Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"H3K9me3"”.
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Molecular targets of chromatin marks H3K4ME3, H3K9ME3 and H3K27ME3 in an adult germinal niche
… targets of three histone modifications: H3K4me3, H3K9me3 and H3K27me3. Our data suggest these chromatin marks are associated with genes responsible for cellular organization and morphology, proliferation and survival, neuron development. Taken together these processes suggest histone …
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Functional characterization of CDY family proteins and their role in recognition of the heterochromatic histone H3K9me3 modification
… von dem Lysin 9 in der Sequenz des Histons H3 (H3K9me3). H3K9me3 stellt ein Markenzeichen des Heterochromatins dar, welches dicht gepackt und weitgehend transkriptionell inaktiv ist und während der S-Phase spät repliziert wird. Die vorliegende Doktorarbeit charakterisiert eine Gruppe von …
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Large replisome-dependent heterochromatic domains defining the transgenerational regulatory landscape
… trimethylation in lysine 9 and 27 of histone H3 (H3K9me3 and H3K27me3) are associated with gene repression, whilst euchromatic marks are associated with gene activation. Several components of the DNA replication machinery, such as the helicase subunit MCM-2, the leading strand polymerase Polε …
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Unlocking Compacted Heterochromatin Through Modulation Of Associated Proteins
… gene and repetitive element silencing. As H3K9me3 can block cellular reprogramming, we used a human-induced fibroblast to hepatocyte (hiHep) reprogramming model to uncover diverse genes and repetitive elements silenced by each protein. We conducted RNA-seq experiments both with and without …
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CONSEQUENCES OF CHROMATIN MOSAICISM AND PERSISTENCE IN NEURONAL PLASTICITY AND DISEASE
… In FXS, using imaging and genome-wide H3K9me3 profiling in patient-derived neural progenitor cells, I find that the mutation-length CGG expansion on the X chromosome drives inter-chromosomal clustering of heterochromatin domains at Megabase-scale H3K9me3 domains, termed BREACHes. …
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Molekulare und funktionelle Analyse von Windei (CG12340) als Bindungspartner der Histonmethyltransferase Eggless während der Oogenese von <i>Drosophila</i>
… Die Trimethylierung von Histon 3 am Lysin 9 (H3K9me3) führt zur Ruhigstellung des Chromatins und zur Bildung von Heterochromatin durch die Rekrutierung von Heterochromatin Protein 1 (HP1). Eggless (Egg), das Ortholog der humanen Methyltransferase SETDB1, ist für die H3K9-Trimethylierung in der …
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Quiescence induces epigenetic changes in bovine fibroblasts and improves their reprogramming into cloned embryos
… histone 3 (H3), specifically H3K4me3, H3K9me2, H3K9me3 and H3K27me3, but not H3K9me1. Histone acetylation was reduced at H3K9 and H4K5, increased at H3K12 and remained unchanged at H3K16. G₀ cells also significantly reduced DNAme. In addition, they significantly down-regulated the nuclear …
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Epigenetic repression of intronless mobile elements by the HUSH complex
… through histone H3 Lys9 trimethylation (H3K9me3). How HUSH recognizes and initiates silencing of these invading genetic elements is unknown. By monitoring transcription from L1 transgenes, I found that incoming L1s are recognized by HUSH independently of their integration sites, a …
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UHRF1 and the DNA Damage Response
… mark known as histone 3 lysine 9 trimethylation (H3K9me3). H3K9me3 has been shown to play a role in a signaling pathway involved in the repair of DNA damage in heterochromatic regions. In the fourth chapter of my thesis, we hypothesize that UHRF1 is playing a role in a pathway that responds to DSB …
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Transposable Elements in Neural Progenitor Cells
… cells are repressed by the establishment of H3K9me3-associated heterochromatin. De-repressed MMERVK10C and IAP1 furthermore activate nearby genes and generate long non-coding RNAs. Homozygous TRIM28 knockout is lethal, while mice with mono-allelic TRIM28 expression are characterised with a …
