Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"HITS-CLIP"”.
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Genome-Wide Decoding of mRNP and miRNA Maps
… isolated by crosslinking immunoprecipitation (HITS-CLIP). HITS-CLIP analysis of the neuron-specific splicing factor Nova provides genome-wide maps of Nova-RNA interactions in vivo and leads to a new finding that Nova may regulate the processesing of some miRNAs. Furthermore, HITS-CLIP analysis …
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RNA Dynamics in T Cell Activation
… isolated by cross-linking immunoprecipitation (HITS-CLIP). Here we used HITS-CLIP to define genome-wide maps of both Ago and HuR RNA binding sites in freshly isolated primary human T cells in the resting state and after one hour of stimulation with anti-CD3 and anti-CD28 antibodies. We also …
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Investigating the Role of Muscleblind-Like 1 in the Suppression of Breast Cancer Progression
… in breast cancer cells was carried out using HITS-CLIP. Transcriptome-wide analysis of MBNL1-dependent transcript stability and MBNL1 HITS-CLIP data revealed that globally, transcripts directly bound by MBNL1 are stabilized by MBNL1. Two transcripts, DBNL and TACC1, were identified as …
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RNA Deregulation in Metastatic Breast Cancer
… cross-linked immunoprecipitation (HITS-CLIP) provides both the identity of regulated transcripts and the location of RBP regulation within a transcript. For the post-transcriptional regulatory protein Argonaute (AGO), which binds mRNA in complex with small non-coding microRNAs …
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Transcriptome-Wide Characterization of APOBEC1-Catalyzed RNA Editing Events in Macrophages
… isolated by cross-linking immunoprecipitation (HITS-CLIP) of the Argonaute (Ago) proteins was performed on wild-type and APOBEC1-deficient cells. HITS-CLIP yielded transcriptome-wide maps of Ago binding and potential miRNA seed regions. While there was considerable overlap between loci targeted …
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RNA Localization and Translational Regulation on the Endoplasmic Reticulum
… encoding endomembrane transmembrane proteins. HITS-CLIP and PAR-CLIP analyses of the AEG-1 mRNA interactome of human hepatocellular carcinoma cells revealed a high enrichment for mRNAs encoding endomembrane organelle proteins, most notably encoding transmembrane proteins. AEG-1 binding sites …
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A Stress-Induced TRNA Depletion Response Mediates Codon-Based Translational Repression and Growth Suppression
… isolated by crosslinking immunoprecipitation (HITS-CLIP) of hnRNPA1 and SSB confirmed the mass spectrometry results and identified endogenous reciprocal interactions between the protein and tRF. Binding of this tRF to hnRNPA1 inhibits destabilization of endogenous targets of this RNA binding …
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A Network Approach to Understanding miRNA Regulation in Adipose Tissue
… binding activity. Most notably, we used Ago HITS-CLIP to comprehensively map mRNA:miRNA interactions in brown and white fat, revealing 21,281 unique miRNA binding sites in 6,717 genes. Most binding sites were shared between brown and white fat, although reads per binding site and reads per …
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Characterization of the Interactions Between Kaposi’s Sarcoma-Associated Herpesvirus ORF57 and Its RNA Partners
… by crosslinking and immunoprecipitation (HITS-CLIP) protocol to identify novel host and viral targets of ORF57 in the context of viral infection. Bioinformatic analysis of potential host ORF57 targets reveals that ORF57 binding is enriched near the 5' end of the transcripts and often close …
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Identification and Characterization of Novel Mechanisms of Functional and Structural Synapse Remodeling: Focus on Vav Guanine Nucleotide Exchange Factors and MEF2 Transcription Factors
… isolated by cross-linking immunoprecipitation (HITS-CLIP) of FMRP, I find a large overlap of MEF2-induced transcripts and FMRP-associated mRNAs, consistent with their shared roles in synapse elimination. More specifically, protocadherin 17 (Pcdh17) mRNA is induced by MEF2 and exhibits …
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A Genomic Portrait of Hepatitis C Virus and MicroRNA-122
… sequencing and crosslinking immunoprecipitation (HITS-CLIP) experiments of human Argonaute (AGO) during HCV infection. We demonstrate robust AGO binding on the 5' untranslated region of HCV RNA at known and predicted miR-122 sites, thereby establishing conclusive biochemical evidence of endogenous …