Global ETD Search
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Showing 1 to 20 of 23 for “"Grb2"”.
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Evaluating The Therapeutic Efficacy of Grb2 Inhibition In Ovarian Malignancies
… such as growth factor receptor-bound protein-2 (Grb2) play important roles in cancer cell signaling. In the present study, we examined the biological effects of liposomal antisense oligodeoxynucleotide that blocks Grb2 expression (L-Grb2) in ovarian cancer models.</p> <p><strong>Experimental …
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The Role of Adaptor Protein Grb2 in Ecotropic Retrovirus Entry and Receptor Trafficking
… growth factor receptor-bound protein 2 (Grb2), as a key protein for eMLV infection. Grb2 is an adaptor that has been shown to couple cell surface receptors such as the epidermal growth factor receptor (EGFR) and hepatocyte growth factor receptor (Met) to intracellular signaling events. …
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Competition Between Grb2 and Plcγ1 For Binding to Fgfr2 Regulates Phospholipase and Pten Activity, Leading to Cell Invasion and Proliferation
… domain of Plcγ1 competes with the SH3 domain of Grb2 for binding to FGFR2 in a concentration-dependent manner. Consequently, reduction of cellular concentrations of Grb2 lead to receptor-dependent recruitment of unphosphorylated Plcγ1. Bringing of the phospholipase to the membrane in this way …
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Protein-Protein Interactions and Muscle cell Signaling Via Syntrophin
… a-, b-dystroglycan, and syntrophins all bind Grb2, the growth factor receptor bound adapter protein. Mouse a1-syntrophin chimeric fusion proteins bind Grb2 in a Ca<sup>2+</sup>-independent manner. This binding was localized to two proline rich sequences near the N terminal PH1 domain. This …
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Functional relationships among lck kinase and the lat and grb2 adaptors in the intracellular signaling cascade of the tcr/cd3 complex: implications in thymic development and lymphocyte activation
… in early TCR signaling, the LAT adaptor and Grb2. In the first chapter, we describe a new cell line (termed J.CaM1.7 cells) derived from J.CaM1.6 cells that happens to be doubly deficient in LAT and Lck. These cells require simultaneous expression of LAT and Lck to recover intracellular …
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Mechanistic and Functional Characterization of B and T Lymphocyte Attenuator (Btla) In Cd8 Tumor Infiltrating Lymphocytes
… and ITSM motifs of BTLA are well described, the Grb2 motif’s function remains understudied.</p> <p>In this study, we sought to determine the functional characteristics of the CD8<sup>+</sup>BTLA<sup>+</sup>TIL subset and define the contribution of the Grb2 motif of BTLA in T cell co-stimulation. …
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INTERACTIONS OF ADAM15 SPLICE VARIANTS WITH SH3 DOMAIN PROTEINS
… the ADAM15 ICD variants and the SH3 domains of Grb2, Src and Brk. Chemical Shift Perturbations (CSPs) indicated that Grb2 interacted with all ADAM15 ICD variants via residues 22Glu, 44Trp and 56Phe. Analysis of the common regions of the ICD variants implicated the polyproline region 4 in this …
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Identifizierung und Charakterisierung von Interaktoren des zellulären Prion-Proteins
… zu charakterisieren: Pint1, Synapsin Ib und Grb2. Mittels Copräzipitation konnte bestätigt werden, dass auch in Säugetierzellen eine physiologische Wechselwirkung zwischen den identifizierten Interaktoren und PrP auftritt. Das Protein Pint1 wurde bislang noch nicht beschrieben und besitzt …
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Pharmacological insights into C-‐C motif chemokine receptor 5 mediated chemotaxis
… involved in chemotaxis. Inhibition of dynamin, Grb2 and non-‐receptor tyrosine kinases were equally effective at blocking migration in THP-‐1 cells but less effective at blocking CXCL11 stimulated migration in activated PBLs. Conclusions: This study has shown that CCL3 stimulated chemotaxis …
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Regulation of hepatocyte growth factor receptor endocytic trafficking
… Recruitment of the adaptor protein Grb2, but not Gab1, was essential for ligand induced HGFR internalization. I then showed Cbl, an E3 ubiquitin ligase recruited by Grb2 to HGFR, played an essential role in receptor internalization. Furthermore, the E3 ligase activity of Cbl and …
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Regulation of inflammatory TLR signalling by the BCAP adaptor
… screen identified a number of proteins including Grb2 and CRKL as new adaptor proteins in the BCAP interactome. Subsequent validation in mammalian cells via co-immunoprecipitation showed that Grb2 is a direct interaction partner of BCAP. Further characterization of all SH2 and SH3 …
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Ein neuer und ungewöhnlicher Signalweg erlaubt physiologischen Konzentrationen von Erythropoetin mitogene Kinasen in primären erythroiden Vorläuferzellen zu aktivieren
… (Shc), „growth factor receptor bound protein 2“ (Grb2), „son of sevenless“ (Sos), Ras, Raf, MEK und Erk erfolgt, sondern durch einen PI3K-abhängigen und Raf-unabhängigen Signalweg, welcher PKC-Aktivität benötigt, reguliert wird.