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Determinants of piRNA precursor transcription and export in Drosophila melanogaster
… been shown to bind to the heterochromatic mark H3K9me3 at piRNA clusters, the determinants that specify the exclusive association of RDC with dual-strand clusters remained largely unknown. For the first part of this thesis, I performed a genetic screen in Drosophila germ cells to identify …
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Regulation of cancer progression by hepatitis B virus X protein (HBx) and HBxAP/RSF1
… 39 3. The centromeric expression level of H3K9me3 is downregulated at metaphase in RSF1 knock-down cells 43 4. RSF1 depletion enhances the expression levels of H3K9me3 and H3K27me3 in mitosis 46 5. RSF1 dissociates from histone peptides with phosphorylated H3S28 modification 46 6. RSF1 …
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Mechanisms and functional significance of nuclear compartmentalization
… to the nuclear periphery and confer increased H3K9me3 modification, as assayed both by immunofluorescence and chromatin immunoprecipitation. PTRs within HBB BACs bias a competition between peripheral versus pericentric heterochromatin (PCH) targeting towards peripheral targeting. Targeting to …
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Bioinformatic methods for the analysis of genetic variability and regulation
… with ChIP-seq) libraries of H3K4me3, H3K27Ac, H3K9me3, and H3K27me3 on mouse embryonic stem cells. We started by asking what are the genome coordinates of each nucleosome in the mouse genome. To gain sensitivity, we combined all four MNChIP-seq libraries with publicly available MNase-seq data. …
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INVESTIGATING THE LINK BETWEEN HOMOLOGOUS RECOMBINATION PROTEIN BRCA2 AND DNA METHYLATION
… the histone methylation pathways, particularly H3K9Me3 revealed a strong counter-effect to the protection offered by the loss of DNA methylation, suggesting that chromatin state dictates fork vulnerability to nucleases. Our findings outline a model where cytosine methylation promotes genome …
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Role of WD domain-containing proteins in cell cycle progression
… enriched for heterochromatic marks, including H3K9me3 and methylated-CpGs. ORCA co-localizes with a subset of origins that are enriched for repressive marks, consistent with the fact that these origins replicate late during S phase. Further, ORCA directly associates with the repressive marks …
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Stochastic Activation of Enhancers in the Innate Immune Response by the Histone Demethylase JMJD2D
… the transition of enhancers from 'poised' (H3K9me3) to 'active' (H3K9ac) that allows for eRNA production. In support of this hypothesis, we observe that JMJD2d is tightly associated with enhancers in the genome and preferentially binds active enhancer regions. Taken together, JMJD2d …
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Mechanistic and Epigenetic Partitioning of Lamina-Associated Chromatin Revealed by a Genome-Wide Imaging Screen
… LADs, which are predominantly marked by H3K9me3. Collectively, these findings uncover a pivotal role for hnRNPK in spatial genome organization, highlight the broader diversity of LAD localization mechanisms, and establish a framework for systematically mapping genetic control of nuclear …
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The KRAB Zinc Finger Protein ZKSCAN3 binds to a SINE-embedded regulatory sequence to repress enhancer activity
… of heterochromatin, including SETDB1- mediated H3K9Me3. However, KZFPs are increasingly recognised as being integrated into gene regulatory networks through ‘domesticating’ transposable elements to act as cis-regulatory sequences. Zinc Finger with KRAB and SCAN domains 3 (ZKSCAN3) is a member of …
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Transcriptional and Chromatin Disorientation Associated with BRCA2 Inactivation
… BRCA2 deficient cells had a loss of repressive H3K9me3 mark which was associated with chromatin accessibility. Importantly, this chromatin de-repression was accompanied by increased expression of repetitive RNAs, which have been implicated in inducing DNA damage, genomic instability, and …
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