Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"HCT-8"”.
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Evaluation of hops essential oil cytotoxicity and anti-cryptosporidium activity
… vitro cytotoxicity (IC50) were determined in an HCT-8 intestinal cell model using flow cytometry and staining for non-viable cells with propidium iodide after treatment with controls and different concentrations of bioactives of interest (i.e. HEO and myrcene) for 24 and 48 h (37℃ and 5% CO2). To …
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Novel Design Strategy for Potent and Selective CDPK1 Inhibitors for Cryptosporidiosis
… good Cp growth inhibition (IC50 = 0.04 μM) in HCT-8 host cells and no acute toxicity in mice. However, UH15-16 also moderately inhibited human Src kinase (IC50 = 0.28 μM), which raises a toxicity concern. Thus, enhancement of kinase selectivity was needed. Herein, we demonstrated that targeting …
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Studies on the novel detection and destruction of the enteroparasite Giardia lamblia and other veterinary problematic microorganisms examined under varying culture conditions
… exists in Ireland by using a combined in vitro HCT-8 cell culture-quantitative PCR assay for evaluating the efficacy of using pulsed UV light for treating G. lamblia parasites. This study aims to provide a source of data into novel disinfection mechanisms such as Pulsed UV light technologies as …
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Lifecycle Progression And The Sexual Development Of The Apicomplexan Parasite Cryptosporidium Parvum
… infection in cancerous cell lines (HCT-8 and Caco-2) lasts for only three days but mice stay infected for a month We engineered a strain that allows to discern different stages and used it to study the developmental kinetics in HCT-8 cells and mice. Parasites replicated asexually in …
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Evaluation of chemical treatments and ozone on the viability of Cryptosporidium parvum oocysts in fruit juices
… assay, using a human illeocecal cell line (HCT-8) that is most similar to human infection. The percent inhibition of infection by each treatment was determined along with the corresponding log reduction for the treatments found to be most effective. Infection by treated oocysts was compared …
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Effect of Metabolic Enzymes on Amylopectin Content and Infectivity of Cryptosporidium parvum
… react with amylopectin followed by incubation on HCT-8 cells for 24 hours for infection. Real-time PCR and immunohistochemistry were performed to determine the effect of the enzymes on infectivity. An amylopectin assay and excystation assay was performed to determine if the enzymes degraded …
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Lipid-based nutrient supplement to address child undernutrition and enteric parasitic infections in India
… of parasite C. parvum invasion and infection of HCT-8 cells. Finally, β-Cyclodextrin (β-CyD) encapsulation of OEO and carvacrol (1:1 molar) was utilized to mask the potent flavor of bioactives, and achieve controlled intestinal delivery of bioactives, measured using triangle sensory test and …
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Cancer metastasis and elasticity of microenvironment
… concern in colon cancer. We discovered that HCT-8 colon cancer cells can be induced to undergo a phenotypic transition similar to the early stage of metastasis (MLP, i.e. metastasis-like phenotype) simply by growing them on a substrate with appropriate mechanical stiffness (Chapter 2). This …