Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Golgi Transport"”.
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ER to Golgi transport during apoptosis
The Golgi complex, a single -copy organelle, is composed of a ribbon of flattened stacks of cisternae during interphase. However, during apoptosis the Golgi is shown to fragment and disperse throughout the cytoplasm. Immunoelectron microscopy identified these fragments as vesicular-tubular …
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THE ROLE OF P24 PROTEINS IN THE ER TO GOLGI TRANSPORT OF TOLL-LIKE RECEPTORS
… secretory pathway, are involved in the ER to Golgi transport of TLRs. Protein interaction studies performed in HEK293T cells by Proximity Ligand Assay (PLA) reveal that TMED7 interacts with TLR2 and TLR4, but not with TLR3. In contrast, TMED2 interacts with TLR2, TLR4 and TLR3. Different TLRs …
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Structural and functional analysis of yeast proteins involved in ER-to-Golgi transport: Sec24p family proteins and the GTPase activating protein Gyp5p
… Zellen eine ausgefeilte intrazelluläre Transportmaschinerie evolviert, mit deren Hilfe Biomoleküle zu ihren jeweiligen Bestimmungsorten gebracht werde können. In der vorliegenden Arbeit werden einige der Mechanismen beschrieben, die den Transportschritt und die komplizierte Maschinerie …
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Novel regulators of trafficking in the yeast Golgi-endosomal system
… into the molecular mechanisms that direct post-Golgi trafficking events in the budding yeast Saccharomyces cerevisae. However, a key event in this process, the formation of secretory vesicles at the Golgi and sorting of cargo into these transport carriers, remains poorly understood. It has been …
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FUNCTIONAL CHARACTERIZATION OF DENND5A/RAB6IP1 IN EARLY EMBRYONIC DEVELOPMENT
… small GTP-binding protein that involves in intra-Golgi transport. In a yeast two hybrid screen by Bruno Goud group in 1995, they used Rab6 as bait and found several interacting cDNA clones including Rab6 interacting protein 1 or Dennd5a. Several Dennd5a interaction partners have been discovered, …
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The Arf GTPase exchange factor Sec7p interaction network:
The Golgi apparatus is the main crossroad of the intracellular trafficking network in all eukaryotic cells and plays a crucial role in the distribution of cellular material. To ensure the proper sorting and delivery of cargo proteins to their destination while maintaining Golgi homeostasis the …
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Genetic and Chemical Genetic Approaches to Shiga Toxin Inhibition
… inhibitors of Shiga toxin intracellular transport, as Shiga toxin is known to undergo a stepwise progression through host cells following endocytosis. This screen identified three compounds with distinct effects on Shiga toxin transport. A detailed characterization of one of these …
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Pharmacological Chaperones of the Dopamine Transporter: A Strategy for Increasing Function of Wild Type and Mutant Transporter
The dopamine transporter (DAT) is a membrane protein that is essential for regulating signaling and intracellular stores of the neurotransmitter dopamine. An array of pathological conditions have been linked to mutations in the dopamine transporter gene, including ADHD, bipolar disorder, autism, …
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The role of USP17 in lysosome trafficking and lysosomal function
… upon Sec31A could indicate an impact upon ER-to-Golgi transport, but again, a clear mechanism is not apparent. Finally, USP17 is shown to be involved in the RNF26-based network which can regulate lysosome positioning via the ubiquitination of p62 to tether vesicles to the ER, and in particular …
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From the Endoplasmic Reticulum to the Golgi Apparatus : In Vitro and In Vivo Approaches to Understanding COPII Vesicle Function in Plant Cells
The Endoplasmic Reticulum (ER) to Golgi transport is mediated by COPII vesicle in yeast and mammalian cell. COPII coats consist of the small GTPase Sar1p and the heterodimeric protein complexes Sec23/24 and Sec13/31. COPII mediated sorting occur when protein cargoes exit the ER. Although the …
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Defining the Cellular and Molecular Basis of Spondyloepiphyseal dysplasia tarda
One third of the proteome is transported in membrane-encapsulated vesicles from the endoplasmic reticulum (ER) to the Golgi apparatus en route to the plasma membrane, other intracellular organelles and the extracellular matrix. After folding, newly synthesized proteins reach the Golgi apparatus …
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Untersuchung von Proteinkomponenten der ER-Golgi Recycling-Maschinerie von Hefe
… alle bisher untersuchten ER-Proteine, die zum Golgi gelangen, mit Hilfe von COPI-Vesikeln zum ER zurücktransportiert. Bisher ist aber noch ungeklärt, wie die unterschiedlichen Proteine in die Vesikel dirigiert werden.Durch Verwendung eines Sec22-a-Hybridproteins konnte in unserer Arbeitsgruppe …
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Luminal calcium regulates membrane fusion in the early secretory pathway
… expansion of the endoplasmic reticulum to Golgi intermediate compartment (ERGIC) in the periphery of the cell visualized using the markers rbet1 and p24. Depletion of luminal calcium also results in major kinetic defects of VSV-G transport to the Golgi, as well as reconstitution of the …
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Luminal calcium regulates membrane fusion in the early secretory pathway
… expansion of the endoplasmic reticulum to Golgi intermediate compartment (ERGIC) in the periphery of the cell visualized using the markers rbet1 and p24. Depletion of luminal calcium also results in major kinetic defects of VSV-G transport to the Golgi, as well as reconstitution of the …