Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 87 for “"HEK-293"”.
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Expression and Function of Alpha3 and Beta2 Neuronal Nicotinic Acetylcholine Receptor Subunits in HEK-293 Cells
… from the human embryonic kidney cell line 293 at mRNA levels found in the CA1. The functional properties of α3β2 in HEK-293 cells were characterized by whole-cell patch clamping using acetylcholine (ACh) as an agonist. The kinetics of α3β2 channels were further analyzed by altering the …
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Effect of Thyroid Hormones on AT1R MRNA Levels in HEK 293 Cells: Implications for Early-Onset Hypertension in Children Born of Preeclamptic Women
… mRNA levels in human embryonic kidney cells (HEK). Maternal preeclampsia is a prediction of higher ambulatory blood pressure (ABP) in the exposed offspring. The mother's blood pressure affects the blood pressure of the fetus since the placenta mediates blood flow. Thyroid hormone can increase …
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The Cystic Fibrosis Transmembrane Conductance Regulator: Regulation by HSP-90
… of CFTR from stably transfected HEK-293 cells, electrophoretic separation of cross-linked proteins, and protein identification via SDS-PAGE, western blotting, and mass spectrometry. Also, this hypothesis is tested by (b) functionally assessing the effect of Hsp90 on CFTR function …
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Lectina coagulante de Moringa oleifera (cMoL) : citotoxicidade, toxicidade dérmica, efeito anti-infeccioso in vivo e incorporação em membrana a base de Galactomanana
… a base de galactomanana contendo cMoL. Células HEK-293 foram incubadas por 24 horas com cMoL (3,125–100 μg/mL). No ensaio de toxicidade dérmica, os camundongos tratados topicamente com dose única (200 μg/mL) de cMoL foram analisados por 14 dias. O ensaio de atividade anti-infecciosa de cMoL (100 …
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Estudo do modo de interação do fármaco cloroquina com o DNA e seu efeito em células
… tipos distintos de linhas celulares normais, HEK-293 (células renais humanas), melan-a (células epiteliais murina) e VERO (células renais de primata), que permite avaliar e comparar os efeitos do fár- maco na viabilidade celular. A citotoxicidade é um dos indicadores mais importantes de …
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Molecular mechanisms of cadmium toxic
… (human hepatoma), 1321N1 (human astrocytoma) and HEK 293 (human embryonic kidney) cell lines after 24hrs exposure to 5, 10 and 50 μM cadmium chloride (CdCl₂). The role of the Nrf2-Keap1-ARE pathway in the adaptive response of these cell lines to Cd exposure was examined, and the possible …
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Régulation du récepteur aux opioïdes de type delta par les protéines Homer1
… for this interaction. Indirect ELISA assays in HEK 293 cells resulted in a decrease in hDOP cell-surface expression with transient overexpression of Homer1b; preliminary results showed also a decrease of hDOP cellsurface expression with CT 339 and 354 deletions. Furthermore, endogenous Homer1b …
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Beteiligung der Phospholipase D an der Regulation der Synthese von Phosphatidylinositol-4,5-bisphosphat durch Arf- und Rho-GTPasen
… PIP-5-kinase. Overexpression of PLD isoforms in HEK-293 cells led to an increase in PIP-5-kinase activity and to elevated PIP2 levels in intact cells. As both PLD and PIP-5-kinase are stimulated by the GTPases Arf1 and RhoA, we investigated in the following, if PLD is involved in the regulation …
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Phosphoglycerate kinase 1 as a therapeutic target in motor neuron disease
… I demonstrate that increased PGK1 activity in HEK-293 cells is well tolerated and does not result in the upregulation or downregulation of any cellular pathways supporting the further exploration of PGK1 agonists as potential future therapeutics. A metabolic assay in HEK-293 cells was used to …
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An investigation into the molecular mechanisms underlying retinitis pigmentosa 17 with the view to developing novel gene- based therapies
