Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 64 for “"HCT116"”.
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Influence of Human Papillomavirus 16 E6 on TRAIL-Mediated Apoptosis
… -independent pathways in two cellular models: HCT116 colon carcinoma cells and U2OS osteosarcoma cells. We show that transfection of the HPV 16 E6 oncogene into HCT116 cells provides protection from TRAIL-mediated apoptosis. Additionally, we demonstrate that the protection provided by E6 is …
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Effects of m6A DNA methylation by bacterial methyltransferase in colorectal cancer
… hypothesis by ectopically expressing M.FnI in HCT116 colorectal cancer cells using expression constructs that encode either the wildtype protein (M.FnIWT) or catalytically inactive mutants (M.FnID33N and M.FnIG219R). Although considerable efforts were spent on optimizing experimental …
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A NOVEL PRO-OXIDANT ROLE OF GELSOLIN IN CANCER CELL INVASION
… cell invasion. Increased gelsolin expression in HCT116 results in heightened ROS levels, particularly the superoxides (O2-) as well as increased invasion; whereas silencing of gelsolin reduces O2- levels and invasion. Inhbition of O2- produciton using diphenyleneiodonium significanlty attenuates …
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TCF4-mediated Transcriptional Regulation of Krüppel-like Factor 4 Gene
… TCF4에 의해서 중재되는지 알아보기 위해, Western blot 실험을 통해 HCT116 세포와 HEK293 세포에서 TCF4 발현을 관찰하였다. TCF4 단백질이 HCT116 세포에서는 발현 되었지만 HEK293 세포에서는 나타나지 않았다. 인간 KLF4 promoter에 존재하는 두 개의 conserved 지역에 TCF4 단백질이 binding 하는지 알아보기 위해, electrophoretic mobility shift assay를 수행하였다. ATCAAAG 지역에는 TCF4 binding complex …
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Dynamic Shuttling of AGO2 to the Nucleus Relieves MicroRNA Repression
… (1) 2D colorectal cancer cell, HCT116, cultures grown beyond a monolayer to high cell density, (2) 3D HCT116 tumor spheroid cultures grown in collagen matrix, and (3) primary tissue samples from malignant colon tumor and normal adjacent colon tissue, show significant nuclear …
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Analysis of a novel cytotoxic drug induced TRAIL-R2 signalling complex using a new FACS-based Smac/DIABLO release assay
… release assay in 5-Flurouracil (5-FU) stimulated HCT116 cancer cells. Using this assay we found that DR5 knock-down cells show a markedly reduced Smac/DIABLO release, which corresponds to decreased mitochondrialmembrane depolarisation in these cells. These mechanistic differences can explain the …
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Synergic Effect on Cell Death by Co-treatment with TRAIL and Human Anti-DR5 Antibody
… cell death type, and signaling molecules in HCT116 cells treated with TRAIL, HW1 and combination of them. Our results showed that the co-treatment of TRAIL and HW1 significantly enhanced cytotoxic effect on HCT116 compared with each single treatment. Transmission electron microscopy analysis …
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Suppression and activation of lipid signalling in cancer : tumour suppressor PTEN and oncogenic sphingosine kinase
… glioblastoma cells, and WT PTEN expressing (A) HCT116 colon cancer and (C) MCF7 breast cancer cells. Transfected cells were then assayed for cell proliferation, cell cycle phase distribution and AKT phosphorylation. In U87MG cells, 50% of the PTEN mutants (Y65C, K125E, E150Q and D153Y) exhibited …
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IGPR-1 promotes colorectal cancer tumor cell survival and modifies the response of cancer cells to chemotherapeutics
… to doxorubicin. Human CRC tumor cell lines, HCT116 and HT29, were transduced via a retroviral system to express IGPR-1 or empty retroviral vector pQCXIP. The effect of overexpression of IGPR-1 in HCT116 and HT29 cells was measured by MTT assay in non-adherent 24-well plates. In addition, …
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Aldehyde dehydrogenases (ALDH) expression in cancer tissues as potential pharmacological targets for therapeutic intervention. Probing ALDH expression and function in 2D- and 3D-cultured cancer cell lines
… CRC cell lines (HT29, DLD-1, SW480 and HCT116) were grown under normoxic or hypoxic conditions (0.1% O2) and HT29 and DLD-1 in spinner flasks to generate multicellular spheroids (MCS). Hypoxia was demonstrated to have an impact on the ALDH expression, which appeared cell-specific. …
