Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"HES1"”.
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P107 negatively regulates the neural precursor pool by repressing Hes1 transcription
… in both the transcript and protein levels of Hes1 in p107-/- brains using in situ hybridization and western blot analysis. Examination of the Hes1 promoter revealed three putative E2F binding sites, which were subsequently found to bind E2F3 and E2F4 using chromatin immunoprecipitation. P107 …
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Impact du niveau d'activation de la voie Notch/HES1 dans le maintien du phénotype transformé des cellules pancréatiques cancéreuses
… L’expression aberrante de la protéine HES1, cible de la signalisation Notch, est observée dès les premières phases de la carcinogenèse (Miyamoto et al., 2003). L’utilisation d’inhibiteurs de gamma-sécrétase semble être une stratégie thérapeutique prometteuse soutenue par de nombreuses …
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Sampling Strategies for Characterization of Neuropeptides Using Mass Spectrometry and Functional Implications Into Cell -Cell Signaling
… pituitary were analyzed using transgenic mice. Hes1 KO is a genetically modified mouse that lives only e18.5 (embryonic days). Anterior pituitaries of Hes1 null mice exhibit hypoplasia due to increased cell death and reduced proliferation and in the intermediate lobe, the cells differentiate …
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The role of notch signaling in the development of the hypothalamic-pituitary axis and arcuate nucleus
… Additionally, the Notch signaling effector gene Hes1 is present in the developing hypothalamus and pituitary and is required for proper formation of the pituitary. Therefore, we hypothesized that Hes1 is necessary for the generation, placement and projection of AVP neurons. To test this …
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Genetic Control of Eye Development, a Case Study Focused on the Murine Mutation Belly Spot and Tail (BST)
… stalk fissure fusion, and the proneural gene Hes1, which has been shown to regulate retinal ganglion cell differentiation. We cross-mated hemizygous Bst/+ mutant mice with hemizygous Krd/+ (kidney and retinal defects, in which Pax2 is deleted) mice and Hes1 +/- knockout mice to produce Bst/+ …
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Notch signaling and cell cycle inhibitor regulation in pituitary organogenesis
… show that the activated Notch pathway, through HES1, serves as a proliferative signal that promotes progenitor expansion, with its loss resulting in a severely hypomorphic pituitary. HES1 transcriptional repression of Cyclin Dependent Kinase Inhibitors (CDKI) is the mechanism by which HES1 …
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Notch Signaling Inhibited By Ikaros1 In Human T-Cell Acute Lymphoblastic Leukemia
… in the promoter regions of Notch target genes, Hes1 and pTα.</p> <p>To determine whether this reciprocal regulation of Notch and Ikaros occurs in human T-ALL, we expressed active intracellular Notch receptors in human T-ALL cell lines and showed that Notch activation does not significantly …
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Mathematical modelling of negative feedback signal transduction processes
… we consider mathematical<br/>models of the Hes1, Hes1-Stat3 and p53-Mdm2 pathways.<br/>In chapter 3, we have undertaken a detailed study of the previous work done in the field. Building on this previous work, we derive mathematicalmodels (systems of partial differential equations) to capture …
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Sampling strategies for characterization of neuropeptides using mass spectrometry and functional implications into cell-cell signaling
… pituitary were analyzed using transgenic mice. Hes1 KO is a genetically modified mouse that lives only e18.5 (embryonic days). Anterior pituitaries of Hes1 null mice exhibit hypoplasia due to increased cell death and reduced proliferation and in the intermediate lobe, the cells differentiate …
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A Genomic Approach to Identify The Notch Pathway As A Putative Tumor Suppressor In Endometrial Cancer
… the ten genes validated, <em>HEYL</em> and <em>HES1</em>, are down-stream targets of the Notch signaling pathway. <em>HEYL</em> and <em>HES1</em> were identified by microarray and qRT-PCR to have a significant decrease in expression in endometrial carcinomas compared to normal endometrium. We …
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Mathematical modeling and sparse identification of delay-driven dynamical systems with applications to gene regulatory networks
… reaction–diffusion models motivated by the Hes1 gene regulatory system. A spatiotemporal framework is proposed to describe the interaction between Hes1 mRNA and protein, explicitly incorporating nuclear–cytoplasmic structure, diffusion, and transcriptional delay. Rigorous stability analysis …
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Transcriptional and Post-Translational Mechanisms Contribute to Maintenance of Rest In Neural Tumors
… evidence that the Hairy Enhancer of Split (HES1) protein represses <em>REST</em> transcription in medulloblastoma cell lines, modulates the expression of neuronal differentiation genes, and alters the survival potential of these cells <em>in vitro</em>.</p> <p>We also show that REST directly …
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Characterization of Notch1 and Pi3K-Pten-Akt/Mtor Pathway Interaction In Head and Neck Squamous Cell Carcinoma
… to upregulate PTEN activity in T-ALL cells via Hes1 downregulation (Palomero T, et al., 2007). Unfortunately, the mechanism of communication between the two pathways is unknown in HNSCC. Understanding the interaction between the NOTCH1 and PI3K/AKT/mTOR signaling pathways presents them as …
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Molecular And Therapeutic Implications Of Notch1 Signaling In Pediatric T-Cell Acute Lymphoblastic Leukemia
… in median transcripts for NOTCH1 target genes (HES1, DELTEX1 and cMYC). However, none of these mutations were associated with treatment outcome. Increased HES1, DELTEX1 and cMYC transcript levels were associated with significant increases in the transcript levels of several chemotherapy relevant …
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The Influence of Necrotic Enteritis, Environmental Factors, and Genetics on Intestinal Development Pathways and Disease Occurrence in Broiler Chickens
… innate immunity, and decreased transcription of Hes1, which is involved in Notch signaling towards absorptive cell differentiation. Hes1 has previously been shown to be involved in the inflammatory response and could be an area of interest in determining new treatments to prevent or relieve the …
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Invasion and Proliferation in Malignant Cells
… also observed a Notch1 independent activation of Hes1 in breast cancer. Further, postmenopausal patients with ERK1/2 phosphorylated tumors had an impaired tamoxifen response, but ERK1/2 phosphorylation was not linked to tamoxifen resistance in premenopausal women. Taken together, our results …
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A Developmental Pathway for Epithelial-to-Motoneuron Transformation in C. Elegans
… state. Down-regulation of the Notch-dependent Hes1 and Hes5 transcriptional inhibitors in differentiating cells induces expression of the proneural bHLH transcription factors Olig2 and Ngn2, leading to adherens junction loss, delamination from the epithelium, cell migration, and neuronal …
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Mechanistic Insights into the Notch signalling pathway through Molecular Condensation
… Live Notch target gene promoter reporter, called Hes1-Live-RNA, to allow for both the visualization and quantification of Notch activity within living cells. Using these tools, I discovered that the Notch 1 intracellular domain forms molecular condensates which in turn positively facilitates both …
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