Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 48 for “"HEK 293 Cells"”.
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Expression and Function of Alpha3 and Beta2 Neuronal Nicotinic Acetylcholine Receptor Subunits in HEK-293 Cells
… nAChR subtype α3β2 was transiently expressed in cells derived from the human embryonic kidney cell line 293 at mRNA levels found in the CA1. The functional properties of α3β2 in HEK-293 cells were characterized by whole-cell patch clamping using acetylcholine (ACh) as an agonist. The kinetics of …
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Effect of Thyroid Hormones on AT1R MRNA Levels in HEK 293 Cells: Implications for Early-Onset Hypertension in Children Born of Preeclamptic Women
… 1 receptor mRNA levels in human embryonic kidney cells (HEK). Maternal preeclampsia is a prediction of higher ambulatory blood pressure (ABP) in the exposed offspring. The mother's blood pressure affects the blood pressure of the fetus since the placenta mediates blood flow. Thyroid hormone can …
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Régulation du récepteur aux opioïdes de type delta par les protéines Homer1
… for this interaction. Indirect ELISA assays in HEK 293 cells resulted in a decrease in hDOP cell-surface expression with transient overexpression of Homer1b; preliminary results showed also a decrease of hDOP cellsurface expression with CT 339 and 354 deletions. Furthermore, endogenous Homer1b …
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Phosphoglycerate kinase 1 as a therapeutic target in motor neuron disease
… pathway. PGK1 may have multiple other roles in cells apart from its role in glycolysis but these are less well understood, and the consequences of increased PGK1 activity in cells and therefore its suitability as a therapeutic target is not known. Overexpression of PGK1 has been shown to be …
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FHL2 Inhibits Calcineurin and Represses Pathological Cardiac Hypertrophy
… by calcineurin-NFAT-dependent transcription, HEK 293 cells were transfected with luciferase reporter constructs containing the NFAT-driven promoters of either RCAN1 or IL-2. Consistent with the in vivo data, knockdown of FHL2 message using siRNA led to increases in both RCAN1 and IL-2 promoter …
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An electrophysiological study of the effects of resveratrol and catechin at GABAa receptors
… GABA<sub>A</sub> receptors expressed in HEK-293 cells using electrophysiological techniques.<.p><p>HEK-293 cells were cultured and transfected using eDNA encoding human GABA<sub>A</sub> receptors. GABA-evoked currents were recorded from HEK cells 24-48 hours following transfection. Cells …
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THE ROLE OF THE PROLINE-RICH TYROSINE KINASE 2 PROTEIN IN THE REGULATION OF METABOTROPIC GLUTAMATE RECEPTOR SIGNALLING
… kinases (ERKs) by Group I mGluRs. In HEK 293 cells, ERK1/2 phosphorylation is greater in cells co-expressing mGluRl and wild-type Pyk2 than in cells expressing the receptor alone, and blocked in cells co-expressing the receptor and a dominant-negative mutant Pyk2. In cortical neurons, …
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Defining the functional response of Mycobacterium sp. infected macrophages to structural variants of Interferon gamma (IFN-y)
… and expressed in both Escherichia coli and HEK 293 cells. The resulting proteins were purified and analysed using circular dichroism (CD) and intrinsic fluorescence spectroscopy to probe secondary and tertiary structural organisation. This approach enabled direct comparison of structural …
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Solid phase peptide synthesis and TLR-5 activity analysis of pertide fragments from the Salmonella muenchen flagellin protein
… and their activity against TLR-5 expressing HEK-293 cells. Our goal was to resolve the minimal region of flagellin necessary to bind and/or activate TLR-5. Results showed significant agonist activity (P<0.01) with peptide N2-b (LAVQSANGTN), and peptide N2-f (LAVQSANGTNSQ). Peptide N2-c …
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The Cystic Fibrosis Transmembrane Conductance Regulator: Regulation by HSP-90
… of CFTR from stably transfected HEK-293 cells, electrophoretic separation of cross-linked proteins, and protein identification via SDS-PAGE, western blotting, and mass spectrometry. Also, this hypothesis is tested by (b) functionally assessing the effect of Hsp90 on CFTR function …
