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Showing 1 to 20 of 22 for “"HILIC"”.
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An exploration of the mechanism of hydrophilic interaction chromatography (HILIC) and the application of HILIC in metabolomic profiling
The aim of this study was to investigate hydrophilic interaction chromatography (HILIC) and its application to metabolic profiling. The effect of different mobile phase modifiers on the retention behaviour of acidic and neutral test probes was studied on silica gel based columns which confirmed the …
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New thin shell silica particles and HILIC phases for liquid chromatography: synthesis, characterisation and functionalisation
… quaternisation with sodium chloroacetate for HILIC separation of polar analytes. All bonded phase materials were characterised by microanalysis, thermogravimetric analysis (TGA) and diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy. The bonded thin shell and core shell …
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A study of the mechanism of hydrophilic interaction chromatography and its application in the analysis of medical drugs and metabolites
Hydrophilic interaction chromatography (HILIC) has been the subject of a few excellent reviews in recent years and most focused on different factors which impact on the separation of compounds in HILIC mode including column temperature, mobile phase composition, pH, buffer type and concentration. …
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An Evaluation Of UHPLC-MS Technology Encompassing Elevated Temperatures, Low Viscosity Operation and Recently Developed Stationary Phase Materials
… and neutral test substances. An evaluation of HILIC column efficiency was undertaken by assessing the performance merits of several commercially available packings using the Knox/van Deemter and kinetic plots approaches. This work centred on evaluation using realistic test substances on …
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Synthesis & retention mechanisms of novel mixed mode stationary phase for detection of specific polar low molecular weight biological samples
… of novel mixed mode stationary phases for Hydrophilic Interaction Liquid Chromatography (HILIC). HILIC is a rapidly emerging chromatographic mode that is finding great applicability in the analysis of polar organic molecules. In addition, there is a chapter on the analysis of Bisphenol A and …
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In-column polymer modifications: Advancing polymer bonded phases
… polymer chains (acrylamide) give excellent hydrophilic liquid chromatography (HILIC), a common method used in glycosylation analysis. These columns are limited by lack in reproducibility and stability. A new method to make polymer bonded phases to give more reproducible, stable, and higher …
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Vícerozměrné separace v kapalné fázi
… separačních platforem. Realizována byla HILIC-MALDI-MS analýza glykanů, dvourozměrná separace peptidů z tryptického digestu BSA využívající off-line spojení kapilární izoelektrické fokusace s vyvinutým gradientovým miniaturizovaným LC systémem a platforma umožňující realizaci on-line …
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Studies of UHPLC-MS performance and application to rapid sensitive and robust drug analysis
… affect the chromatographic performance of hydrophilic, basic analytes. Manipulation of mobile phase pH suppresses the protonation of basic analytes for improved retention, peak shape and resolution. This is exemplified by the ephedrines, which are inherently difficult to separate by …
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Identification and enantioselective determination of protein amino acids in fertilizers by hydrophilic interaction and chiral chromatography coupled to tandem mass spectrometry /
… assay in HPF samples was developed in hydrophilic interaction chromatography (HILIC) mode with tandem MS/MS detection. The developed method delivers fast analysis and superior specificity. Enantioselective HPLC on a glycopeptide antibiotic teicoplanin-based chiral stationary phase coupled to …
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Structural Characterization of Donkey Lactoferrin: Amino Acid Sequence and Glycan Compositions
… profile by chymotrypsin digestion, TiO2 and HILIC enrichment strategy, reversed-phase high performance liquid chromatography, electrospray mass spectrometry, high collision dissociation fragmentation, is reported. 26 different glycan compositions were identified, linked to the protein …
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Identification of the metabolites responsible for colour stability from different lamb sires and elucidation of the underlying biochemical pathways : A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy in Biochemistry at Lincoln University
… In metabolomics, using a normal phase hydrophilic interaction chromatography-mass spectrometry (HILIC-MS) approach, taurine, glutamic acid, aspartic acid and cytidine were identified. As a complementary measure to HILIC by using the reverse-phase C18 column in lipidomics, the approach has …
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Development of liquid chromatography mass spectrometry methods for the monitoring of ropivacaine, endogenous steroids and dihydroartemisinin
… in human plasma using equilibrium dialysis and HILIC chromatography coupled with a high resolution mass spectrometery. In this chapter a highly sensitive method for the determination of the free and bound portion of ropivacaine in patients undergoing knee and hip joint surgery was developed. …
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Applications of LC-MS metabolomic profiling in inflammatory bowel disease and the development of derivatisation methods to enhance selective detection of certain compound classes
… profiled by carrying out chromatography on a ZICpHILIC column in combination with high resolution mass spectrometry.;It was possible to separate the different classes of urine samples on the basis of their metabolomic profiles by modelling with data using orthogonal partial least squares analysis …
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Optimising the Wider Energy Metabolome of Microbes Using Targeted UHPLC-MS/MS Present in Bio-Electrochemical Systems for Carbon Dioxide Conversion to Platform Chemicals
… a single analytical run using Zwitterionic Hydrophilic Interaction Chromatography (Z-HILIC) coupled to tandem mass spectrometry. The resulting workflow offers high sensitivity, strong reproducibility, and low limits of detection and quantification, making it suitable for the analysis of …
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Discovery of Quinic Acid Derivatives as Oral Anti-inflammatory Agents
… KZ-41, we developed and validated a novel hydrophilic interaction liquid chromatography–tandem mass spectrometry (HILIC-MS/MS) analytical assay. An analog of KZ-41 was used as internal standard (IS). KZ-41 and the IS were obtained by protein precipitation and separated by HILIC chromatography …
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Characterization of Gangliosides and Fatty Acids in Extracellular Vesicles from Human Milk
… ganglioside content was determined by GC-MS and HILIC-HPLC/MS-MS. The relative content of fatty acids and gangliosides within human milk EVs was assessed in relation to the relative content in the whole human milk. The EV isolation procedure yielded 2.08 x 10<sup>10 </sup>EV particles/mL of human …
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Gut Health Benefits of Natural and Alkali-Processed Cocoa (Theobroma cacao) with and without Inulin
… (total flavanols) assays, thiolysis , and HILIC UPLC-MS/MS. Treatments of cocoa and inulin were made in 1:2 cocoa:inulin and 1:4 cocoa:inulin mixtures for both natural and alkalized cocoas. Cocoa mixtures, in addition to both cocoa powders and inulin alone, were subjected to an in-vitro …
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Evaluation of cocoa (Theobroma cacao) bean processing strategies to enhance alpha-glucosidase inhibitory activity of dietary cocoa
… to quantify Maillard reaction products (MRP). A HILIC-UPLC MS/MS method was developed to more efficiently and sensitively quantify cocoa flavanols with high degrees of polymerization (DP) produced during processing. Overall, cocoa processing significantly (p<0.05) decreased the total phenolic and …
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Synthesis and application of tagging agents in mass spectrometry-based targeted metabolomics studies
… sugars. The detection of the sugars by hydrophilic interaction chromatography (HILIC) in combination with mass spectrormetry (MS) was greatly improved along with chromatographic resolution allowing for easy identification of sugar isomers. In Chapter three, analysis of short chain fatty acids …
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Applications of LC-MS/MS in forensic toxicology for the analysis of drugs and their metabolites
… method for these metabolites using a novel hydrophilic interaction liquid chromatography column was validated and applied to routine forensic casework. Ninety urine case samples were divided into three groups depending on the ethanol concentration found in blood and analysed by the developed …
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