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Die funktionelle Bedeutung des Coxsackie- und Adenovirus Rezeptors (CAR) im kolorektalen Karzinom
… In den Zelllinien CaCo2, Colo205, DLD1, HCT116, HT29, SW480 und T84 konnte die Expression von CAR (mRNA und Protein) nachgewiesen werden. Nach stabiler CAR-Überexpression durch Transfektion von CARcDNA in DLD1, HCT116 und SW480 wurde das Zellwachstum gehemmt und eine Abnahme von Migration …
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Aldehyde dehydrogenases (ALDH) expression in cancer tissues as potential pharmacological targets for therapeutic intervention. Probing ALDH expression and function in 2D- and 3D-cultured cancer cell lines
… CRC cell lines (HT29, DLD-1, SW480 and HCT116) were grown under normoxic or hypoxic conditions (0.1% O2) and HT29 and DLD-1 in spinner flasks to generate multicellular spheroids (MCS). Hypoxia was demonstrated to have an impact on the ALDH expression, which appeared cell-specific. …
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Aldehyde dehydrogenases (ALDH) expression in cancer tissues as potential pharmacological targets for therapeutic intervention. Probing ALDH expression and function in 2D- and 3D-cultured cancer cell lines
… CRC cell lines (HT29, DLD-1, SW480 and HCT116) were grown under normoxic or hypoxic conditions (0.1% O2) and HT29 and DLD-1 in spinner flasks to generate multicellular spheroids (MCS). Hypoxia was demonstrated to have an impact on the ALDH expression, which appeared cell-specific. …
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Real-Time Bioluminescence Imaging Of Salmonella-Neoplastic Cell Interactions
… induce pro-inflammatory responses in HCT116 colon carcinoma cells, specifically, NF-κB activation. Then, I performed a high-throughput siRNA screen to identify novel host kinases and phosphatases involved in detection of Salmonellaand activation of NF-κB signaling. For this work, I …
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Biochemical and Molecular Characterisation of the FADDosome
… and Chk2 kinases are activated in 5-FU-treated HCT116 cells and play a key role in the 5-FU-induced DNA damage signal transduction towards caspase-10 upregulation and FADDosome formation. It was found that activation of Chk1 and Chk2 as well as induction of p53 are regulated in an ATR-dependent …
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Development of advanced 3D tissue models and O2 imaging methodologies
… oxygenation following seeding with both PC12 and HCT116 cells. Using TCSPC and PLIM multiplexed with fluorescent markers and fluorescent staining we could non-invasively correlate lifetime values with toxicity and drug stimulation. Several novel O2 and temperature-sensitive nanoparticle probes …
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Role of ß-catenin and Leukotriene in Colon Cancer Progression
… to the nucleus which induce proliferation of HCT116 colon cancer cells through phosphorylation of GSK-3β and activation of Tcf/Lef. Furthermore, LTD4 decrease both membranous E-cadherin and β–catenin which lead to an increase migration of HCT116 colon cancer. I also found that membrane …
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Targeting the intestinal stem cell niche through energy and metabolism signaling, for colorectal cancer chemoprevention using aspirin and metformin
… metformin also reduced stem cell function in the HCT116 cell line. Drug combination showed a stronger effect compared to either drug alone, and synergy assays confirmed that drug combination has a synergistic effect. These effects on the stem cell niche were further validated in mouse Apcfl/fl …
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“Priming” as a novel strategy to enhance chemotherapeutic efficacy
… of metabolism were assayed in 3 cell lines HCT116 (Human colon cancer), MCF7 (Human breast cancer) and the control cell line MCF10 (non-cancerous human breast) following 24-hour acetate treatment in doses ranging from 1-25 mM. Reactive oxygen species and Ca2+ were measured with fluorescent …
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Identifying Novel Interaction Between the E3 Ubiquitin Ligase Ring Finger Protein 20 (RNF20) and the Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
… comparing parental and USP7 knockout HCT116 cells, reconstitution assay, overexpression with USP7 or the catalytic mutant USP7, cycloheximide chase and a deubiquitination assay, we show that RNF20 stability is dependent upon USP7 removing ubiquitin chains that would mark RNF20 for …
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