Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"H3.3"”.
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Control of Regulatory Element Function by Histone H3.3
… modifications (PTMs). The histone variant H3.3 is enriched at transcriptionally active regulatory elements such as promoters and enhancers. While recent studies have revealed a role for H3.3 in silencing repetitive elements and repressing developmentally regulated promoters, it is unclear …
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The role of the histone variant H3.3 and its chaperones in the response to DNA damage
H3.3 is a histone variant without a clear, single function. Unlike the canonical replicationassociated H3.1 and H3.2, it is present throughout the cell cycle and can be deposited into chromatin by two distinct and specific chaperone complexes: ATRX/DAXX and HIRA. H3.3 and its chaperones have been …
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Genome-Wide Localization And Novel Deposition Pathways Of Histone Variant H3.3 In Embryonic Stem And Neuronal Precursor Cells
… the first genome-wide profiles of histone H3 variants in pluripotent mammalian embryonic stem (ES) cells, and I establish the dependence and independence of these patterns on the histone chaperone Hira. To distinguish H3 variants, I use designed zinc finger nucleases (ZFNs) to rapidly knock …
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Identifying the Role of DAP-1 and H3.3 Histones in Starvation and Longevity in <i>Caenorhabditis Elegans</i>
… two proteins: HIS-71- a highly conserved histone H3 variant, and DAP-1- which is highly conserved in animals, but nevertheless poorly characterized. We found that HIS-71 is required for mediating increased longevity after intermittent starvation. We started to investigate the role of his-71 using …
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Dissecting Mammalian Replication-Independent Chromatin Assembly — Biochemical and Structural Studies on the H3.3-Specific Histone Chaperones Hira and Daxx
… the high conservation of the four core histones, H3, H4, H2A and H2B, throughout all eukaryotes, histone variants have emerged with variable degree of divergence from their canonical counterparts. These variants are thought to expand the regulatory repertoire of chromatin. The histone replacement …
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Functional Analysis of Daxx In Tumorigenesis of Pancreatic Neuroendocrine Tumors and Embryonic Development
… is a histone chaperone specific to Histone 3.3 (H3.3). DAXX interacts with ATRX forming a chromatin remodeling complex, which deposits H3.3 into telomeric and pericentric region of the genome. The importance of Daxx was manifested in embryonic development. The loss of Daxx leads to early …
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Genomic and Molecular Characterization of Brainstem Glioma
… occurring in brain- stem gliomas exclusive of H3.3 mutations. We further revealed somatic mutations to PPM1D, the gene encoding for the Protein phosphatase 1D in 37.5% of brainstem gliomas harboring H3.3 mutations. We determined that PPM1D mutations are mu- tually exclusive with TP53 mutations …
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Using "Designer" Nucleosomes to Study Enzymatic Crosstalk Between Histone Ubiquitylation and Histone Methyltransferases
… on chromatin. It has been previously shown that H3 lysine 4 methyltransferases involved in transcriptional activation are stimulated and repressed by H2Bub and H2Aub, respectively. Here, using chemically-defined "designer" mononucleosomes, I tested whether nucleosomal H2BK120ub and H2AK119ub …
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Quiescence induces epigenetic changes in bovine fibroblasts and improves their reprogramming into cloned embryos
… hypomethylated at lysines (K) of histone 3 (H3), specifically H3K4me3, H3K9me2, H3K9me3 and H3K27me3, but not H3K9me1. Histone acetylation was reduced at H3K9 and H4K5, increased at H3K12 and remained unchanged at H3K16. G₀ cells also significantly reduced DNAme. In addition, they …
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Bioorthogonal Chemical Reporters Reveal Fatty-Acylation of Histone H3 Variants and Cholesterol Modification of Proteins and Trafficking in Cells
… proteins were found, in particular histone H3 variants. Histones H3.1, H3.2 and H3.3 were demonstrated to be modified with fatty acid chemical reporters on the conserved cysteine 110, a novel site of S-acylation on histone H3.2. This newly discovered modification of histone H3 variants could …
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The structural analysis of histone H3 lysine 56 acetylation and related histone chaperone complexes
… Factor 1 (CAF-1) is responsible for depositing H3.1/H4 tetramers in a DNA synthesis dependent manner. During replication independent assembly H3.3/H4 is deposited by Histone Regulator (Hir) complex. In both instances nascent histone H3/H4 dimers are supplied by Anti-Silencing Factor 1 …
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Characterizing and targeting the genomic consequences of ATRX deficiency in glioma
… revealed that CX-5461 disrupted histone variant H3.3 deposition, enhanced replication stress and DNA damage, activated p53-independent apoptosis, and induced G2/M arrest selectively in ATRX-deficient GSCs. These data were corroborated <em>in vivo</em>, where combinational treatment profoundly …
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Regulation of the oncogenic PIM1 kinase by SUMOylation
… protein kinase activity in vitro when Histone H3.3 was used a substrate, indicating that SUMOylation might enhance PIM1 kinase activity. Interestingly, both K169R and E171A displayed substrate specificity in vitro and when expressed in cells, suggesting that SUMOylation might govern PIM1 …
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Comprehensive Integrated Genomic and Histopathological Analysis of Paediatric Diffuse Intrinsic Pontine Glioma
… mutations in novel cancer drivers, histone H3 mutation in 78.5% and ACVR1 mutations in 21.25% of DIPG patients, respectively and discover that DIPGs are three molecularly distinct subgroups (H3-K27M, Silent and MYCN). The H3-K27M subgroup is highly p.Lys27Met histone H3 mutated and …