Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Glycosylphosphatidylinositol"”.
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Biosynthesis and Characterization of Early Glycosylphosphatidylinositol Anchor Intermediates in Saccharomyces Cerevisiae
Next, it was desired to obtain structural information on in vivo GPI anchor intermediates. To address this issue, I exploited the fact that at non-permissive temperature, the yeast dpm1--6 mutant accumulates GlcNH2-(acyl-inositol)-Pl. This precursor molecule is the earliest biosynthetic …
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Isolation and molecular genetic analysis of conditionally lethal yeast mutants in glycosylphosphatidylinositol anchoring
Glycosylphosphatidylinositol (GPI) anchors are a recently described class of glycolipids attached through covalent linkage to protein. To explore the functions of GPIs, I investigated the effects of GPI anchoring deficiencies in the unicellular eukaryote Saccharomyces cerevisiae.
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Development And Optimization Of The First High Throughput In Vitro Fret Assay To Characterize The Saccharomyces Cerevisiae Gpi-T
… Doctor of Philosophy</p> <p>The enzyme glycosylphosphatidylinositol transamidase (GPI-T) mediates the attachment of a glycosylphosphatidylinositol (GPI) anchor to the C-terminus of specific proteins to produce GPI anchored proteins. This post-translational modification is essential for …
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Prion protein in health and disease
… glycoprotein tethered to cell membranes by a glycosylphosphatidylinositol anchor. In mammals, PrP is expressed in many tissues, most abundantly in brain, heart, and muscle. Importantly, PrP is required for prion diseases, which are neurodegenerative diseases associated with misfolding and …
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Solution and solid-phase synthesis of potential carbohydrate vaccines for leishmaniasis and malaria
… in the host following red blood cell rupture. A glycosylphosphatidylinositol (GPI) anchor of parasite origin was recently identified, and had the properties of a toxin. The synthesis of a modified version of the malarial GPI both in solution and on solid-phase in an automated fashion is …
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Molecular mechanisms of synaptic proteins
… were explored for MDGA1 (MAM domain-containing glycosylphosphatidylinositol (GPI) anchor 1. In the following chapters, the SAMs neurexins (NRXNs), neuroligins (NLGNs), MDGAs, and immunoglobin superfamily 21 (IgSF21) are introduced. In our studies, we characterize how the structure-function …
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Molecular mechanisms of synaptic proteins
… were explored for MDGA1 (MAM domain-containing glycosylphosphatidylinositol (GPI) anchor 1. In the following chapters, the SAMs neurexins (NRXNs), neuroligins (NLGNs), MDGAs, and immunoglobin superfamily 21 (IgSF21) are introduced. In our studies, we characterize how the structure-function …
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Studies on the mechanisms that contribute to the endoplasmic reticulum quality control system in Saccharomyces cerevisiae
… candidates involved in protein glycosylation, glycosylphosphatidylinositol-anchored protein maturation and quality control. These results represent a strong starting point to gain further insight in how the processes necessary for proper ER homeostasis are interrelated.
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The localization of UCH-L1(Ubiquitin C-terminaHydrolase-L1) in lipid rafts of neuronal cell
… and slowness of movement. Lipid rafts enriched glycosylphosphatidylinositol (GPI)-anchored proteins, glycosphingolipid and cholresterol, are specialized microdomains that play a role in endocytosis, exocytosis, signal transduction and receptor traffic king. The disruption of lipid raft from …
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Macrophage Response to Interleukin-1 Stimulation and the Nature of Cell Surface Interleukin-1 Alpha
… through the type II IL-1 receptor (IL-1R2) and glycosylphosphatidylinositol (GPI) anchors. IL-1R2 is responsible for majority of the csIL-1α binding to the cell membrane with a small portion of csIL-1α being tethered to a GPI- associated protein. We report that csIL-1α is significantly …
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pH MODULATION OF FIBRIL DISSOCIATION AND COPPER BINDING PROPERTIES OF THE PRION PROTEIN
… glycoprotein attached to lipid rafts via its glycosylphosphatidylinositol anchor. Conversion of PrP<sup>C</sup> to its "scrapie" conformer (PrP<sup>Sc</sup>, the fibrillar form) constitutes the key event of the etiology of prion diseases. Fibril dissociation is necessary for efficient …
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pH MODULATION OF FIBRIL DISSOCIATION AND COPPER BINDING PROPERTIES OF THE PRION PROTEIN
… glycoprotein attached to lipid rafts via its glycosylphosphatidylinositol anchor. Conversion of PrP<sup>C</sup> to its "scrapie" conformer (PrP<sup>Sc</sup>, the fibrillar form) constitutes the key event of the etiology of prion diseases. Fibril dissociation is necessary for efficient …
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APP∙CNTN Complex Architecture is Conserved Throughout the Vertebrate Lineage
… The C-terminal FN-4 domain terminates with a glycosylphosphatidylinositol (GPI) anchor in the cell membrane; meaning that these GPI-anchored proteins have no intracellular components and thus require binding partners to communicate their interactions with other proteins on cell surfaces. CNTNs …
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Investigation of Trans-factors responsible for the developmentally linked instability of a highly regulated mRNA in Trypanosoma brucei
… on the regulatory 3’ untranslated region of the glycosylphosphatidylinositol-specific phospholipase C (GPI-PLC) mRNA, highly unstable in the procyclic developmental stage, was used in an RNAi library screen. A number of factors potentially responsible were identified, of which six were positively …
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Induction of Anopheles stephensi nitric oxide synthase by Plasmodium-derived factor(s)
… my studies, I have determined that P. falciparum glycosylphosphatidylinositol (PfGPIs) can induce AsNOS expression in A. stephensi cells in vitro and in the midgut epithelium in vivo. Based on related work in mammals, I hypothesized that parasite-derived AsNOS-inducing factors signal through the …
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Biochemical And Structural Characterization Of The Core Subunits Of Gpi Transamidase
… <p>Degree: Doctor of Philosophy</p> <p>Glycosylphosphatidylinositol transamidase (GPI-T) is a complicated, membrane-bound, multi-subunit enzyme that catalyzes an essential post-translational modification. This enzyme attaches GPI anchors to the C-termini of various proteins that contain …
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Discovery of IKBKE and CD24 siRNAs for the treatment of triple-negative breast cancer
… levels of CD24, which is a heavily glycosylated glycosylphosphatidylinositol (GPI)-anchored surface protein and plays an important role in tumor growth, invasion, and metastasis. Moreover, overexpression of CD24 in tumors is associated with resistance to therapies. Small interfering RNA (siRNA) …
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Syncytiotrophoblast formation is influenced by genes involved in lipid biology
The placenta develops during pregnancy to support the nutritional demands of the embryo. In the murine placenta, nutrient and waste exchange occurs in the labyrinth, the syncytiotrophoblast (SynT) cells are the key cell type of this region. Multinucleated SynT cells are formed through the process …
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Synthesis Of Functionalized Gpi Anchors And Related Glycoconjugates
<p>This dissertation is divided into two chapters describing the background, significance, and synthesis of GPI anchors and GPI-anchored molecules. GPI anchors are a class of complex glycolipids with the primary purpose of attaching cell surface proteins/glycoproteins to the cell membrane. The …