Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Group II introns"”.
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Characterization of splicing mechanisms by single-molecule fluorescence
<p>Group II introns rank amongst the largest self-splicing ribozymes found in bacteria and organellar genomes of various eukaryotes. Despite the diversity in primary sequences, group II introns posses highly conserved secondary structures consisting of six domains (D1-D6). To perform its function, …
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DNA target site recognition by the Ll.LtrB group II intron RNP
Mobile group II introns are retroelements that site-specifically insert into DNA target sequences. The group II intron mobility pathway is mediated by a ribonucleoprotein particle (RNP) composed of excised intron RNA and an intron-encoded protein (IEP). The intron lariat inserts at a specific DNA …
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Genetic and biochemical analysis of a conserved DEAD-box helicase motif
… helicase required for efficient splicing of group I and group II introns, to probe the role of the conserved motif Va. We combine saturation mutagenesis, in vivo selection, and next-generation sequencing as part of a novel, high-throughput approach to identify functional mutants of motif Va …
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Characterization of the Group II Intron Gs. Int1 from the Thermophilic Bacterium <em>Geobacillus stearothermophilus</em>.
<p>Group II Introns are small segments of DNA that reside in the chromosome of bacteria or the organelles of primitive eukaryotes. These elements have some very interesting properties. First, they are retrotransposons that can move from one location to a new location in DNA via a reverse …
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In Vivo Studies of Yeast Mitochondrial Intron Splicing : Ectopic Branching and a Screen for Nuclear Encoded Splicing Factors
The splicing mechanism of group II introns is analogous to that of nuclear introns and it is generally thought that both share a common ancestor. This work contains two studies of group II intron splicing in yeast mitochondria. Previous studies done in collaboration with Dr. Anna Pyle at Yale …
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Fluctuations and response in complex biological systems: Watching stochastic evolutionary and ecological pattern dynamics
… and inactivation rate. This model explains why Group II introns are present in about 30% of bacterial species, while retrotransposons are essentially absent. This research sheds light on the early evolution of the eukaryotic spliceosome, the cellular machinery allowing complex organisms to …