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Showing 1 to 20 of 46 for “"HL-60"”.
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Lysosomal Enzymes in Undifferentiated and Differentiated HL-60 Cells
<p>The human promyelocytic leukemia cell line HL-60 has been studied extensively since the time of it's discovery and has been proven to be a valuable tool for the study of the differentiation process. The capacity of the HL-60 cell line to differentiate to macrophage-like cells, which may be …
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Digital image analysis of HL-60 proliferation and granulocytic differentiation.
… and granulocytic differentiation of HL-60 leukemia cells which were induced to differentiate by retinoic acid (RA). HL-60 cells were synchronized by density arrest (DA), double thymidine block (TB), or flow cytometric sort (FCM). Following release from the 3 modes of synchrony, …
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Investigation of Neutrophil-Like HL-60 Cell Migration in a 3D Collagen Matrix
… a collagen gel that contained neutrophil-like (dHL-60) cells. Our experimental setup and computational analysis allowed us to study the cell migration indicators such as percentage and velocity of motile cells in a 3D environment. Evaluating these cell migration features showed 10 nM …
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The effect of P-selectin pattern width on HL-60 cell rolling behavior
… band patterns on the rolling behavior of HL- 60 myeloid cells along edges of the band patterns was studied. The P-selectin and polyethylene glycol (PEG) pattern was produced via microcontact printing using a polydimethylsiloxane (PDMS) stamp. HL-60 cells were flowed across the pattern …
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Quantitative Image Analysis in the Study of Neutrophil and Neutrophil-Like HL-60 Chemotaxis
Neutrophils are fast-moving first responders of the innate immune system. External chemoattractant signals result in neutrophil polarization: the neutrophil forms a leading edge (front) which constantly protrudes and retracts actin-rich pseudopods, and a contractile myosin-enriched trailing edge …
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The effects of thymic hormones on the proliferation of human myelogenous leukemia cells
… the effects of TF5 on the proliferation of HL-60 human promyelocytic leukemia cells. Direct viable dye cell counting, the MTT reaction, and the clonogenic potential of HL-60 cells were used to determine cell proliferation. The potency and efficacy of TF5 for the inhibition HL-60 cell …
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Untersuchungen zur Signaltransduktion Ajoen-induzierter Apoptose in Leukämiezellen
… Mechanismus der Apoptoseinduktion durch Ajoen in HL-60 Zellen zu Grunde liegt. Dirsch et al. 5 zeigten bereits 1998, dass Ajoen Apoptose in humanen akut-myeloischen Leukämiezellen (HL-60) induziert. Weiterhin zeigte sich eine dosis- und zeitabhängige Produktion der reaktiven Sauerstoffspezies …
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Untersuchung von Zinksignalen während Aktivierung und Reifung in Monozyten
… Experiments with the myeloid cell line HL-60 showed that short-term zinc signals after stimulation with lipopolysaccharide (LPS) were characteristic for differentiated, tumor necrosis factor-alpha secreting cells, and were essential for LPS-induced signal transduction. LPS-induced zinc …
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The role of signal regulatory protein alpha in regulating macrophage subset activity in homeostasis and inflammation
… developed a co-culture system between CD47+ HL-60 and K562 cells, and SIRPα+ Mφ subsets in order to investigate whether the regulatory effect of SIRPα-CD47 ligation extends to negative regulation of TLR-mediated TNFα production, and how this differs between Mφ subsets and under various …
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Protektive in vitro Wirkung mitochondrialer Entkoppler auf die Apoptose in Leukämiezellinien
… des lymphatischen (CCRF-CEM) und myeloischen (HL-60) Ursprungs zugrunde liegen. Der Effekt mitochondrialer Entkoppler auf die durch Che und Sts induzierte Apoptose sollte ausserdem mit der Wirkung bekannter Modulatoren des programmierten Zelltodes wie Caspasehemmer zVAD und …
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Characterisation of the effects and mechanism of action of rapamycin and genistein on acute myeloid leukemia using high-throughput techniques
… to profile the proteome of the MV4-11 and HL-60 cells treated with GEN. We found that rapa had potent anti-proliferative effect on all the AML cell lines tested. We chose the cell lines with the lowest and highest IC50, MV4-11 and THP-1 respectively, for functional characterisation. …
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Untersuchungen zur funktionellen Bedeutung von Gangliosiden bei der E-Selektin-vermittelten Zell-Zell-Adhäsion
… die Granulocyten aktiviert und adhärieren schließlich im dritten Schritt fest an die Endothelzellen. Der vierte und letzte Schritt beinhaltet die Durchdringung der Endothelzellbarriere (Diapedese) und das anschließende Einwandern in das darunterliegende Gewebe. Cytokin-induzierte …
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Plasma membrane-derived vesicles: their role in the terminal differentiation of monocytes and in inhibiting the phagocytosis of apoptotic cells
… the role of PMVs, in the differentiation of HL-60 promonocytes. The myeloid differentiating agents ATRA, PMA and histamine, which inhibit promonocyte proliferation, induced an intracellular Ca2 +-mediated PMV (as opposed to exosome) release from HL-60 promonocytes. These PMVs caused HL-60 …
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Bystander-Effekt des Peroxynitrit und der toxische Effekt von Cu(II)-SOD
… <br>Apoptoseinduktion durch Promyelozyten (HL 60) in transformierten Fibroblasten hängt von der Anzahl der Effektor- und Zielzellen ab. Die Apoptoseinduktion durch HL 60 wurde mit Granulozyten als Effektorzellen verglichen.
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Characterization of the biochemical and biophysical mechanisms fundamental to receptor-mediated white blood cell accumulation.
… cell aggregation, or cell accumulation. HL-60 cells or neutrophils in the presence of platelet microparticles were withdrawn over HL-60 cells prebound to a glass slide. We have shown that platelet microparticles can mediate cell aggregation, cell-cell interactions, and enhanced cell …
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Avaliação do potencial antileucêmico de adutos aromáticos de morita-baylis-hillman.
… using the culture of some leukemic cells, HL - 60, K562, K562 - Lucena and MOLT - 4, as well as normal cells from peripheral blood mononuclear cell (PBMC). The effect of these MBHA synthetic molecules were evaluated by MTT reduction and neutral red uptake techniques and the mechanism of …
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Etablierung von Methoden zur Bioevaluation antitumoraler Naturstoffe, Metabolite und Analoga
… erwiesen sich hierbei als Methoden der Wahl. Illudin S 11a konnte jedoch nicht isoliert werden. Daneben konnte eine Reihe von Verfahren zur Evaluierung von Testsubstanzen etabliert werden. Die Untersuchungen der Substanzeigenschaften (siehe 2.5 bis 2.8) lieferten nützliche und verlässliche …
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