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Nuclear Export Signal Recognition by CRM1 Carrying the Oncogenic E571K Mutation and Structure-Based NES Prediction
… CRM1 is essential to the survival of eukaryotic cells and is overexpressed or mutated in a variety of cancers. This involvement of CRM1 in cancer cell survival has led to the development of novel small molecule inhibitors such as XpovioTM or Selinexor, which has been approved for treatment of …
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Exploring the molecular mechanisms of AMPK-dependent regulation of neuroexcitability
… to oxygen sensing in carotid body type 1 cells, where hypoxia-induced inhibition of TASK-3 and/or TASK-1/3 potassium currents leads to voltage-gated calcium entry, exocytotic transmitter release and increases in carotid body afferent input responses that initiate corrective changes in …
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Molecular mechanisms of cadmium toxic
… evidence about its mechanism(s) of action in the cells. The present study was therefore undertaken with the aim of defining the role of oxidative stress, intracellular Ca²⁺ alteration via phospholipase C- Inositol-1,4,5-triphosphate (PLC-IP3) and mitochondrial-cytochrome c dependent pathways in …
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The role of latent membrane protein 2 in the pathogenesis of Epstein-Barr virus associated malignancy
… a lifelong latent infection in host B cells and is associated with the development of various lymphoid and epithelial malignancies. Approximately half of Hodgkin's lymphomas (HL) are EBV positive where LMP2 is highly expressed. However, the contribution of LMP2 to the pathogenesis of HL …
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Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery
… efficiency in breast and ovarian cancer cells. Therefore, the oleyl conjugated (WRH)<sub>n</sub> (n=1-4) peptides were synthesized using Fmoc/tBu solid-phase peptide chemistry, followed by purification using Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC), and …
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SYNTHESIS OF PHARMACOLOGICALLY ACTIVE MOLECULES THROUGH BIOCATALYTIC APPROACHES: STUDY OF THEIR STEREOCHEMISTRY AND POLYMORPHISM BY MEANS OF SPECTROSCOPIC AND CRYSTALLOGRAPHIC METHODS.
… biological tests are currently underway on HEK-293 cells expressing the human GnRH receptor (hGnRH1R) to verify the good receptor affinity predicted by the molecular dynamics simulations.
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Function and pharmacology of TRPM3 ion channel
… identified. Human TRPM3 was first expressed in HEK 293 cells and shown to be activated by hypo-osmotic challenge or sphingosine, consistent with previous reports. In addition, TRPM3 was activated by pregnenolone sulphate. Hydrophobicity analysis of the TRPM3 amino acid sequence revealed a short …
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Voltage Sensing Mechanism in the Voltage-gated and Proton (H+)-selective Ion Channel Hv1
… under whole-cell voltage clamp in transfected HEK-293 cells. Consistent with previous studies in VS domain containing proteins, we find that Hv1 R205H mediates a robust resting-state H+ ‘shuttle’ conductance (GSH) at negative membrane potentials. Voltage-dependent GSH gating is measured at more …
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TRP75-Mediated STAT3 Activation Promotes Anti-Apoptotic Signaling and Ehrlichia chaffeensis Infection
… infection. Ectopic expression of TRP75 in HEK-293 cells induced STAT3 phosphorylation, demonstrating a specific role for TRP75 in STAT3 activation. Collectively, these findings support a model in which E. chaffeensis exploits STAT3 via the TRP75 effector to activate an anti-apoptotic …
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Beteiligung der Phospholipase D an der Regulation der Synthese von Phosphatidylinositol-4,5-bisphosphat durch Arf- und Rho-GTPasen
… PIP-5-kinase. Overexpression of PLD isoforms in HEK-293 cells led to an increase in PIP-5-kinase activity and to elevated PIP2 levels in intact cells. As both PLD and PIP-5-kinase are stimulated by the GTPases Arf1 and RhoA, we investigated in the following, if PLD is involved in the regulation …
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