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Showing 1 to 13 of 13 for “"Gram-stain"”.
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Development of A Molecular Gram-Stain Assay For The Diagnosis of Blood Stream Infections Associated With Sepsis
… annually. Currently, blood culture followed by Gram-stain analysis is the gold standard for diagnosing bacterial infections associated with sepsis. This method generates a high rate of false negative results and, in general, requires 20 to 48 hr to provide results. Both of these problems are …
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The Pneumococcus Urinary Antigen Test Kit: Use in the laboratory for the presumptive diagnosis of pneumococcal bacteraemia
… on selected blood cultures with appropriate Gram stain results, immediately after signalling positive for the rapid identification of S. pneumoniae bacteraemia. Method: We collected 212 blood cultures that had signalled positive with an automated blood culture system, and then yielded …
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The Effectiveness of Virtual Simulations Impacting Conceptual Knowledge and Proficiency of Laboratory Techniques in an Undergraduate Microbiology Laboratory Course
… an oil immersion microscope and conducting a Gram stain. Undergraduate students (n=280) enrolled in a general microbiology laboratory course at South Dakota State University were investigated regarding effectiveness of virtual labs impacting conceptual knowledge and lab technique proficiency. …
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Diet Induced Alteration of the Murine Intestinal Microbiome After Antibiotic Ablation
… We detected a dominant non-cultivatable gram-positive coccobacillus associated with cultivatable yeast in feces of mice treated with a cocktail of broad-spectrum antibiotics while being fed a partially defined mouse chow that is commonly used for nutritional studies. The goal of this …
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Isolasi dan identifikasi bakteri rhizobium dari akar tanaman alfafa (Medicago sativa L.)
… dengan beberapa uji yaitu: uji pewarnaan gram, uji pewarnaan endospora, uji katalase yang selanjutnya dilakukan uji Microbact 12B. Hasil uji pewarnaan gram diperoleh 5 isolat bakteri berbentuk batang dan bulat dengan gram negatif. Pada uji katalase kelima isoalat dengan hasil katalase …
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Isolation and Characterization of Chitinases From Dasypus Novemcinctus, the Nine-Banded Armadillo
… were characterized using oxygen requirements, Gram stain reaction, and various other physiological tests. Bacterial isolate 8SI, isolated from the small intestine, produced an extracellular chitinase which has optimal activity at a pH of 5.0 and 40° C. The chitinase from isolate 8SI was …
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Assessment of Antimicrobial Resistance in Pathogens Responsible for Causing Bovine Mastitis in Kentucky
… positive for growth were identified using the gram stain and various biochemical tests. After identification, resistance to 11 antimicrobial agents was assessed using the Kirby-Bauer test. <em>Staphylococcus aureus</em> was found to be the most common species causing bovine mastitis, which was …
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A comparison of Probiotic and Standard yogurt based on branding (premium and basic brands), consumer preference, sensory evaluation, microbiological and nutritional analysis.
… analysis (such as total viable counts and gram stain), nutritional analysis (such as macro and micro nutrient compositions), consumer preference analysis (by questionnaire), and sensory analysis (by blind tasting) were conducted using the yogurts purchased. SPSS and Microsoft excel were …
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Characterization of the vaginal microflora
… i.e. the strictly anaerobe and very fastidious Gram positive coccus Atopobium vaginae. Also Dialister, Leptotrichia and Megasphaera species were commonly found in women with bacterial vaginosis. Another research group (77) found that several unknown bacteria in the Clostridiales order were found …
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The use of molecular diagnostic methods to improve the detection of the common bacterial and viral causes of community acquired meningitis in children in South Africa
… the screened 291 CSF samples, 7 (2.4%) cases Gram stain results were obtained along with relatively few cases with positive bacterial culture growth 4/291 (1.4%). Based on bacterial qPCR results, 8 (2.7%), 3 (1%) and 1 (0.3%) were positive for S. pneumoniae, N. meningitidis and H. influenzae …
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Ornithobacterium hominis isolates from a South African birth cohort
Ornithobacterium hominis is a gram-negative bacterium that colonises the infant nasopharynx. Ornithobacterium hominis 16S rRNA gene sequences have been identified in South-East Asia, Australia, Gambia and Kenya; however, this species is notably absent from cohorts based in the northern hemisphere. …
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A study of Door Pulls on Isolation Carts in a General Hospital for Evidence of Contamination
… of the organisms were noted. These smears were stained by the gram stain method and examined under the microscope. The gram positive micrococci were classified by streaking them on mannitol salt agar. A coagulase test was run on those organisms that fermented the mannitol.</p> <p>Sixteen …
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Effect of progestin-based hormonal contraceptives on genital inflammation and Th17 cell activation in adolescents at high risk for HIV infection
… Nugent scoring), and yeast (visible hyphae on gram stain) at all study visits. Data on relative abundance of vaginal microbial community types (CTs) from ectocervical swabs was available for this study, determined by 16S rRNA sequencing of the V4 region. Several genital samples were collected, …