Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"HL-60 Cells"”.
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Lysosomal Enzymes in Undifferentiated and Differentiated HL-60 Cells
<p>The human promyelocytic leukemia cell line HL-60 has been studied extensively since the time of it's discovery and has been proven to be a valuable tool for the study of the differentiation process. The capacity of the HL-60 cell line to differentiate to macrophage-like cells, which may be …
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The effects of thymic hormones on the proliferation of human myelogenous leukemia cells
… the effects of TF5 on the proliferation of HL-60 human promyelocytic leukemia cells. Direct viable dye cell counting, the MTT reaction, and the clonogenic potential of HL-60 cells were used to determine cell proliferation. The potency and efficacy of TF5 for the inhibition HL-60 cell …
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Characterization of the biochemical and biophysical mechanisms fundamental to receptor-mediated white blood cell accumulation.
… attach to and roll on the activated endothelial cells lining the blood vessel wall. It has recently been shown in vitro that leukocyte-leukocyte interactions mediated by L-selectin and PSGL-1 enhance accumulation of cells. We have recreated these initial cell recruitment events in vitro using …
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Role of BTG2/TIS21/PC3 in differentiation of myeloid leukemia cells and its regulation under stress conditions
… in leukemia and lymphomas; the tumors are highly proliferative and often blocked at an earlier phase than the terminal stage of differentiation. The interrelation and the functional interplay of these two different proteins are not defined yet. We have shown here that the tumor suppressor …
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Autophagy mechanisms in sputum and peripheral blood cells of patients with severe asthma: a new therapeutic target
… in the sputum granulocytes, peripheral blood cells (PBCs), and peripheral blood eosinophils (PBEs) between patients with severe asthma and those with non-severe asthma and investigated the functional effects of autophagy. Methods: We enrolled 36 patients with severe asthma, 14 with non-severe …
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The role of signal regulatory protein alpha in regulating macrophage subset activity in homeostasis and inflammation
… developed a co-culture system between CD47+ HL-60 and K562 cells, and SIRPα+ Mφ subsets in order to investigate whether the regulatory effect of SIRPα-CD47 ligation extends to negative regulation of TLR-mediated TNFα production, and how this differs between Mφ subsets and under various …
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Characterisation of the effects and mechanism of action of rapamycin and genistein on acute myeloid leukemia using high-throughput techniques
… to study the effects of rapa on MV4-11 and THP-1 cells and an 8-plex iTRAQ based methodology was employed to profile the proteome of the MV4-11 and HL-60 cells treated with GEN. We found that rapa had potent anti-proliferative effect on all the AML cell lines tested. We chose the cell lines with …
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Regulation of tumour necrosis factor receptor expression on neutrophils by arachidonic acid and other long chain fatty acids.
… increase in TNF receptor expression on matured HL-60 cells (neutrophil-like cells), a decrease occurs on HUVEC and non-matured HL-60 cells. These data demonstrate a unique effect of AA on neutrophils. The relationship between AA and the anti-inflammatory (0-3 fatty acids, DHA and …
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Plasma membrane-derived vesicles: their role in the terminal differentiation of monocytes and in inhibiting the phagocytosis of apoptotic cells
… various proteins inherent in their parental cells. PMVs are released when cells undergo activation/apoptosis via blebbing and shedding and have a function in intercellular communication. Exposition of phosphotidylserine (PS) on the outer membrane leaflet that mark them as a biologically …
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Quantitative Image Analysis in the Study of Neutrophil and Neutrophil-Like HL-60 Chemotaxis
Neutrophils are fast-moving first responders of the innate immune system. External chemoattractant signals result in neutrophil polarization: the neutrophil forms a leading edge (front) which constantly protrudes and retracts actin-rich pseudopods, and a contractile myosin-enriched trailing edge …
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Molecular signaling programs underlying neutrophil polarity and chemotaxis
Neutrophils adopt a highly polarized morphology and integrate many matrix-specific signals to undergo specialized migratory events to pursue invading pathogens. While these matrix-specific signaling events have emerged as crucial elements for proper migration and recruitment of neutrophil cells, …
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The effect of P-selectin pattern width on HL-60 cell rolling behavior
… band patterns on the rolling behavior of HL- 60 myeloid cells along edges of the band patterns was studied. The P-selectin and polyethylene glycol (PEG) pattern was produced via microcontact printing using a polydimethylsiloxane (PDMS) stamp. HL-60 cells were flowed across the pattern …
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Digital image analysis of HL-60 proliferation and granulocytic differentiation.
… and granulocytic differentiation of HL-60 leukemia cells which were induced to differentiate by retinoic acid (RA). HL-60 cells were synchronized by density arrest (DA), double thymidine block (TB), or flow cytometric sort (FCM). Following release from the 3 modes of synchrony, …
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Characterization of a new miniaturized hollow-fiber bioreactor for cultivation of cell lines and primary cells to improve cytostatic drug testing in vitro
… membranes. Due to a transparent housing, cells can be observed by microscopy during culture. The leukemic cell lines CCRF-CEM, HL-60 and REH were cultivated up to densities of 3.5 x 107/ml without medium change or manipulation of the cells. Growth and viability of the cells in the …
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Mechanisms of Cytokine-Induced IGF -I Insensitivity in Cancer Cells
… synthesis in human MCF-7 breast adenocarcinoma cells, T-47D breast ductal carcinoma cells, murine FDCP progenitor myeloid cells and human promyeloid HL-60 cells. Flow cytometry confirmed that all three cytokines suppress IGF-I-induced DNA synthesis by preventing cells from entering the S phase …
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Genomics and virulence factors of Fusobacterium necrophorum
… universal within the strain collection by PCR. HL-60 cells subjected to aliquots of concentrated high molecular weight culture supernatant, predicted to contain the secreted leukotoxins of strains JCM 3718, JCM 3724 and ARU 01, were killed in a dose-dependent manner. The cytotoxic effect of the …
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Anaplasma phagocytophilum remodels its host cell-derived vacuole into a protective niche by redecorating the vacuolar membrane with select Rab GTPases and bacterial proteins
… Diverse vacuole-adapted pathogens; including Chlamydia, Legionella, and Salmonella; selectively recruit host Rab GTPases to their vacuolar membranes to establish replicative permissive niches within their host cells. Rab GTPases coordinate many aspects of endocytic and exocytic cargo delivery. …