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Showing 1 to 20 of 41 for “"GroEL"”.
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Structural biology of GroEL assisted protein folding
GroEL/ES is the classical example of molecular chaperone that assists the re-folding of many misfolded proteins (SP). Recent kinetic analyses revealed a new paradigm of how GroEL/ES uses ATP to assist protein folding. Following these pioneering biochemical studies, I address two fundamental …
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Allostery and GroEL: Exploring the Tenets of Nested Cooperativity
… cycle of the <i>E. coli</i> chaperonins GroEL and GroES, no model proposed to date accounts for all the effects seen experimentally by the various allosteric ligands: ATP, ADP, SP, GroES, and K+. The work in this dissertation explores the various allosteric transitions in the GroEL …
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Analogues of Nitrofuran Antibiotics are Potent GroEL/ES Pro-drug Inhibitors with Efficacy against Enterococcus Faecium, Staphylococcus Aureus, and Escherichia Coli
… essential to bacterial survival. The bacterial GroEL/ES chaperonin system is viewed as a viable antibiotic target. It has been proven essential to bacterial survival and homeostasis under all conditions. Previous studies identified hit GroEL/ES inhibitors with potential antibiotic activity. One …
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Function and Properties of groE Chaperonins in Bacterial and Mammalian Cells
… with a limited number of polypeptides, whereas GroEL is promiscuous as it binds and mediates the folding of many polypeptides. This feature makes GroEL an attractive protein for investigating various aspects of protein folding in eukaryotic cells because its substrate interaction is diverse. In …
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Untersuchung der Reaktionszyklen von Chaperoninen aus Escherichia coli und Thermoplasma acidophilum mit Hilfe der Neutronenkleinwinkelstreuung
… allosterischen Wechselwirkungen innerhalb von GroEL wurde die Lösungsstruktur einer „single-ring“-Mutante von GroEL untersucht. Die Strukturen von apo-single-ring-GroEL und ADP-Komplexen aus protoniertem singlering- GroEL und protoniertem GroES unterscheiden sich nicht oder nicht meßbar von den …
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Stress response of porphyromonas gingivalis
… were identified as homologs of E. coli DnaK and GroEL respectively by Western immunoblotting. On two-dimensional Western blots, two forms of DnaK and four forms of GroEL were identified due to their slightly different isoelectric points. dnaK and groEL gene homologs were identified in several P. …
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Assessment of the Expression of Brucella Abortus Heat Shock Protein, Groel, in Vaccinia Virus to Induce Protection Against a Brucella Challenge in Balb/C Mice
… mammalian species. In this work the B. abortus groEL gene encoding the antigenic heat shock protein GroEL was subcloned into vaccinia virus via homologous recombination. Expression of the GroEL protein in vaccinia infected cells in-vivo was confirmed by immunoblotting. Groups of 5 female BALB/C …
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Analysis of the multiple chaperonins of Mycobacterium smegmatis
… loss of the M. smegmatis Cpn60.1. While E. coli GroEL and Cpn60.3 appear to only partially complement, Cpn60.2 shows no complementing ability. Cpn60.2 expresses well and complements for loss of the E. coli Cpn60 homologue GroEL even at higher temperatures (42°C). Using native gels and analytical …
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Effects of co-expression of chaperone combinations on production of soluble plasmodial protein PfAdoMetDC in E. coli
… 4 (pBB542) encoding DnaK with DnaJ and GroEL; combination 5 (pBB542-Pf70JE) encoding PfHsp70 with DnaJ and GroEL; and combination 6 (pBB542-KPfJE) encoding KPf with DnaJ and GroEL. The effects of these combinations on the conformation of purified PfAdoMetDC protein was assessed by …
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Generation of Baculovirus-Brucella Abortus Heat Shock Protein Recombinants; Mice Immune Responses Against the Recombinants, and B. Abortus Superoxide Dismutase and L7/L12 Recombinant Proteins
… as adjuvants, were analyzed. Vaccinia virus-GroEL recombinant as priming immunogen, followed by baculovirus-GroEL-Ribi booster, was explored. Androstenediol, an immune up-regulator, was tested for its ability to induce resistance against challenge. None of the mice inoculated with individual, …
