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Showing 1 to 20 of 24 for “"HEPES"”.
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The Effects of Host-like Environmental Signals and Gene Expression on Capsule Growth in Cryptococcus neoformans
… air or 5%), cAMP (0 mM or 20 mM), and pH buffer (HEPES/no HEPES). We explore the effects of exogenous cAMP at a range of concentrations and of deletions of cAMP pathway genes <em>PKR1</em>, <em>PDE1</em>, and <em>PDE2</em>. We present a computational framework for identifying a set of genes that …
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ULTRARAPID FREEZING OF MOUSE EMBRYOS
… embryos. Upon comparing the use of phosphate-, Hepes- and bicarbonate-buffered T6 medium containing 3.5 M or 4.5 M DMSO and 0.25 M sucrose for cryopreservation, phosphate-buffered T6 containing 3.5 M DMSO was found to result in a reduced in vitro blastocyst formation rate (72 % ) compared to …
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Immunogenicity and Quantitation of Soluble Anaplasma Marginale Antigens From Cell Cultures and Blood
… with 10% adult bovine serum and 25 mM HEPES buffer. Better growth was observed in cultures initiated with lower percentage of parasitized erythrocytes (PPE) than those at higher PPE. Mineral supplementation (KCl and or MgSO(,4)) did not improve the ability of medium to support …
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Ultrastrukturelle Endothelveränderungen bei unterschiedlich konservierten Venentransplantaten - Konsequenzen für die Praxis
… physiologische Kochsalzlösung, Medium 199 + 20mM HEPES + 5% bovines Serumalbumin und Medium 199 + 20mM HEPES + 20% humanes Serumalbumin. Die Auswertung des Endothelzellschadens erfolgte mittels raster- und transelektronenmikroskopischer Untersuchungen sowie histopathologischer Aufarbeitung. …
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The viability and fertility of bovine spermatozoa encapsulated in microcapsules and microgels
… for bovine spermatozoa encapsulated in PIPES, HEPES, or saline microgels. Neat semen was pooled from five bulls (50 x 10° sperm/bull), encapsulated in alginate microgels, and incubated at 37 C for 8 h. The unencapsulated control displayed greater maintenance of viability for percent intact …
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Reproductive Biology of the Flat Floater Mussel, Anodonta Suborbiculata, Say, 1831 (Mollusca: Unionidae)
… to temperature, pH, PCO₂, and the presence of HEPES buffer. Mortality of glochidia in vitro was significantly higher at 10 °C than at 15 or 20 °C. Transformation success was significantly higher at lower levels of pH and higher levels of CO₂. However, reasonably high transformation success was …
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Nutrient Transporter targeted Delivery of HIV Protease Inhibitors
… Finally, the effect of common buffer component HEPES on the uptake and transport of P-gp substrates was carefully examined. It was found out that HEPES in the cell growth medium and experimental buffer may affect the ATP-dependent cellular influx and efflux processes, which may provide some …
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Studies on the internalization and intracellular transport of horseradish peroxidase in Chinese hamster ovary cells
… mannan (19 mg/ml), intravesicular pH elevators HEPES (40 mM) or monensin (10 μM), or substances which should deplete cellular ATP NaF/KCN (2 mM /1 mM), showed no inhibition of dissociation kinetics. A chase at 17℃ inhibited dissociation of HRP over the entire 30 minute period. This HRP binding …
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Steps towards successful cryopreservation of fish gametes: improving laboratory protocols in the Zebrafish model to increase gamete viability and efficiency
… in rainbow trout oviductal fluid (RTOF), RTOF + HEPES, or RTOF under a blood-gas atmosphere. Incubation in RTOF under a blood gas atmosphere significantly extended viability in both gametes to 24 hours. Second, I attempted to minimize the number of sperm necessary to fertilize a single egg by …
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Development of Multifunctional Nanoparticles for Cancer Therapy Applications
… containing lower ionic concentrations, HBSS and HEPES, using the original iron oxide nanoparticles compared to an iron oxide nanocomposite. The nanocomposite in the HEPES buffer displayed the greatest stability with a zeta potential of -30.47 mV and particle size of 155.4 nm. Stabilization of the …
