Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 54 for “"H3K4me3"”.
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Molecular Functions of MLL PHD3 Binding to Its Ligands Cyp33 and H3K4Me3
… that trimethylates histone H3 on lysine 4 (H3K4Me3), a histone modification that correlates with active transcription. The 3rd PHD finger of MLL binds to H3K4me3. Thus MLL is a "writer" with an embedded "reader" for H3K4Me3. Cyp33 is another known ligand of MLL PHD3. Over expression of Cyp33 …
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Molecular targets of chromatin marks H3K4ME3, H3K9ME3 and H3K27ME3 in an adult germinal niche
… gene targets of three histone modifications: H3K4me3, H3K9me3 and H3K27me3. Our data suggest these chromatin marks are associated with genes responsible for cellular organization and morphology, proliferation and survival, neuron development. Taken together these processes suggest histone …
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Investigation of transcriptional regulation by C. elegans SET-2/SETD1 H3K4 methyltransferase through single-nucleus profiling
… these, trimethylation of lysine 4 on histone H3 (H3K4me3), catalyzed by the SETD1 family of histone methyltransferases, is a well-established hallmark of active promoters in eukaryotes. Although loss of SETD1 function results in transcriptional deregulation in many systems, the relationship …
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Molecular and epigenetic mechanisms of fear memory
… The trimethylation of histone 3 lysine 4 (H3K4me3) is an essential regulator of active gene transcription in cells and has been shown to be critical for memory formation in the hippocampus, a major site of memory storage. However, it is unknown how H3K4me3 is coordinated to target genes …
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THE APPLICATION OF HISTONE ANALYSIS FOR IDENTIFYING DISTAL REGULATORY ELEMENTS - REGULATION OF FSHR
… globally map Histone 3 Lysine 4 tri-methylation (H3K4me3) in Sertoli and myoid cells. H3K4me3 is a post-translational histone modification known to associate with distal regulatory elements and promoter regions of actively transcribed genes. Analysis of H3K4me3 enrichment profiles identified a …
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Discovery of Novel Ubiquitin- and Methylation-Dependent Interactions Using Protein Domain Microarrays
… the histone H3 N-terminal tail harboring either H3K4me3 or H3K4me0. Structural studies have identified common features among different H3K4me3 effector PHDs: Cleaved initiator methionine: a groove that fits the R2 residue, and an aromatic cage that engages the K4me3. We hypothesize that some PHDs …
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Epigenetic Regulation of Muscle Stem and Progenitor Cells
… promoter, Mll1 KO results in a minor decrease of H3K4me3 at Pax7, supporting a recognized non-HMT role for Mll1 at Pax7. Microarray analysis of mRNA expression in Mll1 KO myoblasts finds that Myf5 is the most strongly downregulated of all genes, unexpectedly, mRNA expression of previously …
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Dynamic histone modifications at the promoters of Hox genes in embryonic stem cells
… Levels of the “active” modifications H3K9ac and H3K4me3 at Hoxb promoters varied widely from gene to gene, but were closely correlated in ES cells. Contrastingly, the repressive modification H3K27me3 was found at equivalent levels across the cluster. Treatment with the histone deacetylase …
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Stat3 Controls The Neutrophil Migratory Response to Cxcr2 and Its Ligand Mip-2 (Cxcl2)
… levels of activating trimethylated lysine 4 (H3K4me3) modifications relative to repressive trimethylated lysine 27 (H3K27me3) marks, suggesting G-CSF/STAT3 signals stimulate an open chromatin configuration at these promoters. This is further supported by the observation that pharmacologic …
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THE IMPACT OF REPLICATION STRESS ON THE EPIGENOME OF MOUSE EMBRYONIC STEM CELLS
… a global loss of H3K27me3 and the maintenance of H3K4me3 at gene promoters following aphidicolin-induced replication stress. Notably, the activation of a subset of bivalent developmental genes – marked by both H3K27me3 and H3K4me3 – was associated with extra-embryonic differentiation. Furthermore, …