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Functional and structural characterisation of Mycobacterial Chaperonins
… Mycobacterium tuberculosis there are 2 distinct groEL homologues which encode the chaperonins Cpn60.1 and Cpn60.2, with the latter predicted to be the main house-keeping chaperonin. Phylogenetic analysis has revealed that the genes for the duplicated chaperonins diverged a long time ago. This …
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Tick-borne haemoparasite occurrence and Anaplasma bovis strain diversity in eastern rock sengis (Elephantulus myurus)
… additional rock sengi samples using 16S rRNA and GroEL assays, optimised for specific detection of the A. bovis-like (sengi) variant, in combination with Sanger sequencing and phylogenetic analysis. Genomic DNA extracted from 160 eastern rock sengi blood samples collected from the Goro Game …
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Floating gate engineering for novel nonvolatile flash memories
… with high density is discussed. Using protein GroEL to obtain well ordered high density nanocrystal pattern, a flash memory device with Ni nanocrystals buried in HfO₂ is demonstrated. For this technique, the nanocrystal size is restricted to the GroEL's central cavity size and the density is …
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DEVELOPMENT OF A SUBUNIT LAWSONIA INTRACELLULARIS VACCINE
… a result, the most immunogenic proteins – FliC, GroEL, ClpP, and YopN were selected for further study. Next, combinations of administration routes and adjuvants were explored to improve vaccine efficacy and maintain immunogenicity. Commercial adjuvants were selected based on past success in …
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High-Throughput Determination of Mycobacterium smegmatis Protein Complex Structures
… grid blotting on blue native (BN) PAGE using GroEL as a test protein. The hydrophobicity and charge of the EM copper grid was tested to find the optimal grid property for particle transfer. This established that particles of GroEL could be transferred from BN-PAGE onto an EM copper grid and a …
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Elucidating the Role of Toxin-Induced Microbial Stress Responses in Biological Wastewater Treatment Process Upset
… analysis techniques, the heat shock protein, GroEL, was found to be induced in activated sludge cultures exposed to perturbations of chemicals at all concentrations tested (cadmium, pentachlorophenol, and acetone) or heat stress. As total cadmium concentrations increased above 5 mg/L, there …
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Einfluss von Synbiotika auf die intestinale Mikrobiota gesunder Neugeborener
… (qPCR)-Verfahren, basierend auf dem single-copy groEL als phylogenetisches Zielgen, zur spezifischen Quantifizierung von zwölf Bifidobakterienspezies in humanen Fäzes entwickelt und validiert. Ergebnisse: Die supplementierte Säuglingsnahrung war gut verträglich und unterstützte eine gesunde …
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NON-FOULING AFFINITY PLATFORMS FOR PROTEIN IMMOBILIZATION IN ELECTRON MICROSCOPY
… capture of his-tag T7 bacteriophage, RplL, and GroEL from crude lysates, as well as purified nanodisc-solubilized his-malFGK2, on these NTA-modified grids with an exceptionally low level of adsorption by non-target proteins has been observed. Our data illustrates the utility of these grids for …
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Analysis of protein-protein interactions linked to the formation of a bacterial cytoskeleton in Mycoplasma pneumoniae
… (P115), MPN430 (GAPDH), MPN447 (HMW1), MPN573 (GroEL), MPN665 (EF-Tu). These results confirm the two-hybrid analyses of proteins interacting with HMW2. Eight of them (P1, P90, PdhB, GAPDH, HMW1, GroEL, EF-tu and the gene product of MPN297) were also found in the Triton X-100 insoluble fraction, …
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Elucidating aquatic virioplankton diversity and dynamics using high-throughput DNA sequencing
… EL operons, with the phylogeny of large-subunit GroEL genes matching the genomic context of the GroEL gene. Archaeal versions of chaperonins (i.e. thermosomes) were also discovered in viral metagenomes, revealing the presence of tailed archaeal viruses in surface seawaters. The phylogenetic …
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