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Targeting the protein tyrosine phosphatase, SHP2, and PI3K in FLT3-ITD+ leukemia
… cells is through decreased recruitment of Grb2, which participates with son of sevenless, SOS, to activate the RAS-Erk signaling pathway. The lack of improvement in overall survival could be due to preserved STAT5 signaling, as observed during in vitro experiments. Collectively, these data …
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Genexpression des Adaptorproteins Shc bei Patienten mit Juveniler Myelomonozytärer Leukämie (JMML)
… Rezeptor und dem Ras-aktivierenden Komplex Grb2/Sos. In Tumorzellinien erwies sich Shc als ein limitierender Faktor bei der GM-CSF vermittelten Signaltransduktion. Wurden Zellen mit Shc transfiziert, übte GM-CSF einen höheren Proliferations- und Differezierungsreiz auf diese Zellen aus. <br> …
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The Role of p66Shc in Stem Cells: Signalling, Identity and Apoptosis
… which p66Shc acts as a competitive inhibitor of Grb2 recruitment, restraining ERK–STAT3 signalling to promote lineage priming. In NSCs, p66Shc loss conferred resistance to apoptosis induced by epidermal growth factor (EGF) withdrawal, EGF-receptor inhibition, or MEK inhibition, but not to …
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Characterizing the Phosphorylation State of Tie2 using SH2 Domain Fusion Proteins
… systems using the SH2 domains of Shp2 N-C and Grb2. As expected, phosphorylation of Tie2 in the presence of its orphan receptor Tie1 was attenuated compared to wild-type levels. Based upon available data, we anticipate this method as a useful tool to assess the phosphorylation state of Tie2 and …
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Mesothelial and Mural Cell Contribution to Vascular Development through PDGF Signaling
… activation includes PI3 kinase, Src, RasGAP, Grb2, Shp-2, and PLC?gamma for the regulation of cellular functions.The focus of this research was to determine the temporal and functional requirements of PDGFR signaling in mural cells. To address the temporal requirements for PDGFR beta, genetic …
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Dissecting mechanisms and consequences of oncogenic RTK fusion signaling
… by sequestering RTK adapter proteins including GRB2 and SOS1. Furthermore, ALK inhibition, while suppressing oncogenic signaling, simultaneously releases sequestered adapters and thereby resensitizes RTK signaling. Resensitized RTKs promote rapid and pulsatile ERK reactivation originating from …
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Investigation of the molecular mechanisms underlying the invasive phenotype in a panel of lung cancer cell lines
… motility and differentiation. We chose GRB2, RAN, MIF and ANXA2 gene targets which were differentially downregulated due to mir-29a overexpression and performed siRNA induced knockdown in DLKPA cell line. Results revealed RAN to be anti-invasive and anti-proliferative in our cell line …
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The intramolecular domain interactions and phosphatase activation mechanisms of Shp2
… of Y580, which is then sequestered by Grb2 in cells. These results suggested that the cellular environment can tune and switch the interaction of C-SH2 toward pY542. When the sequences containing Y542 and Y580 were engineered to exchange their positions such that the favored sequence …
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