Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"HL-1"”.
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Nesfatin-1 Regulation of Cardiovascular Functions in Zebrafish and HL-1 Cardiomyocytes
… was detected in the cytoplasm of mouse HL-1 cardiomyocytes. High glucose increased NUCB2 mRNA expression in HL-1 cells. Genes involved in apoptosis, including Akt1, Caspases 1, 2, 3, and TNF were upregulated in the presence of 10 nM nesfatin-1. We also observed that NUCB2 mRNA expression …
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Collagen-Glycosaminoglycan Scaffold Systems to Assess HL-1 Cardiomyocyte Beating and Alignment
… examine the alignment and beating potential of HL-1 cardiomyoctyes in a 3D environment. Collagen-glycosaminoglycan scaffolds have been used extensively to probe the behavior of mature cells in vitro, but have not yet been designed for cardiac applications. In order to recapitulate key properties …
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Calcium Influx via the T-Type Calcium Channel Plays a Permissive Role in Proliferation of Mouse Embryonic Hl-1 Cells
… cell types. An atrial myocyte cell line (HL-1) that selectively expresses T-type calcium current was employed to show that inhibiting calcium influx through the T-type calcium channel inhibits cellular proliferation. Drug dosage studies demonstrate that the T-type calcium channel …
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Sphingosine-1-Phosphate and Fingolimod (FTY720) Regulate ICl,swell In HL-1 Cardiac Myocytes via Intracellular Binding And Mitochondrial ROS Production
<p>Swelling-activated Cl− current (ICl,swell) is an outwardly-rectifying current that plays an important role in cardiac electrical activity, cellular volume regulation, apoptosis, and acts as a potential effector of mechanoelectrical feedback. Persistent activation of ICl,swell has been observed …
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Silicium Nanodrähte für die extrazelluläre Ableitung elektrischer Aktivität
… nanowires are promising candidates for highly sensitive biosensors. We designed and fabricated nanowire transistors suitable for the extracellular detection of electrical activity. These sensors were characterized thoroughly and tested with HL-1 and HEK cells. The tests with HL-1 cells …
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Organization of energy transfer and feedback regulation in oxidative muscle cells
… hypercontraction in normal cardiomyocytes and in HL-1 cells, 2) to confirm existence of ICEUs by mathematical modeling of real experimental data, 3) to investigate whether ICEUs exist also in human heart atrium and in musculus gluteus medius (MGM), 4) to investigate whether these units may in MGM …
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Myocardial ischemia and reperfusion injury, clinical and experimental studies
… chain reaction. Mouse cardiomyocytes (HL-1 cells) were analysed after exposition to hypoxia. The HL-1 cells were further investigated with aspects of FKBP12 and FKBP12.6 release in hypoxia, energy depletion, acidosis, ROS activation and re-establishing of physiologic conditions, …
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ROLE OF ENDOTHELIN-1 IN THE REGULATION OF THE SWELLING-ACTIVATED Cl- CURRENT IN ATRIAL MYOCYTES
… Cl- current that was fully blocked by the highly selective ICl,swell inhibitor DCPIB and by osmotic shrinkage. Selective ETA blockade (BQ123), but not ETB blockade (BQ788), fully suppressed the ET-1-induced current. ET-1-induced ICl,swell was abolished by blockade of EGFR kinase (AG1478) and …
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The process of autophagy in an in vitro model of myocardial ischemia-reperfusion injury
… of ischemia (hypoxia) involving exposure of HL-1 murine atrial cardiomyocytes to two concentrations of oxygen (0.5% and 2.0%) and 2-deoxyglucose. </p> <p>Hypoxia, irrespective of the oxygen concentration, caused a dramatic drop in cellular ATP and an increase in the phosphorylation of AMPK …
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Communicating with electrogenic cells
… coupling was demonstrated by recording from HL-1 cells. The ability to interface single cells was shown by recording from HEK cells that were synchronously patch-clamped. In addition, the generation of dened neural networks was tackled, and patterned, low density neural networks were …
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Pathophysiological changes in cardiomyocyte metabolism and bioenergetics during stress-induced cardiomyopathy.
