Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"HIF1A"”.
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Identification of Novel HIF1A Target Genes That Regulate Tumor Progression and Metastasis
… from late stage carcinomas originating in PyMT+; Hif1a floxed mice. These MTECs were exposed to either Adenovirus-beta-gal or -Cre to create wild-type (WT) and knockout (KO) cells, respectively. Deletion of HIF-1 activity reduced primary tumor growth by ~60% and the formation of lung …
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The p97 cofactors UBXN7 and UBXN8 interact with and modulate the function of cullin-RING complexes and Fanconi anaemia proteins FANCD2/FANCI, respectively
… and causes the accumulation of non-ubiquitylated HIF1a (CRL2 substrate). Both effects are strictly UIM-dependent and occur only when UBXN7 contains an intact UIM. We also show that HIF1a carrying long ubiquitin-chains can interact with an alternative ubiquitin-binding protein, which is independent …
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Differential Angiogenic Capability and Hypoxia Responses in Glioma Stem Cells
… is not a general stem cell response. Targeting HIF1a or HIF2a in GSCs using short hairpin RNA (shRNA) inhibited neurosphere formation efficiency, indicating a requirement for HIFs in cancer stem cell self-renewal. HIF1a and HIF2a were also necessary for VEGF expression in GSCs, but HIF2a was not …
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Hypoxic Adaptation and Arsenic Trioxide Treatment in Small Cell Lung Carcinoma
… genes. We further show that knockdown of HIF1A using siRNA or shRNA, is not significantly affecting the cell viability of cultured SCLC cells at moderate and severe hypoxia or tumor take and tumor growth in SCLC xenografts. We found that SCLC cells are dependent on glutamine metabolism for …
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Dissecting adult epithelial cell plasticity using a model of oesophageal-to-skin lineage conversion
… targeting the hypoxia-inducible factor-1α (HIF1a) and its downstream target SRY-box transcription factor 9 (SOX9). These results unveiled that the HIF1a-SOX9 axis poses a barrier to oesophageal-to-skin lineage conversion. In turn, when this barrier is lifted cells respond better to the …
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The Hypoxic Adenosine Response Modulates Macrophage Differentiation and Contributes to Lung Disease
… silencing of hypoxia inducible factor 1a (HIF1A) as a means to attenuate pro-fibrotic mediator production and pulmonary fibrosis in bone marrow derived macrophages (BMDMs) and in vivo models of bleomycin-induced pulmonary fibrosis. These players will be valuable as potential clinical …
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A Cancer-Specific Study On The Differentially Expressed Protein-Protein Interactions of Fumarate Hydratase
… less understood compared to its other isoforms (HIF1a and HIF2a), this research contributes to bridging that knowledge gap. Our findings suggest that the HIF3a gene is a significant, differentially expressed gene in FH deficient patients.</p>
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Early onset of age related hearing loss in LDHB knock out mouse
… cell, the decrease of NAD+ and increase of HIF1a which probably cause mitochondrial dysfunction. LDHB knock down sensitized hair cell to antibiotics which support important clinical information.
