Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 193 for “"Green Fluorescent Protein"”.
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Green fluorescent protein as a mechanical sensor
… structure dynamics. To this end, the green fluorescent protein (GFP) is considered as a mechanical sensor candidate with many desirable characteristics. Implicit solvent molecular dynamics CHARMM simulations demonstrated details inaccessible by AFM and OT methods, such as the linkage …
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The Electronic Excited States of Green Fluorescent Protein Chromophore Models
… the excited states of model chromophores of the green fluorescent protein of A. victoria. We calculate several low-lying states by several methods of quantum chemical calculation, including state-averaged complete active space SCF (CASSCF) methods, time dependent density functional theory …
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Expression and Localization of Green Fluorescent Protein in B. abortus strain RB51
… antigens and produce a response. Expressing a protein in different bacterial compartments has been shown to affect its accessibility to the immune system and the way the antigen is processed by antigen presenting cells. In order to determine if the immune response is affected by the …
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Site-specific immobilization of tetrazine-modified green fluorescent protein on trans-cyclooctene surfaces
The ability to control the orientation of proteins attached to a surface, one of the goals of this work, has applications within areas such as microarrays, biomaterials, tissue engineering and biosensors. Non-covalent protein adsorption (physisorption), the easiest of all protein-attachment …
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Receptor Mediated Oral Delivery Of Bioencapsulated Green Fluorescent Protein Expressed In Transgenic Chloroplasts
… be minimized or eliminated when therapeutic proteins are expressed and orally delivered via plant cells. Chloroplasts have the machinery to fold complex and biologically active eukaryotic proteins in the soluble chloroplast stromal compartment. Protein expression through chloroplast …
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Construction of green fluorescent protein plasmids for labeling of herbicide-degrading Delftia acidovorans MC1071
… The objective of my research is to construct two green-fluorescent protein (GFP) plasmids to tag a specific strain of D. acidovorans (MC1071) for visual distinction from one or more competing microbes in a microfluidic pore structure that contains spatially varying concentrations of 2,4-DP, 2,4-D, …
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Towards gene therapy for cystic fibrosis: enhanced green fluorescent protein as a reporter of promoter activity
Cystic Fibrosis (CF) is the most common lethal inherited disease, affecting -1/2000 live births. Although the genetic lesion, a mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, has been elucidated, the exact mechanism whereby this causes the debilitating disease …
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Assessment of Murine Embryo Development Following Electroporation and Microinjection of a Green Fluorescent Protein DNA Construct
… microinjection of condensed or linear green fluorescent protein DNA into murine embryos using electroporation. In experiment 1, the effect of embryo orientation (group or linear) within the electroporation chamber on development was evaluated using zygotes which received one pulse …
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The Potential for Green Fluorescent Protein as a Screening Tool in the Production of Haploid Potato Plants
… 4404 containing plasmid pHB2892 with genes for green florescent protein (GFP) and kanamycin resistance. Hemizygous primary transformants (To) were produced from three leaf discs: 17 diploid plants from one leaf disc, three and nine tetraploids from the other two leaf discs. GFP expression was …
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Characterization of the Biological Activities of Recombinant Fusion Protein Green Fluorescent Protein/Human Zona Pellucida Protein 3 (GFP/HZP3)
… application of a pEGFP expression vector, fusion protein GFP/ZP3 was successfully generated and expressed. The expression of GFP/ZP3 was evidenced by RT-PCR and western blot. The fusion protein was partially purified by Ni-NTA affinity column from cell culture medium of stably transfected cells …
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Expression of green fluorescent protein (GFP) in Leishmania spp. to identify survival strategies the parasite imposes on the human host
… inside the host cells through the modulation of protein expression including cytokines. The changes involving Leishmania promastigote and host cells is well documented during the early stage of infection. However, little is known on the modulative effect of L. aethiopica and L. mexicana during …
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Recombinant Salmonella enterica serovar Typhimurium vaccine vector expressing green fluorescent protein as a model antigen or human immunodeficiency virus type 1 subtype C Gag
Includes bibliographical references (leaves 172-200).
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Spatial-Temporal Regulation of the Atonal Homolog 1 Gene
… 5' and 3' of atonal homolog 1 and assaying for green fluorescent protein expression in transgenic mice. Transgenic embryos were analyzed at embryonic day 10.5 in the neural tube, metencephalon and rhombencephalon and at embryonic day 16.5 in merkel cells, inner ear cells, vestibular cells and …
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The photophysics of fluorescent protein chromophores
Fluorescent proteins of the Green Fluorescent Protein (GFP) family are important and versatile scientific tools used in a range of applications including the labelling of proteins, cells and tissue, high resolution imaging and studying protein-protein interactions. They exhibit a very diverse range …
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New methodologies for studying the biological activity of platinum complexes
… importance of high mobility group (HMG) domain proteins in the cytotoxicity of cisplatin. Platinum compounds were evaluated based on their ability to form DNA adducts that bind specifically to HMG-domain proteins. A mixture of platinum amino acid complexes was prepared and combined with DNA to …
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Expression of Foreign Genes in the Pseudomonas Bacteriophage Pf3
… assuming that promoters for the phage major coat protein (MCP) gene would also promote the expression of any foreign gene inserted downstream of the MCP gene. As proof of principle, the bacteriophages Pf3, Pf1, and M13 were engineered in this way to successfully synthesize Enhanced Green …
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GFP- coated microparticles to quantify and compare wild- type desmin with known desmin mutations in human heart disease via quantitative live-cell fluorescence Imaging
… for reproducible processing and quantifying by fluorescent imaging. The current work provides a novel approach to facilitate the quantification in-vivo using conventional fluorescence microscopy combined with customized Poly (methyl methacrylate) (PMMA) microparticles developed for this purpose. …
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Fate of Salmonella Typhimurium in biofilms of drinking water distribution systems
… using the pUT mini–Tn5 Km transposon with the green fluorescent protein gene gfpmut3b*. Southern Blot hybridisation confirmed that the gfp gene had integrated into the chromosome. The gfp gene was stably maintained and the gfp-labelled recombinants were not growth rate impaired under low …
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Impatiens Necrotic Spot Virus Resistance in Transgenic Impatiens walleriana and Lycopersicon esculentum
… with Agrobacterium. The construct contained GFP (green fluorescent protein) and hygromycin antibiotic-resistant selectable markers, and the antisense sequence of open reading frame of INSV nucleocapsid protein (N). The N gene is expected to confer INSV resistance by RNA interference or gene …
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