Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"HEI-OC1"”.
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The Role of Connexin43 and Gap Junctional Intercellular Communication in the Apoptotic Process of Auditory Cells
… was to determine the role of gap junctions and their constituents, hemichannels and Cxs in the cisplatin-induced ototoxic mechanism. As a result, I found that inhibition of gap junctional activity with either Cx43 siRNA transfection or a pharmacological drug, 18α-glycyrrhetinic acid in HEI-OC1 …
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The Distribution of Innate Antioxidant Enzymes in Rat Cochleas
… turns and hair cell rows using ImageJ？？ program. HEI-OC1 cells were cultured and treated with 0.4mM GM for 48h. These cells were immunostained with antibodies against GSH, Cu/Zn-SOD, or Mn-SOD with GM antibodies. The expression of antioxidant enzymes was evaluated with a fluorescence microscope. …
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Gingko biloba extracts protect auditory hair cells from cisplatin-induced ototoxicity by inhibiting perturbation of gap junctional intercellular communication
… apoptosis and disturbance of GJIC. HEI-OC1 auditory cells were cultured and treated with cisplatin (50μM) and EGb (300μg/ml) for 24 h, and then analyzed by immunocytochemistry (Annexin V/ propidium iodide) and Western blots. The function of GJIC was evaluated by scrape-loading dye …
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Protection effect of EC on radiation-induced cell death of auditory cell in vitro and in vivo
… Epicatechin(EC)의 효과에 대해 확인하고자 하였다. 먼저 청각와우세포인 HEI-OC1 세포로 세포 생존률을 측정하였으며 annexin-V와 PI로 이중염색을 시행한 뒤 FACS를 시행하여 세포 고사율을 확인하였으며, 미토콘드리아 막 투과성의 변화와 활성산소의 변화를 측정하였다. 또한 TUNEL assay와 투과 전자 현미경을 사용하여 세포고사를 현미경하에서 관찰하였다. 또한 기전을 확인하기 위하여 Western blot을 시행하였으며, caspase 3 활성도도 측정하였다. 생체 실험으로는 …
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CANNABINOID RECEPTORS (CB) IN COCHLEA: CHARACTERIZATION AND OTOPROTECTIVE FUNCTIONS
… glycerol (2-AG), enzymes involved in their biosynthesis and degradation such as diacylglycerol lipase-α (DAGL- α), and CB receptors. Primarily, there are three types of CB receptors - CB receptor 1(CB1), CB receptor 2 (CB2) and non CB1 non CB2 types of CB receptor (e.g. GPR, TRPV1) and …