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UNDERSTANDING THE CELLULAR AND GENE-REGULATORY MECHANISMS UNDERLYING THE MESENCHYMAL TRANSITION OF EPENDYMOMA TUMOR CELLS USING OMICS APPROACHES
… factors like NFkB, AP-1, MAF/BACH, MYC, and HIF1A. We are also the first to use single-cell omics data to characterize the ependymal tumor microenvironment and identify pro-inflammatory microglia as an important mediator of the mesenchymal transition of ependymoma tumor cells. Building upon …
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Molecular and Metabolic Regulation of Gastrointestinal Cancer Metastasis
… retention of hypoxia inducible factor 1a (HIF1a) via phosphorylation of serine 641 and serine 643 residues. The final part of this study demonstrates therapeutic targeting of PCK1 and NPTX1-AMIGO2 pathways inhibits cancer metastasis and progression in multiple <em>in vivo</em> models. In …
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Oncostatin M Receptor Overexpression Promotes Tumour Progression in Squamous Cell Carcinoma, via Hypoxia Signalling and Multiple Effects on the Tumour Microenvironment
… to induce Hypoxia inducible factor 1a and 2a (HIF1a, HIF2a) signalling in normoxic conditions, to promote pro-angiogenic signalling. Furthermore, hypoxic conditions were found to enhance OSM signalling in OSMR overexpressing cells leading to increased expression of markers of epithelial to …
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Establishment and characterisation of chemoresistant osteosarcoma cell lines by single and multi-agent induced strategies
… to epithelial gene signalling occurred.SPHK1 and HIF1A were upregulated in most of the resistant models from the PCR array analysis and SPHK1 protein level was also determined to increase in MG-63/CISR8 (2.03 ± 0.08-fold, p=0.034), MG-63/DOXR8 (1.77 ± 0.24-fold, p=0.02), and HOS-143B/CISR8 (3.55 ± …
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INVESTING THE ROLES OF E26 TRANSFORMATION-SPECIFIC HOMOLOGOUS FACTOR IN CYSTIC FIBROSIS LUNG DISEASE
… forskolin-induced swelling while reducing the HIF1a-induced response to hypoxia, thereby modifying the pH of EHF-/- epithelia, compared to EHF+/+ clones. Taken together, our data outlined several mechanisms by which EHF could be linked to CFLD. In conclusion, we hypothesise that any …
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The effects of metformin on the gastrointestinal tract
… may potentially be mediated by AMPK and HIF1A signalling pathways. By inhibiting mitochondrial respiration, metformin also limited the metabolic plasticity of intestinal cells to utilise other metabolic fuels, which instead relied almost exclusively on glycolysis. In summary, this study …
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Studies into the receptor-mediated induction of hypoxia inducible factor 1α.
… has identified tissue-specific expression of HIF1a isoforms that have diminished or absent transcriptional activation capabilities, suggesting that the overall transcription of target genes by HIFla may be modulated by interactions between these isoforms. This thesis focuses on the role of …
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THE EVOLUTION OF THE MODERN CONDITION THROUGH THE LENS OF NEURODEVELOPMENT DIVERSITY: INSIGHTS FROM A PARADIGMATIC REWIRING OF GENE REGULATION
… to the CADPS2 gene and predicted to alter HIF1A binding. These findings implicate a modulation of the rhombic lip maturation tempo, in contrast with previous claims of heterochronous neuronal maturation being a cortex-specific feature. In sum, this thesis establishes a framework going from …
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pH Regulatory Molecules In The Tumour Microenvironment: Modulators Of Aggressiveness And Immune Profile Of Human Hepatocellular Carcinoma
… the hypoxia-inducible factor 1α-related gene (<i>HIF1A</i>) and of the stem cell-associated markers CD24, CD133, EpCAM and KRT19. Moreover, mRNA for <i>CA9</i> and for the different <i>CA12</i> isoforms were associated with tumour grading, thus indicating their possible role in tumour malignancy. …
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Exploring the role and the function of Aryl Hydrocarbon Receptor (AhR) and Aryl Hydrocarbon Nuclear Translocator (ARNT) in T cells
The Aryl Hydrocarbon Receptor (AhR) and the Aryl Hydrocarbon Nuclear Translocator (ARNT) play a role in mediating transcriptional responses to environmental pollutants, including the highly toxic compound 2,3,7,8-tetrachlorodibenzo -p-dioxin (TCDD) but also endogenous physiological ligands. More …
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The role of ABHD11 in the regulation of the hypoxia inducible transcription factors
Hypoxia inducible transcription factors (HIFs) mediate a highly conserved cellular oxygen sensing system, driving a diverse set of transcriptional changes that allow adaptation to hypoxic conditions. However, HIFs can also be activated independently of oxygen by changes in levels of mitochondrial …
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