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The effects of bicarbonate and mineral surfaces on uranium immobilization under anaerobic conditions
… and gamma-Al2O3. In the presence of a HEPES buffer at pH 8, results demonstrate direct homogeneous reduction in several hours in the absence of Fe-Gel or gamma-Al2O3, and reduction within a 48-hour period in the presence Fe-Gel or gamma-Al2O3. While adsorbed to both ferric and …
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Investigation of biological macromolecules using atomic force microscope-based techniques
… extracellular connexon surface in presence of HEPES, an aminosulfonate compound. At low pH (< 6.5), connexons showed a narrow and shallow channel entrance, which represented the closed pore. Increasing pH values resulted in a gradual opening of the pore, which was reflected by increasing …
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Interaction of Bdellovibrio Bacteriovorus and Host Bacteria
… with E. coli ML 35 (lac i-z*y ) in 0.025 M Hepes buffer, pH 7.8, supplemented with 0.002 M CaCl2*2H 0. Growth of Bdellovibrio occurred within a wide pH range, with the optimum being from 7.5 to 8.5. No growth occurred at pH 6.5. The broad pH range may occur because a buffer per se is not …
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Quantum dot labelled antimalarials and investigation of their interaction with synthetic haemozoin
… studies carried out in this lab, a 0.01 M HEPES (pH 7.5) buffer was used for all the experiments in order to afford a good solubility of Fe(III)PPIX and a minimal QD-COOH emission quenching. In order to use QD-COOHs to study the interactions of CQ with Fe(III)PPIX and β-haematin it was …
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Analysis of the Metabolism of Dextran-Methylprednisolone Succinate Using
… was accomplished by perfbsion of the liver with HEPES buffer containing collagenase, followed by centrihgation through a density gradient of Percoll to yield populations of hepatocytes (93% pure, with 80% viability) and Kupffer cells (98% pure, with 92% viability). Together, these experiments …
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Mechanism of Action of Pentobarbital Induced Attenuation of Vascular Smooth Muscle Contraction
… treatment of cells depolarized with 55 mM KC1 in HEPES buffered (pH 7.4) physiologic saline solution decreased ECI by 25% over the same temperature range. PUFA/PB combination had an additive inhibitory effect (45% decrease). These data suggest that PB may increase membrane fluidity, and thus …
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Postnatal Maturation Modulates Cerebrovascular Myogenic Tone
… MCA. Measurements were done in calcium replete HEPES buffer in the absence and presence of, cyclopiazonic acid (CPA) to deplete [Ca2+]i stores or with the calcium channel blocker, lanthanum (La ), or with La plus CPA. The results demonstrated that release of calcium from intracellular stores is …
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Development and In-Vitro Evaluation of Long Circulating Liposomes for Targeted Delivery of Gemcitabine and Irinotecan in Pancreatic Ductal Adenocarcinoma
… with an aqueous hydrating medium comprising of HEPES, Tween 80, and sodium chloride. This dispersion was subjected to freeze thawing and subsequent membrane extrusion. The liposomes were surface modified by incubating the liposomes with 5 mol% of PEG or poly(2-ethyl-2-oxazoline) conjugate and …
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Analyse von Single Nucleotide Polymorphisms an Glas-Oberflächen
… mit einer Konzentration von 250 ng/µl in einem HEPES-EDTA-NaCl-Puffer auf mit NeutrAvidin beschichtete Glasträger zu spotten. In den anschließenden Hybridisierungs- und Dissoziationsexperimenten bei 30 °C konnte die Diskriminierung von homocygoter Wildtyp- und homocygoter Mutanten- sowie …
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Nanoporous Calcium Carbonate-Based Substrates for the Controlled Delivery of Functional Materials
… (2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) buffer solution, and neither does anethole from ethanol. FCC also does not adsorb the cationic probe benzyltrimethylammonium bromide (BTMAB) or the anionic probe sodium 2-naphthalenesulphonate (Na2NS). However, it was found that vanillin …
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