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Showing 1 to 20 of 31 for “"H9C2"”.
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The Cardioprotective effect of a Lanosteryl Triterpene from Protorhus Longifolia on H9c2 Cardiomyoblasts
… stress, insulin resistance and apoptosis in H9c2 cardiomyoblasts exposed to high glucose. Methodology: H9c2 cardiomyoblasts were cultured in either normal (NG- 5.5 mM) or high (HG- 33 mM) glucose for 24 hrs. Subsequently, cells exposed to HG were treated with RA3 (1 μM), n-acetyl cysteine …
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Up-regulation of junctophilin-2 prevents ER stress and apoptosis in hypoxia/reoxygenation-stimulated H9c2 cells
Ischemic heart disease is the leading cause of death, and reperfusion which can restore blood flow is the primary therapy. However, reperfusion can induce further damage to cardiomyocytes, a condition described as ischemia-reperfusion (I/R) injury. I/R is now recognized as a combination determining …
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Stimulated Emission Depletion (STED) Microscopy and Pacific Orange Dye Optimization For H9C2 Cox-1 Imaging Via Indirect Immunocytochemistry
… tests yielded testing parameters for imaging. H9c2 rat embryonic myocardium heart cell samples were passaged and stained via indirect immunofluorescence: COX-1 proteins of the mitochondrial inner-membranes were targeted by primary mouse, anti-COX-1 IgG antibodies, which in turn were targeted by …
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Biochemical effects of organophosphorous compounds on cultured rat and human cardiomyocyte-like cells
… (diazoxon and chlorpyrifos oxon) on rat H9c2 and human cardiomyocyte-like cells. The rat embryonic H9c2 myoblast cell line, which has the ability to differentiate into a cardiac muscle phenotype, can be instrumental in understanding OP cytotoxicity at different differentiation stages. …
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The cardioprotective mechanisms of dietary flavonoids
Mitotic rat embryonic cardiomyoblast-derived H9c2 cells are widely used as a model cardiomyocyte to study the protective mechanisms of dietary flavonoids, but they lack features of fully differentiated cardiomyocytes. Therefore, this present study aimed to investigate the cytoprotective and …
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The effect of Aspalathin on Doxorubicin-induced Cardiotoxicity
… cardiotoxicity in an in vitro H9c2 cardio myoblast cell culture model. Methods: H9c2 cardiomyocytes and Caov-3 cancer cells were cultured in Dulbecco’s modified eagle’s medium and treated with or without Doxorubicin for five days. Thereafter, cells exposed to 0.2 μM Doxorubicin …
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Investigating the protective role of the natural hormone Melatonin, in reducing drug-induced cardiotoxicity in the therapy of chronic diseases
… the role of melatonin in cardioprotection in H9c2, a model rat cell line in vitro. Using the Seahorse XF analyser method, it was demonstrated that commonly used medication for chronic diseases such as amiodarone, amitriptyline, and statins all caused altered mitochondrial dysfunction. In …
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The role of the large conductance Ca2+-activated K+ channel in adenosine receptor-mediated cytoprotection
The rat embryonic cardiomyoblast-derived H9c2 cell line is increasingly used for studies into cardioprotection, as these cells display similar properties to primary cardiomyocytes. Adenosine receptors are well known mediators of cardioprotection and trigger effectors such as the mitochondrial KATP …
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Ethanolamine as a cardioprotective agent in doxorubicin-induced cardiotoxicity
… 10 μM)-induced cytotoxicity were determined in H9c2 cardiomyoblasts, isolated murine cardiomyocytes and H9c2/3T3 coculture. Metabolic activity and cell viability were determined using MTT and LDH assays for H9c2, fibroblasts and coculture experiments, and trypan blue exclusion and LDH assays in …
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Investigating the possible cytoprotective effects of melatonin isomer against simulated ischemic injury
… of melatonin receptors in this effect. Methods: H9C2 cardiac fibroblast cells were subjected to simulated ischemia by exposure to 1mM H₂O₂ following a 30min pre-treatment with 75ng/L (dietary concentration), 1μM (pharmacological concentration, 0.232mg/L) melatonin or/and 75mg/L (dietary …