… For the study was used immortalized cell line of HL-1 cardiomyocytes. Cardiomyocytes were expose to extreme dose of isoprenaline and chronic hypoxia. Real-time cell metabolic analysis, total lipid analysis, high-performance liquid chromatography (HPLC) and mass spectrometry for lipidomic spectrum …
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Investigation into the mechanism of Nox2 NADPH oxidase-dependent signalling in doxorubicin cardiotoxicity
… differentially expressed genes (P<0.1) and highlighted ‘Cell Death and Survival’ as the network of most significance to the dataset. Further examination using heat maps highlighted cell death and survival functions, with a focus on 5 genes associated with cardiomyocyte apoptosis. Of these …
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Calcium Channel Gamma(6) Subunit: In Search of Its Functions and Mechanisms of Action
… that gamma 6 also inhibits LVA currents in HL-1 cardiomyocytes, confirming its function in native cells. Previous studies from our lab indicated that the first transmembrane domain of gamma6 is responsible for its inhibitory function. Using mutational analysis, we further identified the …
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Chemical Biology Tools for Biochemical and Functional Analysis of Myosin Phosphatase
… tight temporal control of PPP1R12C levels in HL-1 atrial cardiomyocytes. Functional assays demonstrated that stabilization of PPP1R12C reduced MLC2a phosphorylation and contractile amplitude in atrial cells, mimicking the hypocontractile phenotype observed in AF. Conversely, PPP1R12C depletion …
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Pressure drop, liquid holdup and mass transfer in a graphite fibre bed with upward co-current gas-liquid flow
… The correlation for the total liquid holdup is hL = 1 - 0.907 L 0.362 G 0.301 where hL = liquid holdup. The overall mass transfer capacity was determined by the electrochemical reaction method with the electro-reduction of oxygen to peroxide. The electrochemical reactor used consisted of …
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Mitochondrial Dynamics: Exploring A Novel Target Against Myocardial Ischemia-Reperfusion Injury
… model of hypoxia-reoxygenation (HR) in cultured HL-1 cardiomyocytes, we tested three hypotheses:</p> <p>I. subcellular redistribution of DRP1 is i) triggered by HR, and ii) plays a mechanistic role in HR-induced cytochrome c release and cell apoptosis; </p> <p>II. inhibition of DRP1 translocation …
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Extracellular stimulation of individual electrogenic cells with micro-scaled electrodes
… recordings of electrical activity from the HL-1 cardiac myocyte-like cell line were performed. The developed MEAs and SIROF layers showed excellent electrochemical properties, stability, and biocompatibility, and were successfully applied for extracellular stimulation and recording. Hence, …
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The interplay between pro-death and pro-survival signaling pathways converging at mitochondria in myocardial Ischemia/Reperfusion : Apoptosis meets Autophagy
… the single cell. Simulated I/R (sI/R) of cardiac HL-1 cells was employed as an in vitro model of I/R injury to the heart. To assess macroautophagy autophagosome generation and degradation (autophagic flux) was quantified, as determined by steady-state levels of autophagosomes in relation to …
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EXPLORING THE ANTI-INFLAMMATORY POTENTIAL OF ANTHOCYANIN-RICH CROPS
… sono state in grado di proteggere cardiomiociti HL-1 trattati con DOX con la stessa efficacia ottenuta con una dose 12.5 volte maggiore di ACNs libere. In un modello in vitro di assorbimento intestinale basato su cellule Caco-2, però, la principale ACN del mais rosso, cianidina 3-glucoside …
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GPCR to Channel Communication in a Membrane Signaling Pathway
… and electrophysiological properties of the HL-1 cell membrane are replicated in a reaction-diffusion simulation. Based on the simulation, I propose that both protein clustering and the positive bias are crucial to the M2R-GIRK channel signaling pathway.</p>