Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"HCT-116"”.
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The effect of RAN inhibition on human colorectal cancer cells (CRC)
… human cell lines with specific mutations (HCT-116 K-Ras, DLD-1 K-Ras and Pten deletion and wild type HCT-116 and DKO-3. I used qRT-PCR and western blotting to identify expression levels of various genes and signalling molecules after treatment with 0.5 mM MBZ. In addition, several assays …
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Synthesis and bioactivities of novel N¹-acylhydrazides
… the auxiliary binders 60m (IC50 = 15.27 µM in HCT 116 cells) and 60l (IC50 = 3.18 µM in MCF-7 cells) were the most potent cytotoxins in series 60. The evaluation of druglike properties revealed that 64a is the lead tumor-specific cytotoxin with an IC50 value of 1.38 µM against HCT 116 cells. …
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Analysis of the involvement of exosomal miRNAs and proteins in the response of CRC cells to Cetuximab
… le Caco-2 (aventi il gene KRAS normale) e le HCT-116 (con il gene KRAS mutato). Per effettuare tale studio, è stata utilizzata la tecnologia della Real-Time PCR che ha consentito di verificare l’espressione di 745 microRNAs. I risultati hanno mostrato che il profilo di espressione dei …
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PROTEOMICS INSIGHTS INTO MOLECULAR MECHANISMS OF TRIPTOLIDE AGAINST COLORECTAL CANCER
… factors in colorectal cancer cell line HCT 116. In my first project, protein alterations of HCT 116 cells treated with triptolide were profiled at a global level, by using Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) labeling followed by MS/MS analysis. The study of …
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Inhibitory potential of anthocyanins and anthocyanin-rich extracts on colorectal cancer progression through apoptosis, angiogenesis, and immune response effects
… Throughout, we used two colon cancer cell lines, HCT 116 and HT-29, which have different phenotypic and genotypic characteristics to give a more thorough analysis of the anthocyanins and anthocyanin-rich extract effects. The first aim utilized colored corn extracts and showed anti-proliferative …
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Metabolic Manipulation of Glutathione as an Anticancer Therapeutic Target
… a putative inhibitor of the thioredoxin system. HCT 116 p53-/- cells were resistant to auranofin at doses that HCT 116 p53+/+ cells were sensitive to, however concurrent exposure of auranofin and glutathione depletion through serine and glycine starvation sensitized HCT 116 p53-/- cells to …
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Effects of Green Tea Extract (GTE) for Normal and Cancer Cell Death by Radiation
… 하였다. 본 실험에서는 마우스의 정상 소장 상피세포주인 IEC-6과 인간 대장암세포주인 HCT-116을 사용하였다. 방사선조사는 6MV 선형가속기를 이용하였고 실온에서 0, 2, 4, 6 Gy의 선량으로 조사하였다. 다양한 농도의 녹차추출물을 단독으로 처리하거나 녹차추출물 처리 후 방사선을 조사하고 MTT assay, clonogenic assay, ROS assay를 시행하였다. 방사선과 함께 0.025 및 0.05 mM의 녹차추출물을 사용하였을 때 방사선만을 조사받은 경우에 비해 정상 세포주인 IEC-6에서 방사선조사 후 …
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Novel Cellular Targets of Aspirin in Chemoprevention studies on P53, G6PD and c-MYC
… these proteins in MDA-MB-231 human breast and HCT 116 human colon cancer cells, leading to modulation of their functions. It was therefore important to extend these studies to other cancer cell lines to gain an insight into the mechanisms of chemoprevention by aspirin, and to more clearly …
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LncRNA and circRNA expression profiles in tissues and serum exosomes of colorectal cancer patients and cell lines
… expression of HOTAIR, MALAT1, TUG1 and UCA1 in HCT-116 colon cancer cells treated with U0126 (MEK1/2 specific inhibitor) compared to untreated cells, suggesting a possible connection between ncRNAs and MAPKs. Also, we performed a computational analysis to identify mRNAs and miRNAs involved in …
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Komplexkonjugate bioaktiver Flavonoide & Naphthochinone und Photoaktivierung von Carboplatin für Studien der intrazellulären Verteilung
… Komplex 119 eine nennenswerte Aktivität an der HCT-116-Zelllinie mit IC50-Werten im Bereich von 8,7 – 12,1 µM. Als weiterer Flavonoidligand sollte Wogonin (17) dienen. Während der Darstellung von 17 konnte die von Huang et al. publizierte Synthese widerlegt werden.27 Es konnte nur Negletein (71) …
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Identification and bioactivity assessment of a protease inhibitor QUB-1833 from the skin secretion of Lithobates catesbeiana
… and two human cancer cell lines (U251-MG and HCT-116). This peptide has a very low hemolysis activity as well.<br/><br/>In the future, more in-depth research should be based on its good enzyme inhibitory effect, and improves its other bioactivities, so that it can be applied in practice.
