Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"HEK293T CELLS"”.
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The role of sphingosine kinases and dihydroceramide desaturase in cell survival/apoptosis and inflammation pathways
… oxidative stress, and Mdm2 (E3 ligase) in HEK293T cells.;The polyubiquitinated forms of Degs1 acquire a 'gain of function' and activate pro-survival pathways, p38 MAPK/c-Jun N-terminal kinase (JNK), and X-box protein 1s (XBP-1s) in HEK293T cells. In contrast, the sphingosine kinase …
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THE ROLE OF P24 PROTEINS IN THE ER TO GOLGI TRANSPORT OF TOLL-LIKE RECEPTORS
… TLRs. Protein interaction studies performed in HEK293T cells by Proximity Ligand Assay (PLA) reveal that TMED7 interacts with TLR2 and TLR4, but not with TLR3. In contrast, TMED2 interacts with TLR2, TLR4 and TLR3. Different TLRs were co-expressed with ER-export deficient TMED7 and TMED2 and …
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Engineering the Nicotiana benthamiana secretory pathway for the production of Lujo virus vaccines and diagnostic tools
… this study we developed a production pipeline in HEK293T cells and N. benthamiana for the protein LUJV GP-C∆TM (Lujo virus glycoprotein precursor without the transmembrane region). The LUJV GP-C∆TM was constructed from gene sequences of the envelope glycoprotein of Lujo virus and then adapted to …
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Tet1: A Unique Dna Demethylase For Maintenance of Dna Methylation Pattern
… both in vitro and in mammalian cells. Based on their potent oxidative activities on 5mC, TET proteins have shown great potential as the long-sought DNA demethylases that induce DNA demethylation through multiple potential mechanisms. Here, we show that overexpression of TET1 …
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Identification of Novel CCR4 Antagonists Through Inhibition of CCL22-Induced Cell Migration
… of inflammatory mediators released by immune cells at the injury site and their impact on sensory neurons, thus contributing to the development and maintenance of pain. In previous studies, our group observed a significant increase in skin-resident dendritic cells (DCs) at the site of injury …
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Mutational and Structural Investigation of SARM1, a Protein that Mediates Axon Loss
… natural mutant. Mutants were expressed in HEK293T cells to determine their influence on NAD levels and mutant proteins isolated using immunoprecipitation for NADase assays of basal and NMN-induced activity. Selected ARM mutants and SARM1W253C were further characterised by microinjecting SCG …
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Repurposing Alzheimer’s disease medications in a drug combination for the study of the crosstalk between cell apoptosis and autophagy in the modulation of cell death in chronic myeloid leukaemia
… to be repurposed for the treatment of CML . HEK293T (cell model of AD) and K-562 (cell model of CML) cells were individually treated with the test drugs for 24 and 48 h, depending on the assay. Cell viability was assessed by overlaying the treated cells with CyQUANT® Direct assay dye for 40 …
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Methods to Probe the Function of Modified Bases in DNA
… sequencing is performed in SMUG1 knockdown HEK293T cells to determine the genomic location of 5-hmU in mammals. An increase in 5-hmU loci is observed upon SMUG1 knockdown. 5-hmU enriched regions are found to be T-rich and depleted in exons and promoters. Furthermore, 5-hmU sites show poor …
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TDP-43 Is Directed to Stress Granules by Sorbitol, a Novel Physiological Osmotic and Oxidative Stressor
… that directs TDP-43 to stress granules in Hek293T cells and primary cultured glia. We quantify TDP-43 association with stress granules over time and show that stress granule association and size are dependent on the glycine-rich region of TDP-43, which harbors the majority of pathogenic …
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Contribution of Causal Aquaporin-1 Mutations to the Pathobiology of Pulmonary Arterial Hypertension
… mutant AQP1 proteins were overexpressed in Hek293T cells, then subjected to confocal microscopy and membrane biotinylation followed by biotin-based ELISAs, to investigate whether the mutant proteins are able to traffic to the cellular membrane. These studies suggested normal membrane …
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Exploring the link between RAG1 Cellular Distribution and Components of the CRL4DCAF1 E3 Ligase
… for regulating RAG1 localization within HEK293T cells. Treating transfected cells expressing RAG1 with inhibitors of the ubiquitin- proteasome pathway did not change RAG1 localization during a time-lapse. The distribution pattern of cells transfected with full-length RAG1 (FLRAG1) or core …
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Engineering a microneedle patch for opioid overdose therapy
… fluorescence imaging of fentanyl-treated HEK293T cells demonstrated functional naloxone antagonism, as evidenced by a time-dependent reduction in opioid-induced fluorescence. This study highlights GelMA-based microneedles as a robust and adaptable platform for opioid antagonist delivery. …
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Angiomotin Is A Novel Cadherin-11 Interacting Protein That Mediates Migration In Prostate Cancer Cells
… Furthermore, Cad11 expression endows PCa cells with increased migratory potential and metastasis to bone. Deletion of the Cad11 cytoplasmic region (cyto) resulted in loss of cell migration. How the Cad11 cytoplasmic domain mediates cell migration is unknown. We have identified angiomotin …
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Engineering Tools to Promote and Characterize Wnt-Mediated Stem Cell Differentiation
… time course data derived from Western blots of HEK293T cells treated with Wnt. From this fit we were able to gain insights into how the intracellular levels of the Wnt pathway component β-catenin are regulated. To better characterize the downstream effects of Wnt signaling during the …
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Protein Factors Regulating Mitochondrial Respiratory Supercomplexes
… models of each HIG1 protein were generated in HEK293t cells to preform comparative studies. We uncover a general dichotomy in the effects observed from HIGD2A vs. HIGD1A/1B/1C OE. Furthermore, we demonstrate that the previously unstudied protein family member HIGD1C is a negative regulator of …
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Interrogating regulated intramembrane proteolysis of IGF-1R, and its role in cell migration
… RIP was thought to be a means by which cells would terminate receptor signalling, however contemporary research indicates a more nuanced role. It is now understood that the fragments generated through RIP can retain signalling of the full-form receptors and, in some instances, may …
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Characterization of the zebrafish zipper interacting protein kinase homolog
… were created and used for transfecting into Hek293T cells and Hela Cells. From these transfections, cell counterstaining and confocal microscopy we found that ZIPK is expressed ubiquitously throughout the cell, although mainly cytoplasmic. To study the effects on cell shape various ZIPK …
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Regulation of mGluR7 trafficking by SUMOylatio in neurons
… conjugation of full-length mGluR7 in mammalian cells and neurons. Here we have explored whether mGluR7 is a target of SUMOylation. Using biochemical approaches coupled with confocal imaging, we find that mGluR7 at Lys889, the sole SUMOylation site on the C-terminus of mGluR7 is a target of SUMO …
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Konstruktion und Charakterisierung von rekombinanten Immuntoxinen zur Therapie von metastasierendem Pankreaskarzinom
… was calculated to be 0,0075 µg/ml for L3.6pl cells and 0,008 µg/ml for FG cells, respectively. Furthermore the anti-tumor effect of this recombinant immunotoxin was confirmed using a disseminated human pancreatic cancer nude mouse model. After intravenous injection of L3.6pl cells into …
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Role of plasma membrane ATPase4 in the pathophysiology of pulmonary arterial hypertension
… apoptosis of pulmonary arterial endothelial cells (PAECs). Elevated levels of pro- inflammatory cytokines such as TNF-α are a hallmark of PAH and are known to induce endothelial injury. Calcium dysregulation contributes to apoptotic signalling, suggesting that calcium transporters like Plasma …
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