Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"H3K36me3"”.
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The role of the PWWP domain in the DNA methyltransferase 3A targeting to the genome
… with histone 3 lysine 36 trimethylation (H3K36me3). In this study, I characterise a mouse model carrying a D329A point mutation in the DNMT3A PWWP domain, predicted to ablate the DNMT3A-PWWP and H3K36me3 interaction. Similar mutations in humans cause primordial dwarfism. I found that …
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Roles of the Histone Chaperone FACT in Drosophila Gene Regulation and Chromatin Architecture
… and histone 3 lysine 36 trimethylation (H3K36me3), in the promoters and transcribed regions of genes. In particular, H3K4me3, a mark of active promoters, was reduced at promoters and increased in gene bodies of the FACT depleted cells. Furthermore, levels of H3K36me3, a mark of active …
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Role of Histone Lysine Demethylase, KDM1B, in Trophoblast Stem Cell Self-Renewal and Differentiation
… modifications H3K4me1, H3K4me2, H3K4me3 and H3K36me3, I show that KDM1B regulates intragenic H3K4me1-marked enhancers, while not impacting H3K4me3. Remarkably, KDM1B null trophoblast cells also show an increased instability of chromosome 13, the same chromosome on which Kdm1b itself is …
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Molecular Characterization of Mammalian De Novo DNA Methyltransferase Chromatin Recruitment
… gene bodies through PWWP domain interaction with H3K36me3, thereby resembling the genomic localization of DNMT3B. Lastly, loss of PWWP domain reader functionality reveals the presence of a previously uncharacterized chromatin reader domain within DNMT3A that serves as an alternative targeting …
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Epigenetic Regulation of Replication-Dependent Histone mRNA 3 End Processing
… Histon H3 Lysin 4 Trimethylierung (H3K4me3) und H3K36me3 von Bedeutung ist. Allerdings scheinen diese Modifikationen nicht ausschließlich von der CTD-Phosphorylierung an Serin 2 abhängig zu sein. Sie können zudem von der Aktivität von SUPTH5 (suppressor of Ty 5 homolog), NELF-E (negative …
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IDENTIFICATION OF CANCER- AND METASTASIS-RELATED EPIGENETIC DYNAMICS USING PATIENT-DERIVED ORGANOIDS
… five histone marks (H3K27ac, H3K4me1, H3K4me3, H3K36me3 and H3K27me3) through ChromHMM, modelling chromatin into 12 biologically distinct states. We then focused on active enhancers to perform a differential analysis between primary and metastatic CRC PDOs. Next, we annotated the enhancers …
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Role of Chromatin Structure and JmjC Histone Demethylases in the Response to Hypoxia
… histone methylation marks, H3K4me3 and H3K36me3, were mapped by ChIP sequencing in the acute response to hypoxia. This analyses found specific changes in histone methylation, which correlate with the core gene transcription changes in hypoxia, pointing towards a mechanism by which rapid …
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Characterizing interactions between the cellular HIV-1 integration cofactor, LEDGF/p75 with chromatin and HIV-1 integrase
… histone post-translational modification (PTM), H3K36me3, were characterized and significance for high affinity binding monitored. Determining the details of the interaction between the LEDGF/p75 PWWP domain and active gene regions in chromatin could aid in the development of a novel class of …
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Genistein suppresses colon cancer metastasis through epigenetic regulations
… and altered histone structure of the gene. H3K36me3, a positive histone modification, was increased by genistein at 5’untranslated region (5’UTR) and distal downstream regions. Genistein also caused global decrease of H3S10p in both cell lines. The following study aimed at investigating the …