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Bioengineered Submucosa for Studying Effects of Microenvironmental Mechanics on Colorectal Cancer Cells
… with varying matrix alignment and embed an HCT-116, colon carcinoma cell line, spheroid into our organoids to simulate an integrated tumor compartment. Through the modulation of our submucosal model, we determine the effects of varying alignment and stiffness on colorectal cancer cell …
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Identification and functional evaluation of a bioactive peptide QUB-1369 Secreted from the skin of The Common Frog (Rana Temporaria)
… µM. Anticancer assay found a weak inhibition of HCT 116 Human Colon Carcinoma cells at a concentration of 100 µM.
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세포자멸사 촉진분자를 이용한 암 치료용 단백질치료제의 개발
… 단백질의 세포자멸사 촉진 효과 분석 - 19 G. Jurkat, JR-T2, HCT-116, HT1080 세포주에서의 endogenous Bax, Bcl-xL, p53 단백질 발현 양상 분석 - 20 H. wild type Bcl-xL(WT)과 돌연변이 Bcl-xL(5A)의 세포 내 위치 분석 - 22 I. wild type Bcl-xL(WT)과 돌연변이 Bcl-xL(5A,5S)의 homodimer 형성 확인 - 22 J. wild type Bcl-xL(WT)과 돌연변이 Bcl-xL(5A)의 Bax와 …
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Regulation of apoptosis in human cancer cells
… human colorectal cancer cells, HT-29 and HCT- 116, and human prostate cancer cells, LNCaP, with cytokines in order to induce cell death. Then, we observed the effects of NO inhibitors, kinase inhibitors, and activation of Akt, a kinase up-stream of the caspase cascade, following …
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Estudio de los lncRNAs MDL1 y MDL1AS en diferentes tipos de tumores y su relevancia clínica en adenocarcinoma rectal
… colorectal cancer cell lines (HT-29 and HCT-116) and breast cancer cell lines (MCF-7 and MDA-MB-231), as well as different double-stranded interference RNAs (DsiRNAs) to knockdown MDL1AS expression and evaluate how this affects cell metabolism, proliferation and migration in the different …
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Development of monoclonal antibodies for the identification of novel invasion associated targets in human cancer.
… cell lines; the SNB-19 glioma cell line and the HCT-116 colon cancer cell line. Inhibition of invasion was also observed in the Lox IMVI melanoma cell line, but not significantly so. This MAb also significantly decreased cell motility in the MiaPaCa-2 clone 3 cell line. MAb 9E1 24 (6) …
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Identification and functional study of a bioactive peptide QUB-2413 from the skin secretion of the Red-Eyed Tree Frog, Agalychnis callidryas
… anticancer activities against U251-MG cells and HCT-116 cells in a range of concentration from 10 -6 M to 10 -4 M. With regard to haemolysis assay, QUB-2413 had no more than 10% haemolysis activity at 512 µM. Although the biological activities of QUB-2413 are not ideal, it is still expected to …
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Development of monoclonal antibody cell culture processes and antibody characterisation
… cell-based assays were explored using HL-60 and HCT-116 cell lines to study IL-8 signalling and anti-IL-8 mAb activity. These assays generated variable and inconclusive results that highlighted the need for further work using alternative mechanism-specific functional assays. Overall, while …
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Effetti antiproliferativi dei surnatanti di Lactobacillus Rhamnosus GG su cellule tumorali
… cellulari di carcinoma del colon umano (HT29, HCT-116, Caco-2) e cellule di melanoma umano (A375)con surnatante di Lactobacillus rhamnosus GG ha mostrato un significativo effetto antiproliferativo dipendente dalla concentrazione, determinato con saggi di vitalità cellulare . In particolare, il …
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