Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 169 for “"HEK"”.
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Expression and Function of Alpha3 and Beta2 Neuronal Nicotinic Acetylcholine Receptor Subunits in HEK-293 Cells
… in the CA1. The functional properties of α3β2 in HEK-293 cells were characterized by whole-cell patch clamping using acetylcholine (ACh) as an agonist. The kinetics of α3β2 channels were further analyzed by altering the level of α3 DNA transfected into HEK-293 cells. Varying the α3 concentration …
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Effect of Thyroid Hormones on AT1R MRNA Levels in HEK 293 Cells: Implications for Early-Onset Hypertension in Children Born of Preeclamptic Women
… mRNA levels in human embryonic kidney cells (HEK). Maternal preeclampsia is a prediction of higher ambulatory blood pressure (ABP) in the exposed offspring. The mother's blood pressure affects the blood pressure of the fetus since the placenta mediates blood flow. Thyroid hormone can increase …
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Multidrug resistance-associated protein 2 (MRP2) and oxaliplatin transport
… used for studies of oxaliplatin transport. A HEK293 cell line stably transfected to over-express the human ABCC2 gene (HEK-MRP2 cells) and its isogenic HEK293 parenteral (HEK-P) cell line, and a panel of seven human gastrointestinal cancer cell lines, were used to study the role of MRP2 in …
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A rádiófrekvenciás sugárzás biológiai hatásának vizsgálata emlős sejtekben
… intenzitásbeli változást vizsgáltunk HELA és HEK sejteken lézer-scanning citométerrel, továbbá áramlási citométer segítségével. A vizsgálataink során kapott eredmények igazolják, hogy a HEK és HeLa sejtek, egyaránt mutatnak γ-H2AX intenzitást RF besugárzás hatására, azonban különböző …
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The Cystic Fibrosis Transmembrane Conductance Regulator: Regulation by HSP-90
… of CFTR from stably transfected HEK-293 cells, electrophoretic separation of cross-linked proteins, and protein identification via SDS-PAGE, western blotting, and mass spectrometry. Also, this hypothesis is tested by (b) functionally assessing the effect of Hsp90 on CFTR function …
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Rutas de transducción de señales y procesos de fosforilización implicados en la regulación de canales erg por TRH
… receptor de TRH de forma endógena, como células HEK-H36/T1 que expresan estos dos elementos de forma heteróloga. En ambos casos, la adición de la TRH a las células provoca una rápida modulación de carácter inhibitorio sobre las corrientes erg. En primer lugar, en cuanto a los componentes de la …
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Lectina coagulante de Moringa oleifera (cMoL) : citotoxicidade, toxicidade dérmica, efeito anti-infeccioso in vivo e incorporação em membrana a base de Galactomanana
… a base de galactomanana contendo cMoL. Células HEK-293 foram incubadas por 24 horas com cMoL (3,125–100 μg/mL). No ensaio de toxicidade dérmica, os camundongos tratados topicamente com dose única (200 μg/mL) de cMoL foram analisados por 14 dias. O ensaio de atividade anti-infecciosa de cMoL (100 …
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Identifying Potential Agonists Of TRPA1 Using A Heterologous Expression System And Transgenic Mice
… FLUO-3AM, intracellular calcium levels of naive HEK and hTRPA1-HEK cells were monitored after exposure to a stimulus of interest. If a stimulus activated TRPA1, intracellular calcium levels increased resulting in increased fluorescence. Using the TRPA1 inhibitor HC-030031, elicitation of …
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Exploring the Functional Interaction Between CaMK-II and p53
… First, a stable p53 knockdown human cell line (HEK) was established through lentiviral transduction of p53 shRNA and verified with immunoblots and immunostaining assays. Next, the localization of CaMK-II and the cell growth rate in these cells was determined. In wild type HEK cells, …
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Growth Factors Up-Regulate Epithelial Stem Cells: Treatment Strategies for Colorectal Cancers