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Investigations into the influence and role of tissue factor in the pathogenesis of myocardial hypertrophy
… to counteract the effects of TF.Stimulation of H9c2 cardiomyocytes with exogenous TF resulted in the up-regulation of mechano growth factor. Incubation of the cells with TF alone up-regulated atrial natriuretic factor (ANF) expression, whilst the presence of the TF-associated proteases, factor …
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Design and Evaluation of Cyclic Peptide-Epirubicin Conjugate for Enhanced Anticancer Activity and Reduced Myocardial Toxicity
… (MES-SA/MX2), and normal rat cardiomyoblast (H9C2) cells. The conjugate (5 µM) exhibited significantly higher antiproliferative activity against breast cancer cells and DOX/EPI-resistant cells and less antiproliferative activity against SK-OV-3 cells, while it showed minimal cytotoxicity …
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Cardioprotective Effect of Onion Extract against Ischemic Injury In vitro and In vivo
… effect against ischemic injury in H9c2 cell culture system and rat myocardial infarct model in vivo, and if so, whether the beneficial effect of onion is attributed to its antioxidant property. This study also investigated which component of onion extract is involved in the …
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The Effects of Cocaine and Ecstasy on Cardiac Myocytes and the Intact Myocardium
… heart function. Cultured cardiac myocytes (H9c2) and New Zealand White rabbits (Oryctolagus cuniculus) were used to measure the responses to various concentrations of cocaine or ecstasy at both the cellular and intact organ system levels. We observed that cocaine and ecstasy significantly …
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THE BIO-MOLECULAR ANALYSIS OF TITANIUM DIOXIDE NANOPARTICLES EXPOSURE ON CARDIOMYOCYTES
… identify these biological endpoints. Using the H9c2 cardiomyocyte as an <i>in vitro</i> model, titanium dioxide (TiO₂) NPs was applied to understand how TiO₂ NPs can affect the heart. The study identified the localization of TiO₂ NPs in the endoplasmic reticulum (ER). ER stress and elevated …
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Exploring the in vitro effects of Helichrysum petiolares for its potential to provide cardioprotective benefits against Doxorubicin toxicity
… cytotoxicity in breast cancer cells. Methods: H9C2 cardiomyocytes, MCF-12A non-cancerous breast epithelial cells, and MDA-MB-231 TNBC cells were exposed to 12.5 μg/mL H. petiolare methanol extract or Dox for three hours, followed by inverse or combination treatments for 24 hours under standard …
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Exploring the relationship between shifts in high-density lipoprotein (HDL) particle subclass distribution/functionality and cardiac function in doxorubicin (DOX)-induced cardiotoxicity
… against DOX-induced cytotoxicity was assessed in H9C2 cells and measured using the adenosine-triphosphate (ATP) assay. Results: DOX-induced subclinical cardiotoxicity was observed in 9 breast cancer patients. DOX therapy reduced the intermediate HDL subclasses [52.5±1.0% (B) vs 48.6±0.9% (E), …
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Trasduzione del segnale e poliamine nell'apoptosi di cellule cardiache
… and following treatment with NE or ALD. Methods: H9c2 cardiomyoblasts were exposed to a condition of simulated ischemia, consisting of hypoxia plus serum deprivation. Cardiomyocyte cultures were prepared from 1-3 day-old neonatal Wistar rat hearts. Polyamine depletion was obtained by culturing the …
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Effect of TIS21 on DNA Damage Repair Induced by Etoposide
… type p53 harboring cells such as MCF7, HeLa, and H9C2 cell lines were also confirmed, and it showed same phenomenon with Huh7 cells. Bcl-2-associated X protein (BAX) and p53 upregulated modulator of apoptosis(PUMA), which are known to be pro-apoptotic proteins in p53-dependent apoptosis, were not …
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Flavonoid protection of cardiac cells against ischemia-reperfusion injury
… phase of the studies, rat embryonic ventricular H9c2 cells were treated with various concentrations of polyphenols with or without ascorbate for 1-3 days before induction of ischemia and reperfusion. Ischemia was induced by exposure of the cells to a non-glucose containing solution bubbled with …
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