Global ETD Search
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Showing 1 to 17 of 17 for “"HIF-1a"”.
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유두상갑상선미세암에서혈관내피성장인자(VEGF),혈관내피성장인자수용체-1(VEGFR-1),저산소증유발인자(HIF-1a)의mRNA발현
… 발현을 유도하는 저산소증 유발인자(Hypoxic induced factor 1α, HIF-1α), 각각의mRNA발현정도를 분석하여 신생혈관생성의 발현형의 변화여부를 확인하고 여러 임상 및 병리적 특징과 VEGF mRNA의 발현정도와의 상관관계를 분석 하기 위하여 시행되었다. 대상 및 방법: 유두상 갑성선암 진단 하에 갑상선절제술을 시행한 환자 중 종양의 크기가 10mm이하인14명의 환자를 대상으로 수술 중에 암종양조직과 동측의 정상갑상선조직을 채취하여 mRNA의 손상을 막기 위해 조직을 RNA later용액을 첨가하여 …
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Molecular mechanisms of nutrient mediated regulation of hypoxia inducible factor -1a(HIF-1a) in endothelial cells.
… expression of hypoxia-inducible factor-alpha (HIF-1alpha). In an animal model of diabetes, HIF-1alpha protein expression was seen to increase in the vasculature surrounding the sciatic nerve at ten- and twenty-four weeks of diabetes duration, which was seen to be reversed at twenty-four weeks …
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The regulation of hypoxia-responsive gene expression by hydroxyl radicals and intracellular calcium
… Wegfangen der OH· durch DHR die Aktivität der HIF-Prolyl-Hydroxylase (PHD) und die Interaktion des von Hippel-Lindau Proteins (VHL), was somit zu einer vermehrten HIF-1a Proteinexpression, Kerntranslokation, Transaktivierung und Steigerung der Expression der HIF-1a Zielgene führt. Verschiedene …
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Μελέτη της ακτινικής δερματίτιδας σε ασθενείς με καρκίνο μαστού : συσχέτιση με κλινικές και παθολογοανατομικές παραμέτρους
… ανίχνευση της έκφρασης των πρωτεϊνών Κi67, ATM, HIF-1a, iNOS και aGluc. Τα ευρήματα συσχετίστηκαν με την εκδήλωση της ακτινικής δερματίτιδας, λαμβάνοντας υπόψη το σωματικό βάρος και τον τύπο δέρματος (ταξινόμηση Fitzpatrick). Τα τελικά δεδομένα αξιολογήθηκαν περαιτέρω μέσω διαφόρων μονοπατιών και …
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A novel role for Sprouty2 in co-ordinating airway and vascular growth of the fetal lung.
… transcription factor, Hypoxia Inducible Factor 1a (HIF-1a). The FGF receptor antagonist, Sprouty2 (SPRY2), influences the duration of this signalling activity and thus governs periodical airway and vascular branching. However, the mechanism behind this coordination is unknown. The aim of this …
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Hypoxia-induced phenotypic modulation of human neuroblastoma cells
… to hypoxia are the hypoxia-inducible factors HIF-1a and HIF-2a. Intriguingly, HIF-2a, but not HIF-1a, was detected adjacent to blood vessels in neuroblastoma specimen, indicating a role for HIF-2a at more physiological oxygen levels. Analysis of HIF-protein levels in neuroblastoma cells …
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Characterising mechanisms of regulation of hypoxia-inducible factors.