… Surprisingly, overexpression of autocrine PG in HEK-293 cells, imparted tumorigenic/metastatic potential to the cells (chapter 3). Based on these data, the second goal was to investigate the phenotypic differences between non-transformed and transformed stem cell using non-tumorigenic (HEK-C) and …
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An electrophysiological study of the effects of resveratrol and catechin at GABAa receptors
… GABA<sub>A</sub> receptors expressed in HEK-293 cells using electrophysiological techniques.<.p><p>HEK-293 cells were cultured and transfected using eDNA encoding human GABA<sub>A</sub> receptors. GABA-evoked currents were recorded from HEK cells 24-48 hours following transfection. Cells …
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Interactions between IP3 receptor and Kras-induced actin-interacting protein
… similarly with native and over-expressed KRAP in HEK cells. Mutation of a conserved motif (204FF) within KRAP (FFAAKRAP) disrupted the KRAP-IP3R interaction. Cytosolic free [Ca2+] measurements in HEK cells expressing individual IP3R subtypes indicated that KRAP licenses all three subtypes to …
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DEVELOPMENT OF A 3D ZWITTERIONIC HYDROGEL SYSTEM FOR CELL CULTURE AND EXTRACELLULAR VESICLES
… EV-producing cells like human embryonic kidney (HEK) cells and neutrophils has not been extensively explored due to challenges such as limited hydrolytic stability, inadequate functionality, and inefficient synthesis processes.[2] In this study, we introduce an efficient method for synthesizing …
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Untersuchungen zur Heteromerisierung, Funktion und genetischen Struktur von HCN Schrittmacherkanälen
… untersucht. Sowohl im Mausgehirn wie auch in HEK Zellen können die HCN Kanal-Untereinheiten miteinander interagieren. Im Mausgehirn wurde die Heteromerisierung von HCN1 und HCN2 durch Ko-Immunpräzipitation nachgewiesen. Durch konfokalmikroskopische Studien an EGFP- bzw. RFP-markierten HCN …
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Estudo do modo de interação do fármaco cloroquina com o DNA e seu efeito em células
… tipos distintos de linhas celulares normais, HEK-293 (células renais humanas), melan-a (células epiteliais murina) e VERO (células renais de primata), que permite avaliar e comparar os efeitos do fár- maco na viabilidade celular. A citotoxicidade é um dos indicadores mais importantes de …
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High-resolution optical analyses of IP3-evoked Ca2+ signals
… However, for analyses of Ca2+ puffs, I used HEK cells that had been engineered, using CRISPR/Cas9 to disrupt endogenous genes, to express single IP3R subtypes. Local Ca2+ signals evoked by flash-photolysis of caged- IP3 were recorded using Cal520 and total internal reflection fluorescence …
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Molecular mechanisms of cadmium toxic
… (human hepatoma), 1321N1 (human astrocytoma) and HEK 293 (human embryonic kidney) cell lines after 24hrs exposure to 5, 10 and 50 μM cadmium chloride (CdCl₂). The role of the Nrf2-Keap1-ARE pathway in the adaptive response of these cell lines to Cd exposure was examined, and the possible …
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The role of the E2 copper binding domain in the cell biology of the amyloid precursor protein
… to alanine. These constructs were expressed in HEK and SH-SY5Y cells and the effects of the mutations on the afore mentioned properties of APP were assessed. The results show that these mutations had little consistent effect on the expression, proteolysis or cellular localisation of APP. …
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The Lipid-Facing M4 Helix of α4β2 nACh and 5-HT3A Receptors
… characterisation of mutant receptors in HEK293 cells and Xenopus oocytes, and radioligand binding and immunofluorescence to assess ligand binding and receptor expression. I show that 1 out of 28 alanine mutations in the 5-HT3AR M4 and 8 out of28 double alanine mutations in the α4β2 nAChR …
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