Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"HDX-MS"”.
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Application of hydrogen deuterium exchange mass spectrometry in protein-ligand and protein-protein interactions
… (EPR) spectroscopy, and mass spectrometry (MS). Compared with NMR and EPR spectroscopy, MS has less stringent sample requirements, including protein concentration and protein size. Moreover, the mass accuracy, sensitivity, and faster data analysis also have contributed to the rapid growth of …
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Advancing the characterization of glycan dynamics with in-electrospray ionization hydrogen/deuterium exchange-mass spectrometry.
… exist to study carbohydrates, mass spectrometry (MS) remains one of the most widely used methods because it provides structural information. Hydrogen/deuterium exchange (HDX-MS) is a labeling technique that monitors the exchanging labile hydrogen atoms for molecules across different timepoints. …
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Biophysical Investigation into the Protein Dynamics Governing the Allosteric Regulation of Plant and Animal 15-Lipoxygenases
… attacks. There are also multiple isoforms present in humans, which have contradictory roles in the body. Specifically, human 15-LOX isoforms, 15-LOX-1 and 15-LOX-2, are involved in both homeostasis and pro-inflammatory pathways. In order to selectively target the activity of these …
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Investigating the Structure and Physical Stability of Glp1-Like Peptides Using Mass Spectrometry
… batch of comparable purity. Mass spectrometry (MS) is an increasingly important tool in biopharmaceutical characterisation, with great potential for understanding the conformational bias of inherently dynamic systems like fibrillating peptides. Two complementary structure based MS techniques are …
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Investigating the Rotary Mechanism of ATP Synthase Using Molecular Dynamics Simulations
… opening events coincided with experimental HDX patterns obtained in our laboratory. HDX-MS data suggests that in vitro operation of F1-ATPase is associated with significant rotational resistance in the apical bearing. These conditions cause the γ C-terminal helix to get “stuck” while the …
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Structural Basis of Signal Transduction within Environmental Sensing PAS Regulated Ser/Thr Kinases
… biochemical and biophysical tools, including HDX-MS, cryo-EM, NMR, and X-ray crystallography to elucidate the structural dynamics of these proteins both at the domain and multi-domain levels.</p> <p>In hPASK, our high-pressure NMR and HDX-MS data with artificial ligands identified ligand …
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Structural Polymorphism of Alpha-Synuclein in Conditions Resembling the Cellular Environment
… disease with motor and non-motor symptoms that impacts the patients’ everyday function and severely affects their quality of life. Current treatments are symptomatic and do not target the cause and molecular mechanism of the disease, which remains elusive. Evidence points towards the …
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Study of the Dynamics of Retaining GT-B Fold Glycosyltransferases Using Hydrogen Deuterium Exchange Mass Spectrometry
… hydrogen-deuterium exchange mass spectrometry(HDX-MS) is used to analyze CgMshA from Corynebacterium glutamicum, a model enzymefrom GT-B fold. In the presence of acceptor substrate inositol-1-phosphate (I1P), decreaseddynamics occur in CgMshA's N-terminal domain (NTD) and linker regions. …
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Actin-like filament A (AlfA) and AlfA-driven plasmid segregation
… hydrogen-deuterium exchange mass spectrometry (HDX-MS). A comparison of AlfA structure with other actins revealed a unique nucleotide binding conformation, which causes AlfA to remain stable after nucleotide hydrolysis. In addition to the filament structure, we reconstituted the segresome …
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Structural and Functional Characterisation of the Nutrient Sensing Kinase GCN2
… degeneration and cancer. The molecular mechanisms that control the regulation and activation of GCN2 remain unclear. Some insights have been provided through genetic experiments on yeast, but the complexities of the regulatory pathways have made it difficult to decipher precise mechanistic …
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Mass Spectrometry-Based Strategies for Biomolecular Structure Analysis: Steroid Metabolites Structures and Protein-Protein Interactions
… and proteomics, and ongoing improvement in MS-based techniques, mass spectrometry is extensively applied in the study of both small and large molecules.The research presented in this thesis falls into two main parts, which focus on the application of MS in (1) structural analysis of steroid …
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Characterisation of Prodomains in the GDNF Family
… on signalling, the different protein forms were evaluated using cell-based bioactivity assays. Both uncleaved pro-NRTN and pro-GDF15 have reduced RET signalling activity in comparison to their mature domains alone. However, cleaved pro-NRTN becomes as active as mature NRTN. Pro-GDF15 …
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Hydrogen-deuterium exchange studies by mass spectrometry: A method for obtaining conformational structures of proteins in solution
Hydrogen-deuterium exchange (HDX) coupled with electrospray ionization (ESI) in mass spectrometry (MS) has become an important method in the study of protein structure and dynamics. It provides an opportunity for obtaining conformational structures of protein motions in physiological conditions and …
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FROM ANTIBODY-DRUG CONJUGATES TO MASKED ANTIBODIES: BIOPHYSICAL INSIGHT FOR THE RATIONAL DESIGN OF FUTURE THERAPIES
… how the additional disulfide bridge forms, interconverts, and changes the stability and structural dynamics of the antibody. Alternative strategies to the use of maleimide linkers have recently been developed, e.g. DVP and tetraDVP linkers, by the Spring Group in the Department of …