… in the human embryonic kidney cell line, HEK-293, strikingly different results were obtained. These cells were unaffected by the expression of the R14W mutant CAIV and were able to process the mutant and WT protein equally effectively. These findings regarding cell type specificity were …
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FHL2 Inhibits Calcineurin and Represses Pathological Cardiac Hypertrophy
… by calcineurin-NFAT-dependent transcription, HEK 293 cells were transfected with luciferase reporter constructs containing the NFAT-driven promoters of either RCAN1 or IL-2. Consistent with the in vivo data, knockdown of FHL2 message using siRNA led to increases in both RCAN1 and IL-2 promoter …
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An electrophysiological study of the effects of resveratrol and catechin at GABAa receptors
… GABA<sub>A</sub> receptors expressed in HEK-293 cells using electrophysiological techniques.<.p><p>HEK-293 cells were cultured and transfected using eDNA encoding human GABA<sub>A</sub> receptors. GABA-evoked currents were recorded from HEK cells 24-48 hours following transfection. Cells …
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THE ROLE OF THE PROLINE-RICH TYROSINE KINASE 2 PROTEIN IN THE REGULATION OF METABOTROPIC GLUTAMATE RECEPTOR SIGNALLING
… kinases (ERKs) by Group I mGluRs. In HEK 293 cells, ERK1/2 phosphorylation is greater in cells co-expressing mGluRl and wild-type Pyk2 than in cells expressing the receptor alone, and blocked in cells co-expressing the receptor and a dominant-negative mutant Pyk2. In cortical neurons, …
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Defining the functional response of Mycobacterium sp. infected macrophages to structural variants of Interferon gamma (IFN-y)
… and expressed in both Escherichia coli and HEK 293 cells. The resulting proteins were purified and analysed using circular dichroism (CD) and intrinsic fluorescence spectroscopy to probe secondary and tertiary structural organisation. This approach enabled direct comparison of structural …
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Design, Synthesis, and Evaluation of N-Methylated H2R2W4
… and ~60% against normal human kidney cells (HEK-293) at the concentration of ≥80 μg/mL. Cytotoxicity and stability of AMPs is their clinical limitation. Therefore, we hypothesized that <em>N</em>-methylation strategy, specifically at the peptide backbone would modulate their cytotoxicity and …
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Solid phase peptide synthesis and TLR-5 activity analysis of pertide fragments from the Salmonella muenchen flagellin protein
… and their activity against TLR-5 expressing HEK-293 cells. Our goal was to resolve the minimal region of flagellin necessary to bind and/or activate TLR-5. Results showed significant agonist activity (P<0.01) with peptide N2-b (LAVQSANGTN), and peptide N2-f (LAVQSANGTNSQ). Peptide N2-c …
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Hybrid Cyclic-Linear Cell-Penetrating Peptides Containing Alternative Positive and Hydrophobic Residues as Molecular Transporters
… human epithelial embryonic kidney healthy (HEK-293), and human epithelial mammary gland adenocarcinoma cells (MDA-MB-231) using the MTS assay. The peptides did not exhibit any significant cytotoxicity at the concentration of 10 μM in CCRF-CEM, HEK-293, MDA-MB-231, and SK-OV-3 cells after 3 h …
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Nuclear Export Signal Recognition by CRM1 Carrying the Oncogenic E571K Mutation and Structure-Based NES Prediction
… cells, I used CRISPR/Cas to generate generated HEK 293 cells with either monoallelic CRM1WT/E571K or biallelic CRM1E571K/E571K. HEK 293 cells with CRM1WT/E571K or CRM1E571K/E571K had decreased proliferation and cells with homozygous CRM1E571K/E571K had obvious cell cycle defects. The …
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Developing Fluorescent Tools to Dissect the Role of Tuberin at Mitotic Onset
… Cyc B1 GFP tagged CRS variants and a fluorescent HEK-293 cell line which contains an iRFP 720 tag inserted into the genomic DNA at the amino terminus and carboxy terminus of Tuberin. These tools will be fundamental in studying the formation of this protein complex and the role of Tuberin in other …
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