Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"H22"”.
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Generation and therapeutic evaluation of recombinant immunotherapeutics on CD64+ monocytic cell lines and polarized ex vivo differentiated human macrophages
… Methods: The CD64-targeting antibody fragment (H22(scFv)) was used to generate; 1) a recombinant immunotoxin (rIT) through genetic fusion with the enzymatic domain of Pseudomonas aeruginosa exotoxin A (ETA`), or 2) a SNAP-tag fusion protein (FP) to allow chemical coupling of the antibody …
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Physical mapping of an early sea urchin gene battery from Parenchinus angulosus
… and partial sequencing to be identical to H22, an early histone gene battery isolated from Psammechinus miliaris. (This latter gene was obtained from M. Birnstiel.) This was further confirmed by electron microscopy, and proved to be a convenient testing ground for the electron microscopic …
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Herstellung und Charakterisierung von rekombinanten, ETA-basierten CD64-Immuntoxinen zur Bekämpfung von dysregulierten Makrophagen bei chronischen Hautentzündungen
… (cutaneous)-inflammations. Both the monovalent H22(scFv)-ETA´ and the bivalent H22(scFv)2-ETA´ immunotoxin should be produced in E. coli, purified and characterized for their biological characteristics both in-vitro and in an transgenic animal model. The focus of this work is the comparison of …
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Entwicklung, Optimierung und Charakterisierung anti-CD64-basierter Immuntherapeutika
… anti-CD64 single chain antibody fragment (scFv) h22(scFv) and killed by ETA. However, the major drawback of such ITs is their potential immunogenicity. Repeated doses may cause hypersensitive reactions and lead to neutralization of the ITs by antibodies directed against the non-human domains. …
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Poly(glutamic acid) promoted assembly of nucleosome cores on the histone gene quintet of psammechinus miliaris
… also assembled on unique DNA, namely the intact h22 histone quintet of Psammechinus miliaris. Low resolution mapping, by indirect endlabelling of polycores assembled on the quintet, did not reveal any preferred sites of assembly. To investigate the core associated DNA at single base pair …
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Establishment of enhanced protein purification strategies for recombinant immunotherapeutics
… bacterially expressed recombinant immunotoxin 13 H22(scFv)-ETA. IMAC was employed as the initial purification step to recover the full-length histidine-tagged recombinant proteins. The Ni2+ immobilized on the matrices in columns has a high affinity for Histidine incorporated, which allows the …