… by the Hypoxia-inducible factor protein, HIF. HIF is a heterodimer consisting of one of three alpha subunits (HIF-1a, HIF-2a or HIF-3a) and a beta subunit called Amt. The activation of HIF by hypoxia is a multistep process involving increases in both protein stabilisation and …
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Exploring the role and the function of Aryl Hydrocarbon Receptor (AhR) and Aryl Hydrocarbon Nuclear Translocator (ARNT) in T cells
… Cre recombinase ‘knocked in’ to the CYP1A locus (CYP1A1Cre+/-) was backcrossed to the R26REYFP reporter mouse. In R26REYFP mice, a gene encoding EYFP is knocked into the ubiquitously expressed Rosa26 locus preceded by a loxP flanked stop sequence. CYP1A1 expression is controlled by …
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Der Transkriptionsfaktor JunB in der zellulären Hypoxie-Antwort und in der Angiogenese : Entschlüsselung der übergeordneten Signalwege und Identifizierung und funktionelle Analyse des JunB-Zielgens CBFbeta
… Bedingungen über den Transkriptionsfaktor HIF induziert wird. JunB-defiziente Mäuse zeigen eine vaskulären Phänotyp und sterben zwischen Tag 8,5 und 10,5 der Embryonalentwicklung. Die auffallenden phänotypischen Übereinstimmungen zwischen JunB-defizienten Embryonen und Mäusen ohne …
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Characterisation of the Effect and Functional Significance of Fcγ Receptor Crosslinking on Metabolic Processes in Macrophages
… rapamycin (mTOR) and hypoxia-inducible factor (HIF)1α. Moreover, this glycolytic switch was required to generate a number of pro-inflammatory mediators and cytokines. Inhibition of glycolysis, or genetic depletion of HIF1α in macrophages resulted in the attenuation of IL1β and other inflammatory …
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The Effects of Metronomic and Maximum-Tolerated Dose Chemotherapy in Colorectal Cancer Angiogenesis: A Combined Approach Using Endoscopic Diffuse Reflectance Spectroscopy and mRNA Expression
… tumors treated with MET presented higher Hif-1a, Aldoa and Pgk1 expression, indicating increased glycolytic activity. Taken together, this work highlights that the opportunities and challenges of MET to treat CRC can be better understood by using combined modalities that complement the …
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The role of the chromatin remodeller ISWI in the cellular response to hypoxia
The Hypoxia Inducible Factor (HIF) is a master regulator of the cellular response to hypoxia. Its levels and activity are controlled by dioxygenases called Prolyl-Hydroxylases and Factor Inhibiting HIF (FIH). In order to activate genes, HIF has to access sequences in DNA that are integrated in …
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Investigation of hypoxia and hyperglycaemia mediated DNA damage in human endothelial cells.
… damage. Further investigation reveals a role for HIF-1a in the development of endothelial DNA damage which is hypoxia-dependent. Attempts to counteract the observed cytotoxicity to endothelial cells using the flavonoid antioxidant, silymarin, revealed that silymarin is a potent cytoprotective …
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Functional Heterogeneity and Metabolic Adaptation of Fibroblasts In Cancer
… by hypoxia-induced epigenetic modification of HIF-1a and rate-limiting glycolytic enzymes. As an extension to this work, we further explored fibroblasts’ contributions to cancer initiation. Here, we leveraged a previously reported mouse model in which Tgfb signaling in fibroblasts has shown to …
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MT1-MMP enhances cell survival and proliferation via roles unrelated to metabolic transcript levels in MCF7 breast cancer cells
Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a multifunctional protease implicated in cancer aggressiveness for its ability to promote proliferation, migration, and invasion, particularly at low expression levels. Due to its multidomain structure and cell signalling abilities, MT1-MMP may …
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Comparative hypoxia responses and oxygen sensing in galaxiid fishes
… protein hypoxia inducible factor 1 (HIF-1), the effect of inescapable moderate hypoxia at 5 mg L-1 dissolved oxygen concentration (without access to the water surface) on these parameters was investigated. Swimming speed decreased significantly from 21.6 ± 2.6 to 7.4 ± 2.0 BL min-1 in …
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Normal Glycolytic Enzyme Activity Is Critical For Hypoxia Inducible Factor-1A Activity and Provides Novel Targets For Inhibiting Tumor Growth
… by the hypoxia-inducible factor 1 alpha (HIF-1α) transcription factor in states of low oxygen stress is a well described phenomenon <sup>[3, 4]</sup>. Through the use of a high throughput siRNA screen using an siRNA library targeting all the known open reading frames of the human genome …