Global ETD Search
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Showing 1 to 8 of 8 for “"Group II Intron"”.
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DNA target site recognition by the Ll.LtrB group II intron RNP
Mobile group II introns are retroelements that site-specifically insert into DNA target sequences. The group II intron mobility pathway is mediated by a ribonucleoprotein particle (RNP) composed of excised intron RNA and an intron-encoded protein (IEP). The intron lariat inserts at a specific DNA …
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Characterization of the Group II Intron Gs. Int1 from the Thermophilic Bacterium <em>Geobacillus stearothermophilus</em>.
<p>Group II Introns are small segments of DNA that reside in the chromosome of bacteria or the organelles of primitive eukaryotes. These elements have some very interesting properties. First, they are retrotransposons that can move from one location to a new location in DNA via a reverse …
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Characterization of splicing mechanisms by single-molecule fluorescence
<p>Group II introns rank amongst the largest self-splicing ribozymes found in bacteria and organellar genomes of various eukaryotes. Despite the diversity in primary sequences, group II introns posses highly conserved secondary structures consisting of six domains (D1-D6). To perform its function, …
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Single Molecule Studies Of Spliceosomal Snrnas U2-U6
… crystal</p> <p>structure of the self-splicing group II intron.19-16, 24b-17, 48 We proposed that these base</p> <p>triples existing in the spliceosomal RNA U2/U6 complex are in the same family of the</p> <p>ones found in crystal structure of the self-splicing group II intron given the …
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In Vivo Studies of Yeast Mitochondrial Intron Splicing : Ectopic Branching and a Screen for Nuclear Encoded Splicing Factors
The splicing mechanism of group II introns is analogous to that of nuclear introns and it is generally thought that both share a common ancestor. This work contains two studies of group II intron splicing in yeast mitochondria. Previous studies done in collaboration with Dr. Anna Pyle at Yale …
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Local and global investigations into DEAD-box protein function
… of Mss116p is probed in the folding of a cognate group II intron substrate, aI5[gamma], under near-physiological conditions. Three catalytically-active constructs of aI5[gamma] are used and catalysis serves as a proxy for folding. Folding of all constructs is promoted by the presence of Mss116p …
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Expression and Function of the Chloroplast-encoded Gene matK
… matK is the only proposed chloroplast-encoded group II intron maturase. There are 15 genes in the chloroplast that would require a maturase for RNA splicing. Six of these genes have introns that are not excised by a nuclear imported maturase, leaving MatK as the only candidate for processing …
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Evolution of the genus Aristolochia - Systematics, Molecular Evolution and Ecology
Evolution of Piperales – matK gene and trnK intron sequence data reveal lineage specific resolution contrast. Piperales are one of the largest basal angiosperm orders with a nearly worldwide distribution. The order includes three species rich genera, Piper (ca. 1,000 species), Peperomia (ca. …