Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 93 for “"Green fluorescent protein (GFP)"”.
-
Expression of green fluorescent protein (GFP) in Leishmania spp. to identify survival strategies the parasite imposes on the human host
… inside the host cells through the modulation of protein expression including cytokines. The changes involving Leishmania promastigote and host cells is well documented during the early stage of infection. However, little is known on the modulative effect of L. aethiopica and L. mexicana during …
-
Green fluorescent protein as a mechanical sensor
… structure dynamics. To this end, the green fluorescent protein (GFP) is considered as a mechanical sensor candidate with many desirable characteristics. Implicit solvent molecular dynamics CHARMM simulations demonstrated details inaccessible by AFM and OT methods, such as the linkage …
-
Single-molecule imaging studies of protein dynamics
… on single molecule studies of the dynamics of Green Fluorescent Protein: GFP) and other fluorescent-labeled proteins by utilizing Total Internal Reflection Fluorescence: TIRF) microscopy and imaging. The single molecule experiments of this thesis covered three broad topics. First, the …
-
Investigation of CNT-induced Escherichia coli Lysis and Protein Release
… BL21 (DE3) containing a plasmid that expressed Green Fluorescent Protein (GFP) and -lactamase were exposed to CNTs under various levels of agitation for different times. Fluorescence assay for GFP, optical absorbance for -lactamase activity, and Transmission Electron Microscopy (TEM) were used …
-
Molecular Dynamics Simulation Studies on Interfacial Dynamics of Proteins
Clusters of water molecules embedded in proteins or at the interface between globules mediate protein dynamics, allostery and charge transfer. Confined clusters of water molecules exhibit dynamics distinct from dynamics of water in the bulk or in the hydration layer around biomolecules. Here, we …
-
Identifizierung und Analyse der an der pflanzlichen Zellwandbiosynthese beteiligten Gene in Arabidopsis thaliana
… Methode der Identifizierung apoplastischer Proteine aus regenerierten Protoplasten von Arabidopsis thaliana. Prototoplasten bilden während ihrer Regeneration eine neue Zellwand aus, wobei sie eine sehr aktive Zellwandsynthese aufweisen. Achtzig Prozent der isolierten Gene konnten durch das …
-
Organosilicate nanoparticles as gene delivery vehicles for bone cells
… their phenotype. The expression of various proteins involved in bone formation, such as alkaline phosphatase, osteocalcin, osteopontin and fibronectin was quantified. The results indicated that osteoblasts retained their phenotype after organosilicate nanoparticle ingestion. The levels of …
-
Transformation of a Transposon Construct into Tomato for Functional Genomics Studies
… resistance to the antibiotic hygromycin and the green fluorescent protein (GFP) gene, which are driven by specific plant promoters. The selectable marker genes such as HYG and BAR were used to select the rare transformation events by making the transformed tomato tissue resistant to the toxic …
-
Effect of Emb-9 and Let-2 Mutations on the Proper Assembly of Basement Membranes and on the Morphology of Body Wall Muscle Cells in Caenorhabditis Elegans
… plasmid DNA containing the marker gene rol-6 and green fluorescent protein (GFP) into the gona of wild type animals heterozygous for the let-2 and emb-9 genes. Transgenic worms showed GFP expression outlining body wall muscle cells due to the presence of a myo-3 promoter, and β1 integrin secretion …
-
Isolation and characterization of CEABF-1, the ABF-1 homolog in C. elegans
… (http://www.ncbi.nlm.nih.gov) predicted a bHLH protein in C. elegans, located on cosmid ZK682.4, of 170 amino acids with an overall 51% similarity to human ABF-1. Importantly, it had a 72% similarity to human ABF-1 within the bHLH domain. Genomic and eDNA clones of CeABF-1 were isolated using …
-
Quantitative Characterization of Conserved Noncoding Regions by Flow Cytometry
… region critical to basic morphogenesis. CNR-green fluorescent protein (GFP) constructs can demonstrate spatial and temporal CNR activity by microscopy visualization. However, characterization of regulatory modules within a CNR requires quantitative, in addition to qualitative, analysis. The …
-
The photophysics of fluorescent protein chromophores
Fluorescent proteins of the Green Fluorescent Protein (GFP) family are important and versatile scientific tools used in a range of applications including the labelling of proteins, cells and tissue, high resolution imaging and studying protein-protein interactions. They exhibit a very diverse range …
-
Construction of green fluorescent protein plasmids for labeling of herbicide-degrading Delftia acidovorans MC1071
… The objective of my research is to construct two green-fluorescent protein (GFP) plasmids to tag a specific strain of D. acidovorans (MC1071) for visual distinction from one or more competing microbes in a microfluidic pore structure that contains spatially varying concentrations of 2,4-DP, 2,4-D, …
-
Mechanistic studies of a AAA+ protease
… energy-dependent degradation of most cytosolic proteins. Substrates for AAA+ proteases are unfolded and translocated into a compartmental peptidase. The requirement for protein unfolding raises several questions. How easily are proteins unfolded within the native environment of a cell? Are some …
-
Production and characterization of a phage endolysin with putative antimicrobial activity against Cutibacterium acnes
… the endolysin gene into vectors (pET11a & pBAD-GFP) for expression by Escherichia coli or as a fusion protein to green fluorescent protein (GFP). We compared the growth rates of C. acnes treated with cell lysate of cultures induced to produce the endolysin to non-treatment controls to evaluate …
-
Tuning DNA binding and gene expression using zinc finger proteins and engineered promoters
… To accomplish this, we used zinc finger proteins (ZFPs) as regulators of engineered promoters assayed by green fluorescent protein (GFP) as a fluorescent transcriptional reporter probe. In particular, we designed strong-binding three finger ZFPs as proof-of-principle regulatory elements, …
-
Development of a Transposon Based Activation Tagged Mutant Population in Tomato for Functional Genomic Analysis
… transposon tagging construct, Ac-DsATag-Bar_gosGFP, to produce activation tagged and knockout mutants in the processing tomato variety, M82. The construct contained hygromycin resistance (hyg), green fluorescent protein (GFP), and maize transposase (TPase) on the stable Ac element, along with a …
-
Stochastic modeling of inducible logical gates in Escherichia coli
… chemical inducers IPTG and aTc and produces green fluorescent protein (GFP) as an output signal. Experimentally, we broke down the AND gate promoter into its individual components by replacing the tet and/or lac operator sites by a non-binding E. coli DNA sequence. The individual components …
-
Ultrafast excited state reaction dynamics in light-driven unidirectional rotary molecular motors and fluorescent protein chromophores
… HBDI derivatives (synthetic chromophores of the green fluorescent protein (GFP)). In both cases, the excited state is selectively probed by ultrafast fluorescence up-conversion with a time resolution better than 50 fs. Molecular motors have biphasic (sub-picosecond and picosecond) fluorescence …
-
Fundamental differentiation and growth characterization of murine embryonic stem cells in varied culture conditions
… this research, an ES cell line with transfected green fluorescent protein (GFP) co-expressed by an Oct-4 promoter was analyzed by fluorescence-activated cell sorter (FACS) to obtain such profile. As Oct-4 is an ES cell differentiation marker whose expression varies with pluripotency, GFP …
Page 1 of 5