszellen, sowie gegenüber Holo-, Mero-, und Paraklonen effektiv ist. Diese Klone weisen einen erhöhten (Holoklone), mittleren (Meroklone), oder geringen Anteil von Zellen mit Stammzelleigenschaften (Paraklone) auf. In dieser Arbeit wurden Holo-, Mero-, und Paraklone von drei …
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Path planning for human planetary surface Exploration in rough terrain
… both planning and navigation purposes, and the Holo-SEXTANT Augmented Reality (AR) navigation solution. The first was used as the primary navigation method for the analog astronaut crew during one of the deployments, while Holo-SEXTANT was used by the analog crew as a technology demonstrator …
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Characterisation of the structural stability of transketolase under biocatalytically relevant conditions
… transitions in the denaturation pathway for holo-TK. The first step, at low urea concentration corresponds to the local restructuring of the thiamine diphosphate (TPP) binding-sites. Next, the dissociation of the TPP cofactors and partial loss of secondary structure produces a form, which is …
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Structural Basis for Protein Recognition, Acyl-substrate Delivery, and Product Release by ACP in the Biosynthesis of Lipid A
… LpxA in complex with one form of ACP (holo-ACP) is presented. The structure reveals three molecules of holo-ACP localize to the C-terminal domain of the LpxA homotrimer, and shows the functional role of this domain is two-fold: ACP recognition and nucleotide binding of UDP-GlcNAc. A …
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Characterization of the Escherichia Coli Acyl Carrier Protein Phosphodiesterase
… for AcpH, resulting in the selective turnover of holo-ACP. I have shown that this is not the case: ethyldethia-ACP is a substrate for AcpH and overexpression of this enzyme results in increased resistance to N-pentylpantothenamide. I have also shown that CoA synthesis is inhibited upon treatment …
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Characterization of Slr1098, a Protein with Similarity to the Bilin Lyase Subunit CpcE from the Cyanobacterium Synechocystis sp. PCC 6803
… interaction between Slr1098 and both apo- and holo-phycocyanin, but not to apo-allophycocyanin. Slr1098 blocked bilin addition at Cys-82 on CpcB by the CpcS/CpcU bilin lyase. Size exclusion chromatography and sucrose density gradient analysis of complexes formed suggest that Slr1098 strongly …
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