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Showing 1 to 7 of 7 for “"HCoV-NL63"”.
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A Study of Human Coronavirus NL63: Characterization of Virus Replication, Targeting of Papain-Like Protease 2 by Antivirals and Probing Virus Mediated Innate Immune Antagonism
… the replication of human coronavirus NL63 (HCoV-NL63) in primary human airway epithelial cultures and characterized the activity of the viral papain-like protease 2 (PLP2) domain. Using quantitative real time PCR and imaging techniques I showed that HCoV-NL63 undergoes productive …
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Cloning and expression of the M-gene from the human coronavirus NL-63 in different expression systems
… of two additional novel human coronaviruses (NL63 and HKU1) has led to an increased awareness of the potential threat of these viruses. With the discovery of these new human coronaviruses, it has become clear that the potential for another outbreak by a yet unknown human coronavirus is a very …
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Coronavirus Replicase Proteins: Multifunctional Mediators of Replication and Innate Immunity Evasion
… replication complex. In addition, nsp3 from MHV, HCoV-NL63, and SARS-CoV has been shown to possess deubiquitinating activity, which negatively influences the induction of type I IFNs. Consistently, the PLP domains were shown to inhibit the induction of IFN-β-, IRF-3- and …
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Human coronavirus NL63 envelope protein-protein interactions: role in pathogenesis
Human coronaviruses (HCoVs) have garnered intense interest due to the large outbreaks caused by severe acute respiratory syndrome coronavirus 1 (SARS-CoV-1), Middle East respiratory syndrome coronavirus (MERS-CoV), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which have …
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Antibody production with synthetic peptides against human Coronavirus-NL63 ORF3 protein
Human Coronavirus (HCoV) NL63 was detected in an infant in the Netherlands in 2004 and will hereafter be referred to as CoV-NL63. Four years later, it was classified into Group 1 coronaviruses by the International Committee for Taxonomy of Viruses (ICTV), which was modified into alphacoronavirus in …
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A reverse genetics study of the Human Coronavirus 229E ADP-ribose-1"-phosphatase
… associated with Human coronavirus 229E (HCoV-229E) nsp3 were characterised. <br/><br/>First, N-terminal processing of HCoV-229E nsp3 by viral papain-like proteases (PLP) 1 and 2 was investigated. The two proteases were previously shown to have partially overlapping substrate specificities …
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Regulation of IRF-3-Dependent Innate Immune Signaling Pathway by the PLpro Domain of Non-Structural Protein 3 (NSP3) of Severe Acute Respiratory Syndrome (SARS) Coronavirus
… for stable expression of the full length nsp3 of HCoV-OC43, HCoV-NL63, MERS-CoV, and SARS-CoV, respectively. Although these cell lines exhibited stable and tight control of nsp3 expression in the presence of tetracycline, I observed a variation in CoV’s nsp3 protein expression levels. However, …