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Role of Histone Lysine Demethylase, KDM1B, in Trophoblast Stem Cell Self-Renewal and Differentiation
… relevant histone modifications H3K4me1, H3K4me2, H3K4me3 and H3K36me3, I show that KDM1B regulates intragenic H3K4me1-marked enhancers, while not impacting H3K4me3. Remarkably, KDM1B null trophoblast cells also show an increased instability of chromosome 13, the same chromosome on which Kdm1b …
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Quiescence induces epigenetic changes in bovine fibroblasts and improves their reprogramming into cloned embryos
… at lysines (K) of histone 3 (H3), specifically H3K4me3, H3K9me2, H3K9me3 and H3K27me3, but not H3K9me1. Histone acetylation was reduced at H3K9 and H4K5, increased at H3K12 and remained unchanged at H3K16. G₀ cells also significantly reduced DNAme. In addition, they significantly down-regulated …
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Roles of the Histone Chaperone FACT in Drosophila Gene Regulation and Chromatin Architecture
… including histone 3 lysine 4 trimethylation (H3K4me3) and histone 3 lysine 36 trimethylation (H3K36me3), in the promoters and transcribed regions of genes. In particular, H3K4me3, a mark of active promoters, was reduced at promoters and increased in gene bodies of the FACT depleted cells. …
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Regulation of Chromatin Landscape on and by the Human Sex Chromosomes
… Xi-expressed and Xi-silent genes, we quantified H3K4me3, H3K27me3, and CTCF along Xi by linear modeling in cells of individuals with zero to three Xis. We demonstrate that Xi-expressed genes are enriched for H3K4me3 compared to Xi-silent genes. Moreover, Xi-silent genes near strongly Xi-expressed …
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Modeling the Heterogeneous Brain Tumor Microenvironment to Analyze Mechanisms of Vascular Development and Chemoresistance
… culture dimensionality and oxygen status. The H3K4me3 modification we analyzed is associated with increased gene transcription, therefore the alterations we observed in H3K4me3 binding across the genome could be a mechanism by which the tumor microenvironment is regulating cancer cell …
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Global alterations of the epigenetic landscape define endometrial receptivity and provide a hallmark for endometriosis
… histone modification profiles by ChIP-seq for H3K4me3, H3K9me3 and H3K27me3, as well as DNA methylation (5mC) and hydroxymethylation (5hmC) profiles by meDIP-seq and hmeDIP-seq, respectively, of endometrial biopsies from control and endometriosis patients. My data reveal considerable changes to …
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Role of Chromatin Structure and JmjC Histone Demethylases in the Response to Hypoxia
… active transcription histone methylation marks, H3K4me3 and H3K36me3, were mapped by ChIP sequencing in the acute response to hypoxia. This analyses found specific changes in histone methylation, which correlate with the core gene transcription changes in hypoxia, pointing towards a mechanism by …
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Characterising the gene regulatory landscape of CD4+ T cells
… (ChM) sequencing protocol to profile H3K4me3 and H3K27ac histone modifications in Tregs. I combined it with chromatin accessibility and gene expression profiling in resting and stimulated Tregs from ten donors. I observed cases of alternative transcription, such as alternative splicing …
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Investigating the role of DPPA2 and DPPA4 in the Epigenetic Control of Lineage Programs in Human Embryonic Stem Cells
… and repressive modifications, such as H3K27me3, H3K4me3 and H3K4me1. Combining opposing modifications may help to maintain robustness of genes to low levels of signal, whilst retaining the capacity to respond upon the appropriate level. Despite their importance in controlling cell fate decisions, …
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Bioinformatic methods for the analysis of genetic variability and regulation
… of DNA coupled with ChIP-seq) libraries of H3K4me3, H3K27Ac, H3K9me3, and H3K27me3 on mouse embryonic stem cells. We started by asking what are the genome coordinates of each nucleosome in the mouse genome. To gain sensitivity, we combined all four MNChIP-seq libraries with publicly …
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