Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Glycosphingolipids"”.
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Structure-Activity Studies of Glycosphingolipids as Antigens of Natural Killer T Cells
Glycosphingolipids (GSLs), composed of a polar saccharide head and a lipophilic ceramide tail, are ubiquitous components of the plasma membrane of eukaryotic cells. They serve in many regulatory capacities and have antigenic properties towards natural killer T (NKT) cells of the innate immune …
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Advances in Fast Atom Bombardment Mass-Spectrometry (Glycosphingolipids, Nucleotides, Markers, Negative Ion)
… sensitivity and applicability. A series of glycosphingolipids and a series of protected oligonucleotides of known structure were analyzed to ascertain the potential utility of fast atom bombardment mass spectrometry in the structural elucidation of novel compounds in these classes. Negative …
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A Deuterium Nuclear Magnetic Resonance Study of Phospholipid and Glycosphingolipid Headgroup Structure
… (NMR) experiments with various phospholipids and glycosphingolipids are used to derive structural and motional information about these molecules within lipid bilayers. Reasonable models of motion are inferred from various experimental results, and calculated NMR parameters are determined for all …
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Regulation of Galactosylceramide Biosynthesis
… (GalCer), which are precursors of all mammalian glycosphingolipids. Relatively few studies have focused on this branchpoint because these monohexosylceramides are somewhat difficult to resolve chromatographically and because molecular biology tools have only recently become available to follow …
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Potential Modifications to Enzyme Replacement Therapy in Anderson-Fabry Disease
… is associated with a progressive deposition of glycosphingolipids, including globotriaosylceramide (GB3), galabioasylceramide, and blood group B substance in the cell. In affected males, and in some females, this leads to early death due to occlusive disease of the heart, kidney, and brain. This …
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Role of Membrane Contact Sites in the Neuropathogenesis of GM1-Gangliosidosis
… transport. These two lipids, along with glycosphingolipids, are the major lipid constituents of membranes; however, very little is known about the role of glycosphingolipids in MCS dynamics. Using a mouse model deficient for the lysosomal enzyme β-Gal, we have been able to study the role …
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Cytosolic sphingolipids and lysosome reactivation in neurodegenerative diseases
Glucosylceramide (GlcCer) is one of the simplest glycosphingolipids (GSLs) synthesized at the cytosolic face of cis-Golgi and translocated to the plasma membrane and organelle lumen. The glucocerebrosidases degrade GlcCer in the cytosol and lysosome. GlcCer has been suggested to be an activator of …
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The Role of Ganglioside GD2 and GD3 Synthase (ST8SIA1) in the Regulation of Immune Suppression in Breast Cancer
<p>Gangliosides are acidic glycosphingolipids involved in cell adhesion, proliferation, and modulation of signal transduction pathways. It has been reported that tumor-shed gangliosides influence the activity of immune cells including macrophages, NK cells, and T cells. GD3 synthase (GD3S) is the …
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Dissecting the Roles of Cancer Cell Lipid Metabolism in Tumor Progression and Immune Evasion
… gamma (IFNγ) signaling. Mechanistically, glycosphingolipids impact IFNγ receptor subunit 1 (Ifngr1) localization, mediating IFNγ-induced growth arrest and proinflammatory signaling. Finally, high expression of sphingolipid synthesis genes correlates with poor survival in cancer patients …
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The role of complex gangliosides in glial cell biology
… are a family of sialic acid-containing glycosphingolipids that are enriched in the nervous system. They are located in the outer leaflet of the plasma membrane within lipid rafts and are thought to be involved in numerous cellular events, including proliferation, differentiation, …
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Molecular characterization of the lipidome by mass spectrometry
… sphingolipid species including ceramides, glycosphingolipids and inositol-containing sphingolipids, and sterol lipids including cholesterol. The performance of the methodology was validated by comparing its dynamic quantification range to that of established methodology based on triple …
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The potential of sphingolipid depletion for the treatment of atherosclerosis
… is associated with decreases in plasma glycosphingolipids (GSL). Furthermore, I examined whether SPT inhibition can lead to regression of atherosclerotic lesions. This thesis shows the SPT inhibitor myriocin inhibits atherosclerosis in apoE-/- mice fed a high fat diet. Lesion inhibition …
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Organization and formation of the apical membrane of epithelial cells
… membrane of columnar epithelia is enriched in glycosphingolipids, a class of lipids that are known to interact with cholesterol to form liquid ordered domains, also termed "rafts", in cell membranes. Imaging the apical surface of untreated and raft lipid depleted MDCK cells with …
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Identification and imaging different lipid species within phase-separated model membranes by integration of TOF-SIMS and principal component analysis
… can increase the efficiency of ionization of glycosphingolipids, such as galactosyceramide (GalCer) and glucosylceramide (GlcCer), and PCA of the resulting spectra enables distinguishing and identifying these components according to combinations of mass spectral peaks that are characteristic …
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PLASMA MEMBRANE ASSOCIATED GLYCOHYDROLASES: KINETICS PARAMETER CHARACTERIZATION AND BIOLOGICAL FUNCTION
… qualitative and quantitative changes in glycosphingolipids, together with changes in the expression of the corresponding glycosyltransferases, have been reported along neuronal differentiation and aging (2, 4-8). Using human fibroblasts we set up an HTA (high throughput assay) method able …
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Development and application of methods for analysis of fungal glycosphingolipid structure and functional interactions
… fungal lectins, structural characterization of glycosphingolipids (GSLs) from the fruiting bodies of these mushrooms was performed. The extraction, isolation and purification of the lipids, and their subsequent characterization by nuclear magnetic resonance (NMR), mass spectrometry (MS) and gas …
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Structural analysis of glycolipid-derived oligosaccharides from metabolically radiolabelled colorectal carcinoma SW1116 cells
… the analysis of the carbohydrate portion of glycosphingolipids from colorectal carcinoma cells, SW1116, by metabolically labelling the cells with radioactive monosaccharide precursors. SW1116 cells (1 x 10⁶) metabolically labelled with 222 μCi/ml of either 6-[³H]-D-galactose (25 Ci/mMol) or …
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IS B4GALT5 DEFICIENCY A NEW CONGENITAL DISORDER OF GLYCOSYLATION?
Glycosphingolipids (GSLs) are relevant components of mammalian cell membranes where they play multiple roles. Many specific glycosyltransferase enzymes are responsible for the synthesis of the different oligosaccharide chains of GSLs. Knock-out mouse models lacking single or multiple …
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LIPID COMPOSITION OF MICROGLIAL CELLS AND EFFECTS OF A REMYELINATION-PROMOTING ANTIBODY ON THE SPHINGOLIPID PATTERNS
… obtained in our laboratory showed that different glycosphingolipids were involved in rHIgM22 binding at the cell surface but also that rHIgM22 treatment induced changes in the sphingolipid pattern of oligodendroglial cells (Grassi et al., 2015; S. Grassi et al., 2021). Since microglia seem to be a …