Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"HCT116 cells"”.
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Influence of Human Papillomavirus 16 E6 on TRAIL-Mediated Apoptosis
… -independent pathways in two cellular models: HCT116 colon carcinoma cells and U2OS osteosarcoma cells. We show that transfection of the HPV 16 E6 oncogene into HCT116 cells provides protection from TRAIL-mediated apoptosis. Additionally, we demonstrate that the protection provided by E6 is …
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Effects of m6A DNA methylation by bacterial methyltransferase in colorectal cancer
… (TME) and within colorectal cancer (CRC) cells. This clade DNA methylation pattern is primarily catalyzed by a cell-cycle regulated methyltransferase (CcrM) ortholog, M.FnI, that targets the GANTC sequence motif through methyl-6-Adenine (m6A) DNA methylation. We hypothesized that M.FnI …
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Biochemical and Molecular Characterisation of the FADDosome
… However, acquisition of resistance by cancer cells is the main cause of the limited clinical use of 5-FU, which needs to be overcome to widen and lengthen its utility. Therefore, it is necessary to investigate the factors and pathways involved in 5-FU-induced apoptosis. Chemotherapeutic …
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Development of advanced 3D tissue models and O2 imaging methodologies
… oxygenation following seeding with both PC12 and HCT116 cells. Using TCSPC and PLIM multiplexed with fluorescent markers and fluorescent staining we could non-invasively correlate lifetime values with toxicity and drug stimulation. Several novel O2 and temperature-sensitive nanoparticle probes …
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Identifying Novel Interaction Between the E3 Ubiquitin Ligase Ring Finger Protein 20 (RNF20) and the Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
… comparing parental and USP7 knockout HCT116 cells, reconstitution assay, overexpression with USP7 or the catalytic mutant USP7, cycloheximide chase and a deubiquitination assay, we show that RNF20 stability is dependent upon USP7 removing ubiquitin chains that would mark RNF20 for …
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A NOVEL PRO-OXIDANT ROLE OF GELSOLIN IN CANCER CELL INVASION
… cell invasion. Increased gelsolin expression in HCT116 results in heightened ROS levels, particularly the superoxides (O2-) as well as increased invasion; whereas silencing of gelsolin reduces O2- levels and invasion. Inhbition of O2- produciton using diphenyleneiodonium significanlty attenuates …
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Nanosecond Pulsed Electric Field Effects on Cell Cycle and Apoptosis
… and maintenance of tissue homeostasis where cells induce their own cell death. Cells undergoing apoptosis exhibit a distinctive phenotype characterized by maintenance of membrane integrity, cell shrinkage, phosphatidylserine (PS) externalization at the plasma membrane, caspase protease …
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Analysis of a novel cytotoxic drug induced TRAIL-R2 signalling complex using a new FACS-based Smac/DIABLO release assay
… of programmed cell death (apoptosis) in tumour cells is an essential cellular and molecular process in response to cancer treatment and important for its success. Classically two forms of apoptosis have been distinguished, the extrinsic and the intrinsic apoptosis. Recently novel mechanisms have …
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Synergic Effect on Cell Death by Co-treatment with TRAIL and Human Anti-DR5 Antibody
… induces apoptotic cell death in cancer cells but not in normal cells. In previous study we isolated a human single-chain variable fragment (scFv) antibody (HW1) that recognizes a different epitope on DR5 form that of TRAIL and induced autophagic cell death. when treated to various cancer …
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Genetic Approach to Discover ARMC4 as a Novel NF-κB Negative Regulator and Tumor Suppressor in Colorectal Cancer
… control group, overexpression of ARMC4 in HEK293 cells or CRC HT29, DLD1, and HCT116 cells dramatically reduced NF-κB activity, cellular proliferation, anchorage-independent growth, and migratory ability in vitro, and unsurprisingly, significantly decreased xenograft tumor growth in vivo. In …
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Real-Time Bioluminescence Imaging Of Salmonella-Neoplastic Cell Interactions
… and replicate in host intestinal epithelial cells and macrophages. Interestingly, Salmonella is also capable of localizing to tumors in in vivo model systems, and while the typical route of Salmonella infection and pathogenesis has been thoroughly investigated, the behavior of Salmonella in …
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ELUCIDATION OF MECHANISMS GENERATING 5-HYDROXYMETHYLCYTOSINE (5hmC) IN MAMMALIAN MITOCHONDRIA
… and percoll purified mitochondrial fractions of HCT116 cells. Mitoprot analysis on Tet family predicts that Tet1 may be translocated to the mitochondria. Immunoblot analysis indicates that a band of expected size(235kDa) is present on immunoblots of mitochondrial fraction from mouse embryonic …
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Role of lncRNA in cancer development and progression
… to -1783bp. In breast and colorectal cancer cells and tissues, PICART1 expression was decreased. Ectopic expression of the PICART1 suppressed growth, proliferation, migration, and invasion of MCF7, MDA-MB-231 and HCT116 cells whereas silencing of PICART1 stimulated the cell growth and …
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Targeting the intestinal stem cell niche through energy and metabolism signaling, for colorectal cancer chemoprevention using aspirin and metformin
… ‘stemness’: the ability of intestinal stem cells to self-renew or differentiate. The mechanistic target of rapamycin (mTOR) pathway, a central metabolic regulator, is elevated in CRC and modulates stem cell response to nutrients. Therefore, chemopreventive agents which regulate nutrient …
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Using synthetic biology to investigate human gene body DNA methylation
… at endogenous loci, I analysed data from HCT116 cells which had been treated with the DNMT1 inhibitor. I found that most genes were unaffected by loss of gene body methylation, although subsets were up- or down-regulated. This suggested that gene body methylation does not exert uniform …
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Targeted Drug Delivery with Cyclodextrin-based Nanocarriers and Focused Ultrasound Triggering
… in vitro human cancer cell modeling with KB and HCT116 cells. A high-throughput in vitro FUS device was designed and applied, in combination with carrier-DOX inclusion. SonoVue® MBs was used to investigate TDD in cell monolayers. Ex vivo and in vivo trials were carried out with a clinically …
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Investigating the role of NEIL3 in colorectal cancer resistance to oxaliplatin
… oxaliplatin have been reported, normal healthy cells also rely on the majority of these mechanisms for survival and genome stability. Recently, NEIL3, a cell-cycle dependent and tissue-specific DNA glycosylase has been implicated in the repair of psoralen- and AP (apurinic) site-induced …
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Elucidating the role of Gremlin1 in colorectal cancer
… and uptake by surrounding colonic epithelial cells. We extended these findings to show that non-GREM1 expressing cells were capable of taking up GREM1 protein via both clathrin- and calveolar-mediated endocytosis. No specific localisation of GREM1 to distinct organelles was identified, but …
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Development of versatile drug delivery strategies using PLGA nanoparticles
… strategies to deliver various drugs to targeted cells were examined.<br/><br/>A NP formulation for the intracellular delivery of plasmid DNA was developed, whereby the DNA was encapsulated within PLGA NP by combining salting out and emulsion-evaporation processes. This process reduced the …
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Inhibition of PLK1 by p53 and by PLK1-targeted drugs
… early phases of the cell cycle and increase as cells go through the cycle, peaking in G2/M. In this thesis, our focus has been on two critical areas in PLK1 research.<br/><br/>1. Tumour suppressor p53 mediates transcriptional repression of PLK1. There have been several mechanisms suggested for …
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